... pulse-labeling of the Halo-tagged 17b-HSD11 on the ER, the cellswere exposed to binding dye for 15 min, and were thenwashed and induced for LD formation by changing the culture medium to one containing ... is distributed only in duodenum and jejunumTo examine the distribution of the induced 17b-HSD11 along the gut, proteins from portions of stom-ach, duodenum, jejunum, ileum, cecum and colon of mice ... were fixed in a formaldehyde-basedfixing solution (Genostaff, Inc., Tokyo, Japan) overnight at4 °C, embedded in paraffin, and cut into 6-lm-thick sec-tions. Deparaffinized and rehydrated slides were...