... This kinetic analysis provided new insight
into the role of the linker region. In addition, we fur-
ther analyzed the functional role of the linker region
based on the crystal structures of a ... cleavage of the linker [22]. Although
these results provided valuable suggestions for the role
of the P-gp linker region, the molecular details, such...
... preventing binding of oxygen.
We analyzed the azide inhibition and determined the
inhibition constants for sodium azide, in order to see
the effect of the mutation (L559A) on the binding
properties ... stability of the trinuclear site is
decreased, the binding of azide to the trinuclear site
may be decreased. The depletion of the T2 copper and
chloride ion may...
... base-pairs in the centre of the junctions [19]. Detailed
understanding of the functional role of these interactions
would require the crystal structure of the Ydc2–junction
complex.
In this study the ... [14,20]. Binding of CCE1 results in the
complete unpairing of the four base-pairs in the centre of
the junctions with a lesser disruption of t...
... situated in the E-domain of the fibrin
molecule, the complementary site ‘c’ is suggested to be
situated in the D-domain. We have tried to model the
intermolecular binding between the D-domain of
one ... the N-terminal region
of the Aa-chain by the splitting off of fibrinopeptide A
[6]. Site ‘a’ is formed by amino acid residues cGln329,
cAsp330, cHis340 and cAsp36...
... to DEC1 in the region between amino
acids 48–124 of DEC1. This region, including the bHLH
domain, coincided w ith t he r egion required for bo th the
suppressive activity of DEC1 and the interaction ... Arg65, is responsible for t he
E-box binding.
Determination of the region in BMAL1 for binding
to DEC1
To identify the region in BMAL1 required for the binding...
... obtained by other groups
who also point to the role of the connecting loop of the
b-hairpin of defensins. A combination of mutational
analysis [35] and structural analysis of the plant defensin
Rs-AFP1 ... molecule
comprising Ôresidues in the protruding domain consisting
of the type VI b-turn and the first part of b-strand 3Õ (i.e. the
b-hairpin loop and part...
... this
protease at pH9 [10]. Further interest in the aromatic
stacking is in the role of the electrostatic properties in
enzymatic catalysis of API, and in distinguishing the
functionally catalytic quadruple ... position. In the struc-
tural deviation, the solvent ASA of the side-chain of His210
increased with the decrease in size of the side-chain at
r...
... Pex15p.
To investigate the in uence of individual peroxins of
the importomer on the composition of the membrane-
bound AAA complex, we selected gene deletion strains
affected in the docking complex ... a
control. The corresponding solubilisates
(right panel) were analyzed for the presence
of Pex1p and Pex6p as indicated. The defici-
ency of either protein of the...
... hydrolysis, resulting in an
increase in intracellular sterol trafficking. It is unclear, then,
whether the corresponding increase in pregnenolone pro-
duction results from an increase in free sterol ... ([14,15]). In the latter case, the
concentration of Adrp limits the production of pregneno-
lone [15] through a mechanism involving oxidized Adxp,
that is in excess in...
... anal-
ysis of plant b-glucosidases to obtain insights into the
evolution of the molecular sites involved in the two
modes of aglycone binding.
The amino acid sequence of Zm-p60.1 was com-
pared with the ... distances of reacting
atoms. The only noted difference was in the orienta-
tion of the aromatic ring of pNPGlc in the F193A
mutant (Fig. 6D), owing to...