Báo cáo khoa học: Solubility-dependent structural formation of a 25-residue, natively unfolded protein, induced by addition of a seven-residue peptide fragment pot

... formation of a 25-residue, natively unfolded protein, induced by addition of a seven-residue peptide fragment Mitsugu Araki and Atsuo TamuraGraduate School of Science, Kobe University, Nada, JapanIn ... 7801–7809.15 Araki M & Tamura A (2007) Transformation of analpha-helix peptide into a beta-hairpin induced by addi-tion of a fragment results in creation of a coexistingstate. Proteins ... YAFACPACPK RFMRSDALSK HIKTAFIVVA30LGFP2 YAFACPACPK RFMRSDALSK HIKTAYIVVA LGFP3 YAFACPACPK RFMRSDALSK HIKTAYISVA LGM. Araki and A. Tamura Solubility-dependent structure formation FEBS Journal 276 (2009)...

Tài liệu Báo cáo khoa học: NMR structural characterization of HIV-1 virus protein U cytoplasmic domain in the presence of dodecylphosphatidylcholine micelles doc

... the aqueous buffer. Qualitative analysis of secondary chemicalshift and paramagnetic relaxation enhancement data in conjunction withdynamic information from heteronuclear NOEs and structural ... the calculated structures was acceptable; 89% of the analysed backbone torsions fell into the mostfavoured and additionally allowed regions of the Rama-chandran plot. An additional 6% of residues ... structure of micelle-associated VpUcyt was cal-culated on the basis of the ﬁnal set of proton–proton dis-tance constraints using cyana, a software program thatcombines simulated annealing with...

Tài liệu Báo cáo khoa học: Novel aggregate formation of a frame-shift mutant protein of tissue-nonspeciﬁc alkaline phosphatase is ascribed to three cysteine residues in the C-terminal extension pdf

... Michigami T, Yamamoto T, Yasui N, SatomuraK, Yamagata M, Shima M, Nakajima S, Mushiake S,Okada S & Ozono K (1998) Analysis of localization of mutated tissue-nonspeciﬁc alkaline phosphatase ... fraction).BSA (b, 68 kDa), alcohol dehydrogenase (a, 141 kDa) and catalase (250 kDa) wereloaded on to a separate gradient as mole-cular mass markers.K. Komaru et al. Novel aggregate formation of an alkaline ... School of Medical and Dental Sciences, Gakkocho-dori, Niigata, Japan2 Kitasato Junior College of Health and Hygienic Sciences, Yamatomachi, Minami-Uonuma-shi, Niigata, Japan3 Department of Food and...

Báo cáo khoa học: Amyloid oligomers: formation and toxicity of Ab oligomers ppt

... Saitama, Japan2 PRESTO, Japan Science and Technology Agency, Kawaguchi, Saitama, JapanIntroductionAlzheimer’s disease (AD) is an age-related, progressivedegenerative disorder characterized by ... via a pathway distinct from that of fibrilFig. 1. Formation and toxicity mechanisms of extracellular Ab oligomers. Ab is released extracellularly as a product of proteolytically cleaved,plasma ... multiple pathways(Fig. 1).Intracellular AbAlthough Ab was first identified as a component of extracellular amyloid plaques, ample evidence hasdemonstrated that Ab is also generated intracellularly(reviewed...

Báo cáo khoa học: The structural basis for catalytic function of GMD and RMD, two closely related enzymes from the GDP-D-rhamnose biosynthesis pathway pdf

... thermoaerophiluswere ampliﬁed from chromosomal DNA templates, using thefollowing primer pairs: P. aeruginosa,5¢-AGGCCCGCTTCCGGATCCACTCAGCGTCTG-3¢ and 5¢-AAAAAAGTCGACTTCTTCTCGTACCCGTGACTC-3¢; and A. thermo-aerophilus,5¢-TTGGATCCATGAGAGCCCTAATCACTGGA-3¢ ... collected at a wavelength of 1.0 A ˚on a MAR CCD detector at the Advanced PhotonSource Beamline 5-ID (DND) (Argonne National Labora-tory). Crystals were held at 100 K in a cryostream duringdata collection. ... of Innovation and the Ontario Innovation Trust.References1 Kocharova NA, Knirel YA, Widmalm G, Jansson PE& Moran AP (2000) Structure of an atypical O-antigenpolysaccharide of Helicobacter...

Báo cáo khoa học: Ternary complex formation of pVHL, elongin B and elongin C visualized in living cells by a ﬂuorescence resonance energy transfer–ﬂuorescence lifetime imaging microscopy technique docx

... 5¢-CTAGAGTTAACCCGGGATATCCAGGTCTTCCTC ACTGATCAGCTTCTGTTCCTCCATGGTGGT-3¢, and 5¢-CTAGACCACCATGGACTACAAAGACGATGACGATAAAGATATCCCGGGTTAACT-3¢ and 5¢-CTAGAGTTAACCCGGGATATCTTTATCGTC ATCGTCTTT GTAGTCC ... the annealed fragment of the synthesized oligonucleotides, 5¢-CTAGACCACCATGTACCCCTACGACGTGCCCGACTACGCCGATATCCCGGGTTAACT-3¢ and 5¢-CTAGAGTTAACCCGGGATATCGGCGTAGTCGGGCACGTCGTAGGGGTACATGGTGGT-3¢, ... XbaI site of pBOS Vector.pBOS-Myc and pBOSFlag were constructed similarly by using the synthesized oligonucleotides 5¢-CTAGACCACCATGGAGGAACAGAAGCTGATCAGTGAGGAAGACCTGGATATCCCGGGTTAACT-3¢ and...

Báo cáo khoa học: Nitric oxide formation from the reaction of nitrite with carp and rabbit hemoglobin at intermediate oxygen saturations pdf

... oxygenated carpHb at 100% So2was clearly autocatalytic. The reac-tion rate initially showed a sharp increase, reached a marked peak and then displayed a decrease, as thereaction approached ... small amounts and disappeared com-pletely when the reaction entered the autocatalyticphase (Fig. 3D).Reaction in presence of ATPThe addition of ATP to carp Hb at an [ATP] ⁄ [Hb]ratio of ... present at 48% So2and above [20]. A similar situ-ation was found in rabbit Hb, where autocatalysis wasabsent at 46% So2but present at 67% So2(Fig. 3). Incarp Hb, autocatalysis was absent at...

Báo cáo khoa học: The structural basis of calpain behavior pptx

... (l-cal-pain or calpain 1) and milli-calpain (m-calpain orcalpain 2), have been the focus of three decades of intensive characterization. In vitro analysis has shownthat the Ca2+concentration ... stars). ThisCa2+-free conﬁguration corresponds to a structurallyinactive conformation of calpain 2. DIII is related tothe spatial organization of the C2 domain and bindsCa2+ions and phospholipids. ... optimalactivity is 5–50 lm for calpain 1 and 0.2–1 mm for cal-pain 2. A large range of substrates and a commoninhibitor (calpastatin), also found in the nucleus, wereidentiﬁed. Knowledge of...

Báo cáo khoa học: The structural comparison of the bacterial PepX and human DPP-IV reveals sites for the design of inhibitors of PepX activity pot

... againstthe X-PDAP of pathogens would probably affect theactivity of the mammalian enzymes, leading to harmfulconsequences for human health. Taking advantage of the structural differences and ... 1.70 a Tests carried out on DPP-IV to validate the computational protocol of AUTODOCK.bMean of values calculated by AUTODOCK 3.0.cMean of the rmsd calculated between the solutions of AUTODOCK ... speciﬁc activity andinhibition studiesH-Ala-Pro-para-nitroanilide (Ala-Pro-pNA) was purchasedfrom Sigma (St Louis, MO, USA) and valine-pyrrolidide wassynthesized by Neosystem (Strasbourg, France)....

Tài liệu Báo cáo khoa học: Activation loop 3 and the 170 loop interact in the active conformation of coagulation factor VIIa pdf

... precisely, anFig. 4. N-terminal pegylation of G37 2A- FVIIa and FVIIa. (A) Pegyla-tion of free and TF-bound G37 2A- FVIIa and FVIIa visualized by SDS–PAGE. At each time point, a 12 lL aliquot of the reaction ... (Protein Data Bankaccession number 1j 8a) with Asn in this position, albeitwith F,W angles far from the allowed Ramachandranregion, are virtually identical to that of FVIIa. Model-ling of Ala372(223) ... procoagulant activity that triggers theclotting cascade [1,2]. Importantly, formation of thebinary complex localizes FVIIa to the site of vasculardamage, positions the active site at an appropriatedistance...

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