Báo cáo khoa học: Downregulation of protease-activated recep

Báo cáo khoa học: Downregulation of protease-activated receptor-1 in human lung ﬁbroblasts is speciﬁcally mediated by the prostaglandin E2 receptor EP2 through cAMP elevation and protein kinase A pot

... mechanisms for CCN2 ⁄ CTGF persistence in fibrotic gingiva: interactions between cAMP and MAPK signaling pathways, and prostaglandin E2- EP3 receptor mediated activation of the c-JUN N-terminal kinase. ... 2008 The Authors Journal compilation ª 2008 FEBS 3679 Downregulation of protease-activated receptor- 1 in human lung fibroblasts is specifically...

Ngày tải lên : 30/03/2014, 04:20

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Báo cáo khoa học: Internalization of cystatin C in human cell lines docx

... B and L involved in cancer growth and metastasis [2]. Since the involvement of cathepsin B in cancer metastasis was originally described by Sloane et al. [3], cathepsins, and especially cathepsin ... (repre- senting intracellular cystatin C) was quantiﬁed by ELISA and the cystatin C level was correlated to the protein content of the cell lysate. The lines are...

Ngày tải lên : 16/03/2014, 06:20

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Báo cáo khoa học: A chloroplast RNA binding protein from stromal thylakoid membranes speciﬁcally binds to the 5¢ untranslated region of the psbA mRNA potx

... mRNA corresponding to positions )147 to +24 relative to the AUG): T7-psbB5¢ (5¢-GTAATACGACTCACTATAGGGTAAATTAATT TAATTTAAAATC-3¢)andpsbB3¢ (5¢-TACACGATA CCAAGGTAAACC-3¢). Each template contained ... psbA mRNA corresponding to positions )91 to +13 relative to the AUG); T7-psbA5¢ (5¢-GTAATACGACTCA CTATAGGGTACCATGCTTTTAATAGAAG-3¢ )and 2054 (5¢-GATCCATGGTCATATGTTAATTTTTTTAA AG-3¢); )36-RNA (w...

Ngày tải lên : 17/03/2014, 11:20

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Báo cáo khoa học: Characterization of sequence variations in human histone H1.2 and H1.4 subtypes pptx

... cell lines and tissues investigated contained only alanine at this location. To confirm the H1.2 Ala17Val sequence variation, a 183 bp PCR product was amplified in the 5¢-UTR, and the start of the ... had a specific role in DNA damage-induced apoptosis [14]. It is useful therefore to examine the properties and expression of these variants as this furthers a better...

Ngày tải lên : 30/03/2014, 20:20

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Báo cáo khoa học: K4, K9 and K18 in human histone H3 are targets for biotinylation by biotinidase pdf

... panel). The nuclear compartment was stained using DAPI, and the cytoplasm was stained using rhodamine phalloidin. Images entitled ‘Merged’ were created by overlaying images obtained by staining with ... DNA and positively charged e-amino groups (lysine residues) and guanidino groups (arginine residues) in histones. Histones consist of a globular C-terminal domain and a...

Ngày tải lên : 23/03/2014, 15:20

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Tài liệu Báo cáo khoa học: Role of Kupffer cells in pathogenesis of sepsis-induced drug metabolizing dysfunction pptx

... against hepatic drug-metabolizing dysfunction by modulation of the in ammatory response. Abbreviations ALT, alanine aminotrasferase; AST, aspartate aminotrasferase; CLP, cecal ligation and puncture; ... KCs [25]. The expression and activity of CYP2E1 were downregulated in a rat hepatoma cell line after the administration of proinﬂammatory cytokines, leading to a loss...

Ngày tải lên : 14/02/2014, 18:20

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Tài liệu Báo cáo khoa học: Interruption of triacylglycerol synthesis in the endoplasmic reticulum is the initiating event for saturated fatty acid-induced lipotoxicity in liver cells pdf

... Horse- radish peroxidase-conjugated antibody against caspase-3 (#610325) was from BD Pharmigen, and antibody against b-actin (A5 441) was from Sigma. Statistical analysis All data are expressed as the ... unsaturated FFAs into the cells. After their internalization, FFAs are converted to fatty acyl-CoA, a reaction catalyzed by ACS. Fatty acyl-CoAs are activated forms of fatty ac...

Ngày tải lên : 14/02/2014, 22:20

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Tài liệu Báo cáo khoa học: Role of ceramide kinase in peroxisome proliferatoractivated receptor beta-induced cell survival of mouse keratinocytes ppt

... 5¢-GTAGGCATGAGAACGGGA AG-3 and for reverse 5¢-GGGGGTAAGAGGAGGAGA AA-3¢ and for CERK-negative forward 5¢-CCGCAAG AGGCTTTATTGTC-3 and reverse 5¢-TATGCCAAGGA CACGGAGAT-3¢, as a negative control PCR primer. The condition ... which encodes a region of the protein that is known to be essential for kinase activity and contains a diacylglycerol kinase- like catalytic domain...

Ngày tải lên : 18/02/2014, 18:20

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Báo cáo khoa học: Roles of conserved arginines in ATP-binding domains of AAA+ chaperone ClpB from Thermus thermophilus pptx

... (AAA-1), a middle domain, and a second AAA+ module (AAA-2). Each AAA+ module contains highly conserved WalkerA and WalkerB motifs, and two arginines (AAA-1) or one arginine (AAA-2). Here, we investigated ... arginine of AAA- 1, R33 2A, decreased the chaperone activity and desta- bilized the hexamer, and that the alanine mutation of the P1 arginine of AAA-2, R75 6A,...

Ngày tải lên : 06/03/2014, 00:20

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Báo cáo khoa học: Involvement of lysine 1047 in type I collagen-mediated activation of polymorphonuclear neutrophils doc

... activation mechanism and conﬁrm the prominent involvement of lysine residues in interactions between extracellular matrix proteins and in ammatory cells. Abbreviations AHA, 6-amino-hexanoic acid; ... Boonmark N, Skocir P, Lawn R & Plow EF (1996) A quantitative immunoassay for the lysine-binding function of lipoprotein (a) . Application to recombinant apo (a) and lipopro...

Ngày tải lên : 07/03/2014, 06:20

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