Báo cáo khoa học: Synergistic co-operation of signal transducer and activator of transcription 5B with activator protein 1 in angiotensin II-induced angiotensinogen gene activation in vascular smooth muscle cells potx

Tài liệu Báo cáo khoa học: Peptides corresponding to helices 5 and 6 of Bax can independently form large lipid pores pdf

... 15 2 10 2 7 1 19 4 26 320% TFE 62 5 26 2 0 1 0 1 3 1 9 660% TFE 61 5 27 2 0 1 0 2 1 1 11 50.5 mM SDS 63 6 19 3 1 2 0 1 4 4 13 44mM SDS 61 4 25 3 0 1 0 1 4 2 10 6Bax-a6 Buffer 13 1 10 1 ... PolydispersityPeak intensity(kc ⁄ s)None 0 13 0.3 ± 1. 5 0 .11 ± 0.05 14 1.2 ± 3.6Bax-a5 500 12 9.6 ± 1. 2 0 .12 ± 0.03 14 7.0 ± 3.5250 13 1.4 ± 1. 2 0 .12 ± 0.03 14 9.0 ± 4.450 14 0.3 ± 0.9 0 .17 ± 0.04 15 9.6 ± ... 10 1 20 4 9 1 20 2 28 320% TFE 27 4 15 0.2 8 1 6 0.2 18 2 26 260% TFE 40 4 22 2 2 1 3 1 12 3 21 20.5 mM SDS 33 3 16 0.2 8 1 5 0.3 15 2 23 34mM SDS 42 1 19 0.3 3 1 2 0.3 11 1 22 1 Large pores...

Tài liệu Báo cáo khoa học: Arginine ethylester prevents thermal inactivation and aggregation of lysozyme pptx

... anadditive to prevent inactivation and aggregation of heat-labile proteins.Keywords: arginine; arginine ethylester; lysozyme; protein aggregation; thermal inactivation. Protein a ggregation is ... theproblems of protein aggregation and misfolding. Recentlywe reported that polyamines, typically spermine and spermidine, prevent heat-induced inactivation and aggre-gation of lysozyme [18 ,19 ]. As ... 4. 81 ± 0.44 2. 61 ± 0.38Spermine 1. 22 ± 0 .13 0.57 ± 0 .14 Arg 4.24 ± 0.39 2 .17 ± 0.25ArgEE 1. 15 ± 0 .15 0.42 ± 0.080.2 mgÆmL )1 (pH 7 .1) None 4.03 ± 0.24 1. 11 ± 0.06Arg 1. 62 ± 0.25 1. 09 ± 0.06ArgEE...

Tài liệu Báo cáo khoa học: The undecided serpin The ins and outs of plasminogen activator inhibitor type 2 pdf

... expression of tissue- and urinary-type plasminogen activator and plasminogen activator inhibitor 1 and 2. J Cell Biol 10 6, 9 71 978.84 Medcalf RL (19 92) Cell- and gene- speciﬁc interactionsbetween signal ... binding sites for severalARE binding proteins, including the stabilizing protein HuR [86] and the mRNA destabilizing protein tristetr-aprolin (TTP) [87]. HuR is a member of the Hu family of ... 76, 812 – 819 .45 Chuang TL & Schleef RR (19 99) Identiﬁcation of anuclear targeting domain in the insertion betweenhelices C and D in protease inhibitor -10 . J Biol Chem274, 11 194 11 198.46...

Báo cáo khoa học: Evolutionary changes to transthyretin: structure and function of a transthyretin-like ancestral protein doc

... families of proteins were in theN-terminal and C-terminal regions [11 ]. In order todistinguish between the two protein families in greaterdetail, a comparative analysis of TLP and transthy-retin ... Richardson SJ(2006a) Structural and functional evolution of transthy-retin and transthyretin-like proteins. Proteins: StructFunct and Bioinf 64, 10 24 10 45. 12 Ramazzina I, Folli C, Secchi A, Berni ... Evolution of the thyroidhormone-binding protein, transthyretin. Gen CompEndocrinol 11 9, 2 41 255.2 Richardson SJ (2007) Cell and molecular biology of transthyretin and thyroid hormones. Int Rev...

Báo cáo khoa học: Post-ischemic brain damage: pathophysiology and role of inﬂammatory mediators ppt

... ischemic injury and pain. J Pharmacol Sci 10 0, 4 61 470. 11 1 Allan SM & Rothwell NJ (20 01) Cytokines and acuteneurodegeneration. Nat Rev Neurosci 2, 734–744. 11 2 Rothwell N (2003) Interleukin -1 and ... increase very early following permanentMCAO [11 5 ,11 6] and peak within hours of reperfusion in transient focal ischemic models in rodents[97 ,11 7 ,11 8]. The main source of the cytokine after cere-bral ... Immunol 15 8, 18 18 18 24. 13 9 Herzog C, Kaushal GP & Haun RS (2005) Generation of biologically active interleukin -1 beta by meprin B.Cytokine 31, 394–403. 14 0 Pinteaux E, Inoue W, Schmidt L, Molina-Holgado...

Báo cáo khoa học: pH dependence, substrate speciﬁcity and inhibition of human kynurenine aminotransferase I ppt

... BhKAT-IKmmMkcatmin )1 kcat/Kmmin )1 ÆmM )1 kcat/Kmmin )1 ÆmM )1 kcat/Kmmin )1 ÆmM )1 kcat/Kmmin )1 ÆmM )1 ratelmolÆmin )1 Æmg )1 Glutamine 2.8 ± 0.5 440.5 ± 28.7 15 7.3 4 14 7.8 0.04 1. 8Phenylalanine 1. 7 ± 0.3 91. 0 ... 0.9 215 .4 ± 14 .4 33.7 6.3 11 6.6 0. 012 < 0.05Tyrosine 3.2 ± 0.4 91. 0 ± 4.8 28.4 200 15 4.7 0.00 31 < 0.05Histidine 5.4 ± 1 143.6 ± 14 .4 26.6 1. 2 · 10 )3 11 2 < 0.05Cysteine 0.7 ± 0 .1 9.6 ... efﬁcient in catalyzing the transamination of anumber of amino acids, including glutamine, phenylalanine,leucine, kynurenine, methionine, tyrosine, histidine, cys-teine, and aminobutyrate (Table 1) ....

Báo cáo khoa học: Cloning, over-expression, puriﬁcation and characterization of Plasmodium falciparum enolase doc

... strain NF54 with P. falciparum strain K1 [17 ] and P. yoelli(NCBI:AA18892) u singCLUSTAL W[24].Enolase from s train N F54 diﬀ ers fro m that of strain K1 in having a P131A mutation (shown in ... strainsdiffer from the K1 strain at position 13 1 in having analanine residue in place of a proline. Figure 1 shows acomparison of amino-acid sequences of enolases fromP. falc iparum strains ... Cys147 and/ or Cys2 41. Analogous positions in Pfen are occupied by Ala2 51 and Cys157. Ala148 replaces Cys157 in Pfen in rabbit muscle enolase. It will be interesting t o examine the effect of thiol-modifying...

Báo cáo khoa học: Systems biology: model based evaluation and comparison of potential explanations for given biological data pot

... example is concerned with insulin signaling, and isinspired by the developments in [12 ]. Insulin signalingoccurs via the insulin receptor (IR). The IR signalingprocesses may be inspected experimentally ... initialmass is in states x 1 and x2 ,1 , the corresponding set of differential equations are:_x 1 ¼Àk 1 x 1 þ 0:001x2 ;1 13 aÞ_x2 ;1 ¼Àk2x2 ;1 þ km 1 x2;mÀ 0:001x2 ;1 13 bÞ_x2;2¼Àk3x2;2þ ... 0:001x2 ;1 13 bÞ_x2;2¼Àk3x2;2þ k2x2 ;1 13 cÞ..._x2;m¼Àkm 1 x2;mþ kmx2;ðm 1 13 dÞy ¼ x 1 13 eÞxð0Þ¼ 10 ; 10 ; 0; 0; Þ 13 fÞHere m is a positive integer, determining the size of the x2subsystem....

Báo cáo khoa học: Sulfated polysaccharides inhibit the catabolism and loss of both large and small proteoglycans in explant cultures of tendon doc

... 4-matrixmetalloproteinase and binding of the activated proteinaseto chondroitin sulfate and heparan sulfate on syndecan- 1. J Biol Chem 279, 10 042 10 0 51. 34 Hausser H & Kresse H (19 91) Binding of heparin and ... FEBSmaintained in DMEM alone. However, in culturesmaintained in DMEM containing 10 0 and 10 00 lgÆmL )1 CaPPS, the percentage of 35S-labeledproteoglycans remaining in the matrix by day 10 of culture ... 16 8DMEM +10 00 lgÆmL )1 CaPPS 18 22 ± 219 12 24 ± 12 9DMEM +10 0 lgÆmL )1 heparin 17 08 ± 3 71 1452 ± 522DMEM +10 00 lgÆmL )1 heparin 14 98 ± 456 13 90 ± 284Table 2. Hexuronate concentration of bovine tendon...

Báo cáo khoa học: Kinetic characterization, structure modelling studies and crystallization of Trypanosoma brucei enolase docx

... J. Inorg. Biochem. 12 , 323–334. 10 . Lee, B.H. & Nowak, T. (19 92) In uence of pH on the Mn2+ activation of and binding to yeast enolase: a functional study.Biochemistry 31, 216 5– 217 1. 11 . ... production of a fusion protein bearingan N-terminal extension of 20 residues including a (His)6-tag and a thrombin cleavage site, leaving three aminoacids (Gly-Ser-His) in front of the initiator ... theoriginal His-tagged protein showed a marked inclination toaggregate upon concentration of 4 mgÆmL )1 (Fig. 5A), in contrast to the thrombin-cleaved protein, for which only% 2% of protein was...

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