... representation of the
potential referents of a quantifier such as ‘at least
one’ would contain every pair of non-empty sub-
sets of the set of all entities, with a cardinal-
ity on the order of . If ... number of ele-
ments in each of the composed sets (assuming the
sets are sorted in advance and remain so), the cal-
culation of referent sets only adds a factor of
to the si...
... rotation of
the bTD module at the main chain of D606 to dock it
against the calf-2 domain with its C-terminus in prox-
imity of the N-terminus of the b
3
‘open’ TM helix.
Overall evaluation of the ... Authors Journal compilation ª 2010 FEBS
Solution properties of full-length integrin
a
IIb
b
3
refined
models suggest environment-dependent induction of
alternative bent
⁄
extended...
... of cytoplasmic composition of anionic li-
pids in membrane binding of K-Ras was evaluated by
studying the effect of ATP depletion, which inhibits
inward movement of PS, with consequent loss of
plasma ... modifications
of the membrane targeting domain, but also on electri-
cal properties of cell membranes, which in turn
depends on physiological and pathological status of
th...
... localization
of cystatin F, its restricted and readily regulated expres-
sion [18], selective and not too potent inhibition of
cathepsins are all in favor of the active role of cystatin
F in processes of ... dilution,
cystatin F was equilibrated with a twofold molar excess of papain.
The residual activity of papain was measured with Z-FR-AMC. Rel-
ative activity of cystatin F...
... thank Prof. Steve C.
Fry, Institute of Cell and Molecular Biology, Univer-
sity of Edinburgh, Edinburgh, UK, and Dr Albert
Schulte, Ruhr University of Bochum, Germany, for
critical reading of the ... quantities of protein util-
izing the principle of protein-dye binding. Anal Biochem
72, 248–254.
33 Laemmli UK (1970) Cleavage of structural proteins dur-
ing the assembly of the...
... one of a few major Myb-interacting proteins. The
moderate basal level of sumoylation was greatly enhanced
by cotransfection of PIASy, an E3 ligase for SUMO-1.
The functional consequence of abolishing ... to the importance
of the c-Myb–Ubc9 interaction.
We conclude that SUMO-1 conjugation of c-Myb is not
only a phenomenon induced under favourable conditions of
overexpression of...
... physiological activators
are often unknown, and thus the use of an activator
such as SDS is warranted to learn more about the
functional properties of an enzyme. Generally, binding
of SDS during activation ... activity of tarantula hemocyanin E. Jaenicke and H. Decker
1528 FEBS Journal 275 (2008) 1518–1528 ª 2008 The Authors Journal compilation ª 2008 FEBS
Kinetic properties of...
... level of acetylation of
a peptide was estimated on the basis of the intensity ratio of
the native and acetylated species. From the data summar-
ized in Table 1, it can be seen that some of the ... of the monomeric
form of SV-IV is approximately doubled by the addition of
5.4 m
M
SDS (Fig. 1), whereas in the 1–70 region, the a-helix
content increases from 2–3% to 23% in the...
... role of Hsp20. However, investigation of the
biochemical properties of isolated Hsp20 lag behind.
Indeed, the biochemical properties of rat Hsp20 were only
briefly characterized in the reports of ... summa-
tion of the elution profiles of the isolated 3D mutant of Hsp27 and
isolated wild-type Hsp20 is presented on curve 1. Experimental elution
profiles of the mixture of t...