Báo cáo khoa học: Effect of ecdysone receptor gene switch ligands on endogenous gene expression in 293 cells docx

... signiﬁ-cant changes in the expression of host genes.AMK, RG-121150, affects gene expression in 293 cells The affect of the AMK RG-121150 on gene expression in 293 cells was analyzed using microarray ... changes in expression of endogenous genes in 293 cells andtherefore should have minimum pleiotropic effects when used as ligands for the ecdysone receptor gene switch. AbbreviationsAMK, amidoketone; ... inducers of ecdysone receptor switches on the expression of endogenous genes in HEK 293 cells was determined. Four ligand chemotypes, represented by a tetrahydroquin-oline (RG-120499), one amidoketone...

Báo cáo Y học: Effects of ATP depletion and phosphate analogues on P-glycoprotein conformation in live cells docx

... accompanied bycomplementary changes in MM12.10 binding also in re-sponse to direct modulation of the ATP-binding site, con-ﬁrming that conformational changes intrinsic to the catalyticcycle are ... UIC2binding in the presence of substrates/modulators is appliedas an indicator of the expression of functional Pgpmolecules in clinical tissue samples [20]. Changes in theproteolysis proﬁle of ... data)1interactwithPgpinsuchamannerthatpreincubation of the cells with UIC2 completely suppressesthe subsequent binding of MM12.10. In contrast, UIC2mAb added at saturating concentration decreased...

Báo cáo khoa học: Improvement of ecdysone receptor gene switch for applications in plants: Locusta migratoria retinoid X receptor (LmRXR) mutagenesis and optimization of translation start site pptx

... conditional regulation of transgene expression bythe interaction of a chemical inducer with a designed receptor or transcription factor is a powerful tool forthe regulation of transgenes in ... undetectable level of transgene expression in the absence of a chemicalligand, followed by rapid and robust induction of transgene expression in the presence of a low concen-tration (nanomolar) of a chemical ... DNA-binding domain with CfEcR ligand-binding domain cloned under the control of the 35S promoter in the pKYLX80 vectorLmRXR A fusion of VP16 activation domain and LmRXR ligand-binding domain cloned under...

Báo cáo khoa học: "Effect of intern’s consecutive work hours on safety, medical education and professionalism"

... rapidly increases after 16 hours of sustainedwakefulness, a finding reflected in the twofold increase in interns’ attentional failures after they had been working formore than 16 hours on the ... comments, including clarification of thepower, sample size, and statistical approach descriptions thatappeared in our recent Journal Club review [1] of theirstudies examining the effect of reducing ... of disciplines. We likewise agree that optimizing patient hand-offs, medical education, and trainees’ sense of professionalism should be priorities as interventions aredeveloped that reduce consecutive...

Tài liệu Báo cáo khoa học: Effect of sequence polymorphism and drug resistance on two HIV-1 Gag processing sites pdf

... 2).Increasing the concentration of the NC/p1 substrate abovethe Kmresulted in a decreased velocity, suggesting thepossibility of increased nonproductive binding at thesubstrate binding ... residues are in bold.bThe type of mutation: natural polymorphism (P), mutation appearing only in drug resistance (R, seeFig. 1). If two mutations occurred and one of them was occurring only in drug ... 6300amino-acid analyzer. Stock solutions and dilutions weremade in distilled water and the peptide concentrations weredetermined by amino-acid analysis.Construction and puriﬁcation of HIV-1...

Báo cáo khoa học: Effect of mutations at Glu160 and Val198 on the thermostability of lactate oxidase doc

... Thesecondary-structure nomenclature is according to Lindqvist [19]. The picture was created usingINSIGHT IIsoftware (Version 4.3, Accelrys Inc.) on anONYX2 workstation (Silicon Graphics, Inc.).Fig. ... forming a hydrogen-bond network betweenthe NH2 of Arg143 and the main-chain carbonyls of Lys181and Asn182 directly, and the main-chain carbonyl of Glu184 by way of a water molecule. However, in ... bicinchoninic Protein Assay RegentKit (Pierce) using puriﬁed bovine serum albumin as thestandard. Irreversible enzyme inactivation was measured ata protein concentration of 50 lgÆmL)1 in a 40 mMHepesbuffer,...

Báo cáo khoa học: Interaction between catalytically inactive calpain and calpastatin Evidence for its occurrence in stimulated cells docx

... presence of Ca2+, the addition of equimolar amounts of calpasta-tin induces an increase of approximately one order of magnitude in the afﬁnity of calpain for mAb 56.3,indicating that, in these conditions, ... for interaction with calpain.This hypothesis is conﬁrmed by the effect of arachi-donate treatment on the intracellular distribution of calpastatin (Fig. 8). In untreated cells, cytosol containsa ... higher concentra-tions of the metal ion [45]. The addition of E64, a syn-thetic inhibitor of calpain, did not modify the intensity of the signal observed in the presence of Ca2+alone.The increase...

Báo cáo khoa học: Cdk2 activity is dispensable for triggering replicon initiation after transient hypoxia in T24 cells docx

... elevated concentration of staurosporine.Effects of Cdk2 inhibition on pRb, Cdc6 andPCNA in vivo In Fig. 4 we analysed the effects of the Cdk2 inhibitorsolomoucine, roscovitine and staurosporine ... incubation condition (data notshown). This suggests that in T24 cells inhibition of the pRb pathway is not the direct cause of the hypoxicsuppression of initiation in replicons. Moreover, in cells ... emergence of additional replicating nuclei are in the order of hours. In contrast, our protocol produces, in living cells, a deﬁned state of replicons situatedbefore origin unwinding (as shown in the...

Tài liệu Báo cáo khoa học: Effect of gadolinium on the ryanodine receptor/ sarcoplasmic reticulum calcium release channel of skeletal muscle docx

... thevisualization of the protein composition of the fractions.Ryanodine-bindingRyanodine-binding assays were carried out using [3H]ry-anodine in a medium having the composition of 1 m NaCl,S. ... released. The effect of 5 lm gadolinium on the[3H]ryanodine binding to the HSR was investigated atdifferent ryanodine concentrations. Gadolinium (5 lm)Table 1. The effect of cis gadolinium on the ... equation resulting in a half-inhibitory concentra-Fig. 1. Effect of cis gadolinium on the ryanodine receptor. Singlechannel records were taken at )108 mV using symmetrical bathingsolution having...

Báo cáo khoa học: " Effect of the medical emergency team on long-term mortality following major surgery"

... glycopeptides only, and Stenotropho-monas maltophilia, sensitive to ofloxacin only. Polymicrobialwound contamination also included contamination with fungi(for instance, Candida albicans as well as non-albicans ... tractTsunami near-drowning involved the aspiration of immersionfluids as well as marine and soil debris into the respiratorytract, thus producing intrapulmonary inoculation of bacteria. In accordance, ... pre-existing con-ditions [2].ConclusionSevere large scale soft-tissue damage, including high-levelcontamination, was common to all tsunami victims repatriatedfrom the disaster area. During the interim...

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