Báo cáo khoa học: Nop53p interacts with 5 8S rRNA co-transcriptionally, and regulates processing of pre-rRNA by the exosome ppt

Báo cáo khoa học: Nop53p interacts with 5.8S rRNA co-transcriptionally, and regulates processing of pre-rRNA by the exosome ppt

... processing and toco-immunoprecipitate the 27S and 7S pre-rRNAs and the mature 5. 8S rRNA [31–33], and to bind 5. 8S rRNA in vitro [31]. In order to determine whether Nop53p interacts with the pre -rRNA ... degraded by the exosome in the absence of Nop53p. In order to determine whether the effect of Nop53p depletion on 5. 8S processing by the exosome was indi-rect, or whether it involved direct exosome ... 5B). The strain expressingC -Nop53p showed higher levels of 7S pre -rRNA and lower levels of the mature 5. 8S rRNA (Fig. 5B). Quanti-ﬁcation of 7S ⁄ 5. 8S ratio in these strains showed thatN-Nop53p...

Báo cáo khoa học: CD91 interacts with mannan-binding lectin (MBL) through the MBL-associated serine protease-binding site doc

... inves-tigate whether interaction with CD91 takes place ator near the MASP-binding site, the ability of the MBL mutants K55A and K55Q, which lack the abil-ity to associate with MASPs, to interact with ... concentration of active CD91 and there-fore the kinetics of the interaction when CD91 is insolution. The KDvalue obtained was 9.33 nm with a v2= 0.899, and kd and kavalues of 5. 74 · 10)4S)1 and ... sur-face and biotinylated CD91 was added for the indicated intervals of time in 25 mM Tris ⁄ HCl, 0 .5% Tween 20, 150 mM NaCl (pH 7 .5) or 25 mM Tris ⁄ HCl, 150 mM NaCl, 5 mM CaCl2(pH 7 .5) . The...

Báo cáo khoa học: NBR1 interacts with fasciculation and elongation protein zeta-1 (FEZ1) and calcium and integrin binding protein (CIB) and shows developmentally restricted expression in the neural tube pptx

... conserved, with 87% sequencesimilarity with the m urine homologue. The murine Nbr1 geneliesheadtoheadwiththeBrca1 gene, with the intragenicregion of 289 bp suggesting the possibility of co-ordinatedexpression ... at 55 °C. The s lides were then washedat high stringency (20 min at 55 °Cin2´ NaCl/Cit, 50 %formamide,10 mMdithiothreitol) and then treated with 40 lgámL)1RNAse A for 30 min at 37 °C. The ... of Medicine, Guy's Hospital, London SE19RT. Fax: + 020 7 955 8762, Tel.: + 020 7 955 5000 ext. 55 85. E-mail: caroline.whitehouse@kcl.ac.ukAbbreviations: NBR1, next to BRCA1; CIB, calcium and...

Báo cáo khoa học: Nucling interacts with nuclear factor-jB, regulating its cellular distribution potx

... mechanism underlying the regulation of this pathway by Nucling remains to beelucidated. The classic form of NF- jB is the heterodimer of the p50 and p 65 subunits, which is sequestered in the cyto-plasm ... overexpressed by cotrans-fection (Fig. 5B,C,F,G). However, when NuclingDNwas coexpressed with NF-jB (p50 or p 65) , over 65% of cells showed nuclear staining of NF-jB (p50 orp 65) (Fig. 5D,H). The cytosolic ... regulate the nuclear translocation of endogenousNF-jB, we investigated the endogenous distribution of NF-jB (p 65 and p50) in WT and Nucling)/)MEFs(Fig. 5K–N,K¢–N¢). In WT MEFs, both p50 and p 65 were...

Báo cáo khoa học: UXT interacts with the transcriptional repressor protein EVI1 and suppresses cell transformation ppt

... 2007)doi:10.1111/j.1742-4 658 .2007. 059 28.x The EVI1 transcriptional repressor is critical to the normal development of a variety of tissues and participates in the progression of acute myeloid leu-kaemias. The repressor ... that the gene nor-mally encodes a putative protein of 157 amino acids with a predicted molecular mass of 18.2 kDa. Interro-gation of the NCBI nucleotide database revealed the identity of A1 with ... 3963bacterially expressed and 35 S-methionine-labelled pro-teins and the results are shown in Fig. 3. These con-ﬁrm that UXT binds Rp (Fig. 3B, lane 5) and furthermore also interacts with full-length...

Báo cáo khoa học: Dematin interacts with the Ras-guanine nucleotide exchange factor Ras-GRF2 and modulates mitogen-activated protein kinase pathways doc

... structure of the Dbl and pleckstrinhomology domains from the human Son of sevenless protein. Cell 95, 259 ±268.36. Hall, A. (1998) Rho G TPases and the actin c ytoskeleton. Science279, 50 9 51 4.37. ... triggered by t heGbc 25. Moreover, the co-expression of Ras-GRF1 and Gbcsubunits leads to the activation of the MAP kinases JNK1 and ERK2 in heterologous cells [ 25] . R as-GRF2 stimulates the ERK1 ... between Ras signaling and the actin cytoskeleton.Elucidation of the crystal structure of tandem DH a ndPH domains of human Sos1 protein highlights the dramaticcomplexity of the DH domain±mediated...

Báo cáo khoa học: IMP1 interacts with poly(A)-binding protein (PABP) and the autoregulatory translational control element of PABP-mRNA through the KH III-IV domain pdf

... that the pres-ence of the two terminal short stretches of adenines at the 5 and the 3¢ ends of the ARS were not essentialfor binding to IMP1 (Fig. 3B: lanes 2 and 3, and the sequence of ARS and ... structure of 5. 8S rRNA from a thermo-phile, Thermomyces lanuginosus. J Biol Chem 256 , 56 75 56 82.38 Caputi M & Zahler AM (2001) Determination of the RNA binding speciﬁcity of the heterogeneous ... Fig. 3A). The 20nucleotide long region of the 5 end of the ARS(DARS-L; Fig. 3B: lane 4) and the A and U richregion located at the middle segment of the ARS(DARS-C; Fig. 3B: lane 5) were unable...

Tài liệu Báo cáo khoa học: Interleukin-1-inducible MCPIP protein has structural and functional properties of RNase and participates in degradation of IL-1b mRNA doc

... acti-vated by many stimulants, and regulates the level of IL-1b mRNA and of its own transcript. Further stud-ies are necessary to clarify the biological role of MCPIP as a nuclease and to identify the ... Structures of the PIN domains of SMG6 and SMG5 reveal a nuclease within the mRNA surveillancecomplex. EMBO J 25, 51 17 51 25. 8 Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F& Higgins DG (1997) The ... to shortening of the IL-1b transcript half-life, and are not related to the presence of AU-rich elements in the 3¢ UTR. The interaction of the MCPIP with transcripts of both IL-1b and MCPIPobserved...

Tài liệu Báo cáo khoa học: "Japanese Dependency Parsing Using Co-occurrence Information and a Combination of Case Elements" pdf

... the set of case elements that modify the i-th verb in Trsi(T ) : the set of particles in the set of case el-ements that modify the i-th verb in Tnsi(T ) : the set of nouns in the set of ... )) the co-occurrence prob-ability of the particle set and the verb, and wecall P(ni,j(T )|ri,j(T ), vi(T )) the co-occurrenceprobability of the case element set and the verb.In the ... dimension of the hidden classz was set to 300. As a result, the collected n, r, vtotal 102 ,58 1,924 pairs. The number of n and v is 57 ,3 15 and 15, 098, respectively. The particles for which the co-occurrence...

Báo cáo khoa học: Dạy trích đoạn tiểu thuyết tự truyện của M.Gorki theo thi pháp thể loại ppt

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