... reverse,5¢-GGGGGATCCCAAGTACTGTT-3¢) and GAPDH (for-ward, 5¢-CCCATCACCATCTTCCAGGA-3¢; reverse, 5¢-TGCTTCACCACCTTCTTGAT-3¢)at94 C for 30 s,56 C for 15 s and 72 C for 2 min, for 30 cycles. The expected ... synergistic effect on the induction of cytokines⁄chemokinesTo determine whether there is any combined effect of AGE-HSA and LPS on the induction of cytokines ⁄chemokines, we stimulated HUVECs with ... (forward, 5¢-CAGGAGCCCAGCTATGAACT-3¢; reverse, 5¢-TAAGTTCTGTGCCCAGTGGA-3¢),IL-8 (forward, 5¢-AGGGTTGCC AGATGCAATAC-3¢;reverse, 5¢-ACACAGCTGGCAATGACAAG-3¢), MCP-1(forward, 5¢-GTGAGGAGCCACCAACATTT-3¢;...