Báo cáo khoa học: Regulation of the muscle-speciﬁc AMP-activated protein kinase a2b2c3 complexes by AMP and implications of the mutations in the c3-subunit for the AMP dependence of the enzyme docx

... complexes by AMP and implications of the mutations in the c3-subunit for the AMP dependence of the enzyme Kerstin Lindgren*, Mattias Ormestad*, Ma˚rten Persson, Soﬁa Martinsson, L. Thomas Svensson and ... important for metabolic sensing in all eukaryotes. The muscle-speciﬁc isoform of the regulatory c -subunit of the kinase, AMP- activated protein kinase c3, has a key role in glucose and fat metabolism ... kinase; AMPKK, AMP- activated protein kinase kinase; CAMKKb,Ca2+⁄ calmodulin-dependent protein kinase b; CBS, cystathionine-b-synthase; TAK1, transforming growth factor b-activating kinase 1;...

Tài liệu Báo cáo khoa học: Regulation of DNA fragmentation: the role of caspases and phosphorylation doc

... is under the direct control of kinases and phosphatases, and the indirect control of phosphorylation through the regula-tion of other apoptotic proteins. Furthermore, manykinases and phosphatases ... phosphorylated protein, the degree of activation and the in uence of other proteins. Phosphorylation signaling pathways are intri-cately interrelated; it was previously shown that either induction or inhibi-tion ... Because of the effect on upstream and downstream proteins as well as the target proteins, wetherefore have to consider the combinations of apopto-sis inducer ⁄ inhibitor, detection method and targetproteins.ConclusionMany...

Tài liệu Báo cáo khoa học: Regulation of the members of the mammalian heat shock factor family doc

... factors, the members of the HSF family are modular proteins composed of functional domains (see ﬁgure 2 in [4]). The most con-served domain is the amino-terminal helix-turn-helixDNA-binding domain ... disturbing the protein homeostasis. As a defence mechanism, HSFs induce the synthesis of HSPs that act as molecular chaperonesthrough binding to the hydrophobic surfaces of unfolded proteins, thereby ... thereby facilitating refolding of pep-tides and preventing protein aggregation [32]. The dis-covery of an interaction between HSF1 and HSPs,such as Hsp70 ⁄ Hsp40 and HSP90, led to the hypothe-sis...

Tài liệu Báo cáo khoa học: Regulation of dCTP deaminase from Escherichia coli by nonallosteric dTTP binding to an inactive form of the enzyme ppt

... between the dCTP(dUTP)-bindingconformer and the dTTP-binding conformer of dCTPdeaminase. We were not able to identify structuralchanges in the main chain of the subunit, or in the interaction of ... both inactive and unfortunatelyno suitable crystals for the structural analysis of V122G could be obtained. However, the conformation of the main chain in the region of residues 120–125 in the ... and B chains in the structure.dTTP binds at the site of the protein shown previouslyto bind the nucleotides dUTP and dCTP in wild-type and the E138A variant [18]. The nucleotide-bindingsite is...

Tài liệu Báo cáo khoa học: Regulation of the actin–myosin interaction by titin doc

... properties, and i t interacts withseveral protein partners such as myosin, a ctin, M protein, C protein, MURF-1, calpain 3, myomesin, a-actinin,nebulin, telethonin and obscu rin. The elastic domains ... experimentally using the Bradfordmethod [44] to measure the protein concentration of the T800-containing solution, using HMM for the standardcurve.MSProteins were in- gel digested by trypsin according ... naturalcomponent of the thin ﬁlament framework, nebulin.Nebulin spans the entire length of thin ﬁlaments and iscapable of modulating the r ate of formation of the a ctin–myosin complex [74,75]. Interestingly,...

Báo cáo khoa học: Regulation of arginase II by interferon regulatory factor 3 and the involvement of polyamines in the antiviral response potx

... spermidine and spermine) throughconversion of l-arginine into l-ornithine [16]. The lat-ter is in turn used by ODC to produce putrescine, the precursor of spermidine and spermine. To further ... are notinduced in response to virus infectionAs the two isoforms of arginase, I (hepatic isoform) and II (extrahepatic isoform), may contribute to the arginase activity measured in the previous ... [16]. The two isoforms possess the same enzymaticactivity for converting l-arginine into l-ornithine and urea, a critical step in the polyamine biosynthesis path-way. Subcellular localization of...

Báo cáo khoa học: Regulation of luteinizing hormone receptor mRNA expression by mevalonate kinase – role of the catalytic center in mRNA recognition potx

... with the site of ATP binding and may exploitcommon side chain interactions; or (b) the conforma-tions of residues involved in RNA binding may bedirectly or indirectly coupled to the binding of ... shown in the crystal structure of ratMVK bound to ATP [17], depicted in Fig. 9A, the active site of the enzyme is located at the interface of the N- and C-terminal domains of the monomer. The adenosine ... number of known RNA binding proteins, the exact details of the mode of interaction betweenRNA and this motif still remain somewhat unclear.Inspection of two other left-handed b–a–b motif-con-taining...

Báo cáo khoa học: Regulation of translational efﬁciency by different splice variants of the Disc large 1 oncosuppressor 5¢-UTR potx

... condi-tions, by the Jun N-terminal kinase, the P38c MAP kinase, the cyclin-dependent kinases 1 and 2 and the PDZ-binding kinase, resulting in changes in distribu-tion and stability of the protein [20–23]. ... translational efﬁciency by acting as binding sites for some regulatory proteins or by inhibiting the bind-ing or scanning of the translational machinery [27].Then, we were interested in whether RNA secondarystructure ... altered in cancers of epithelial origin and is the target of oncogenic HPV,we were ﬁrst interested in investigating the initiation of DLG1 transcription in epithelial cells [15,16]. The results of...

Báo cáo khoa học: Regulation of output from the plant circadian clock pot

... upon germination(Fig. 1B). The rate of hypocotyl growth is greatest in the evening and minimal in the morning, and can beentrained by light even before the cotyledons emergefrom the seed coat ... expression of mRNA in these pathways isalways matched by circadian regulation at the level of protein synthesis. However, in some cases protein lev-els are under circadian control, for example, the ... isunstable in the dark. By contrast, during the day far-red and blue light stabilize CO protein, and COaccumulates in the nucleus [41]. CO then activates the transcription of the ﬂoral regulator...

Báo cáo khoa học: Regulation of the human leukocyte-derived arginine aminopeptidase/endoplasmic reticulum-aminopeptidase 2 gene by interferon-c pot

... Eklund EA & Kakar R (1999) Recruitment of CREB-binding protein by PU.1, IFN-regulatory factor-1, and the IFN consensus sequence-binding protein is necessary for IFN-c-induced p67phox and gp91phox ... IRF-1 is a candidate for the mediator of IFN-c action. In contrast, no effect wasobserved on the expression of IRF-2 protein. To determine whether IRF-1 and -2 bind to the IRF-E site in the L-RAP ... IFN-c-inducible IRF-E binding protein other thanIRF-1 and IRF-2 was detectable by EMSA, even in the basal condition. Identiﬁcation and elucidation of the role of this protein in L-RAP gene expression...

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