Báo cáo khoa học: Analysis of the CK2-dependent phosphorylation of serine 13 in Cdc37 using a phospho-speciﬁc antibody and phospho-afﬁnity gel electrophoresis doc

... Koyasu S, Nishida E, Kadowaki T, Matsuzaki F, IidaK, Harada F, Kasuga M, Sakai H & Yahara I (1986)Two mammalian heat shock proteins, HSP90 and HSP100, are actin-binding proteins. Proc Natl ... [1]. Therefore, the analysis of protein kinaseactivity and the phosphorylation level of their sub-strates are important for understanding signal trans-duction pathways at a molecular level. Many ... regulated in manydifferent ways. Releasing an inhibitory subunit from a catalytic subunit can activate a kinase. By contrast,binding an activating regulatory coprotein to an inac-tive catalytic...

Tài liệu Báo cáo khoa học: nsights into the reaction mechanism of glycosyl hydrolase family 49 Site-directed mutagenesis and substrate preference of isopullulanase doc

... GGTGCTgAGCTCAAGTGTGACTTtcGTCTACSacIW402F CCGGTGGTcGAcTTTGGTTtcACGCCCSalIE273Q ACGTACTGCTgTCCGGAAAGtACtCCATGGCCGCScaID353N TCCAATCCGTtAGTCTGgCCaTAGAACGCGCMscIE356Q GGAGAATGGTgCCAGGGTACATTTcCAATCCGTCAKpnID372N ... of 3.2) [45] was a solution in the Dali result. It islikely that the inte rface between N-terminal and b-he licaldomains participate in binding of the polysaccharide,pullulan.Comparison of ... compar e the mechanism of h ydrolysis of IPU and o therGH family 49 dextranases, the a nomer conﬁguration of the hydrolysate of IPU was determined. A polarimetric assaywas carried out using panose...

Tài liệu Báo cáo khoa học: "Methods for the Qualitative Evaluation of Lexical Association Measures" doc

... Evaluation of Association Measures2.1 State -of- the- art A standard procedure for the evaluation of AMs ismanual judgment of the -best candidates identi-ﬁed in a particular corpus by the measure ... curves. The major drawback of an approach where alllow-frequency candidates are excluded is that a large part of the data is lost for collocation extrac-tion. In our data, for instance, 80% of the ... low-frequencycandidates).4 Experimental SetupAfter extraction of the base data and manual iden-tiﬁcation of TPs, the AMs are applied, resulting in an ordered candidate list for each measure (hence-forth...

Báo cáo khóa học: Insight into the activation mechanism of Bordetella pertussis adenylate cyclase by calmodulin using ﬂuorescence spectroscopy pptx

... of the 3D structures of AC and AC–CaM,which are still not available, and labeling of the AC protein atother locations in its regulatory and catalytic domains using single-cysteine mutants are ... site in both the uncomplexed AC and the AC–CaM complex.Dynamics of the AC catalytic domain as probedby acrylodanTaking advantage of the absence of Cys residues in the native AC protein, the insertion ... the rapid re-activation of AC after denaturation by urea, and on the fact that AC and CaM differ largely in their isoelectric points, allowingthem to be separated either in free form or in a...

Báo cáo khoa học: Bacitracin inhibits the reductive activity of protein disulﬁde isomerase by disulﬁde bond formation with free cysteines in the substrate-binding domain pptx

... Cys345 and the thiol form of bacitracin A. The thiol form of the thiazoline ring of bacitracin A and Cys345are shown as chemical structures, the rest of bacitracin A as ‘Bacitracin A , and all other ... domains, a, b, b¢ and a , and anx-linker region between the b¢-domain and a -domain. The a- domains are catalytically active, the b¢-domainfunctions as a substrate-binding domain displaying a large ... Separation of bacitracin analogs. (A) Structures of the most abundant bacitracin analogs of commercial bacitracin mixtures including the amino-thiazoline ring (a) and (b) or keto-thiazole ring...

Báo cáo khoa học: Intrabodies against the EVH1 domain of Wiskott–Aldrich syndrome protein inhibit T cell receptor signaling in transgenic mice T cells potx

... coding linkercontaining a NotI site at the 5¢ end of the linker (5¢-GGCCGCAGGTTCGGAGCAGAAGCTGATCAGCGAGGAGGACCTGTAG-3¢) and noncoding linker containing anEcoRI site at the 5¢ end of the linker ... 5¢-CACCCAAGCTTGCCACCATGCAGGTTACTCTGAAAGAGTC-3¢; primer 4, 5¢-CACCCAAGCTTGCCACCATGAAATGCAGCTGGGTTATCTTC-3¢; primer 5, 5¢-CAGAACCACCACCCCCTGAGGAGACGGTGACTGAGGTTCC-3¢; primer 6, 5¢-CACCCAAGCTTGCCACCATGGAGGTTCAGCTGCAGCAGTCTG-3¢; ... 9,5¢-GGTGGAGGAGGTTCTGATGTTGTTCTGACCCAAACTCCACTC-3¢;primer 10, 5¢-CGAATGCGGCCGCCCGTTTCAGCTCCAGCTTGGTCC-3¢;primer 11, 5¢-TCAAAACATCAGAACCTCCTCCACCGGATCCTCCACCTCCAGAACCACCACCCCC-3¢; primer 12, 5¢-GAACAACATCAGAACCTCCTCACCGGATCCTCCACCTCCAGAACCACCACCCCC-3¢;...

Báo cáo khoa học: Principles behind the multifarious control of signal transduction ERK phosphorylation and kinase/phosphatase control potx

... FluorSTMMultiImager (Bio-Rad) and quantiﬁed using the multi-analist software (Bio-Rad). All measurements were carriedout in the linear range of the method. The standard error of the mean was 4.6% of the ... cellular signaling pathways such as the MAPK cascade. The ﬁrst kinase was activated by a receptor that switches slowly between an active and an inactive state.As the duration and amplitude of signaling ... light on the control of kinases and phosphatases on signal transduction [22].For instance, it was predicted that, in a protein kinasesignaling pathway, kinases mainly control signalingamplitude,...

Báo cáo khoa học: Studies on the regulatory properties of the pterin cofactor and dopamine bound at the active site of human phenylalanine hydroxylase pptx

... the hPAHÆadrenaline/dopamine binary complex [17] was superimposed onto that of the ligand-free rPAH containing the regulatory and catalytic domains [19] the catecholamine main-chain is alsoFig. ... by severalmechanisms in order to maintain the phenylalanine and tyrosine homeostasis in vivo despite great ﬂuctuations in the dietary intake of L-Phe and the overall rate of proteincatabolism. ... structures of the binary and ternarycomplexes of hPAH have provided a detailed picture of the protein contacts involved in the binding of biopterincofactor and amino acid substrate at distinctly...

Báo cáo khoa học: Curcumin suppresses the dynamic instability of microtubules, activates the mitotic checkpoint and induces apoptosis in MCF-7 cells ppt

... curcuminincreased the accumulation of Mad2 and BubR1 at the kinetochores, indi-cating that it activated the mitotic checkpoint. In addition, curcumin treat-ment increased the metaphase ⁄ anaphase ratio, indicating ... increase in the metaphase ⁄ anaphase ratio. The metaphase ⁄ anaphaseratio was calculated to be 0.43 ± 0.06 and 1.88 ± 0.40 (P < 0.0001) in the absence and presence of 24 lm curcumin, supporting ... with curc-umin and vinblastine or paclitaxel for one cell cycle. The combination index was calculated using the Chou and Tala-lay method [26,52], with the help of the following equationCI ¼ðDÞ1=ðDxÞ1...

Báo cáo khoa học: Evidence for the slow reaction of hypoxia-inducible factor prolyl hydroxylase 2 with oxygen pptx

... MJ, Padmakumar R & Hausinger RP (1999)Stopped-ﬂow kinetic analysis of Escherichia coli tau-rine ⁄ alpha-ketoglutarate dioxygenase: interactions withalpha-ketoglutarate, taurine, and oxygen. ... Hausinger RP (2005) Steady-state and transientkinetic analyses of taurine ⁄ alpha-ketoglutarate dioxy-genase: effects of oxygen concentration, alternative sul-fonates, and active-site variants ... stable Fe(II)-containingintermediates, as well as the characterization of reac-tion intermediates, including the Fe(IV)=O complex and the Fe(II) product complex, by a combination of rapid kinetic...

Xem thêm