Báo cáo khoa học: Full-length adiponectin protects hepatocytes from palmitate-induced apoptosis via inhibition of c-Jun NH2 terminal kinase doc

Báo cáo khoa học: MicroRNA-21 protects neurons from ischemic death pot

... ischemic kidney, and showed that knockdown of Faslg protects renal cells from apoptosis, and, infact, animals from death. We speculate that overex-pression of miR-21 may represent a way to usean ... used the TUNEL assay tomeasure the proportion of cells undergoing apoptosis. Inhibition of miR-21 in neurons signiﬁcantly increasedthe number of TUNEL-positive cells after OGD ascompared with ... upregu-lation of miR-21 in neurons.miR-21 protects cultured cortical neurons in vitroTo investigate the dynamics of miR-21 in vitro,weemployed a model of ischemia in which primarycortical neurons from...

Tài liệu Báo cáo khoa học: Brain angiogenesis in developmental and pathological processes: therapeutic aspects of vascular endothelial growth factor doc

... ischemic and stillviable region of the brain called the ‘penumbra’ [14].Therefore, degree of angiogenesis appears to correlatewith rate of recovery from stroke.A variety of angiogenic factors ... biochemically.The afﬁnity of VEGFR-1 for VEGF-A is extremelyhigh (Kd= 1–10 pm), 10-fold that of VEGFR-2.However, the tyrosine kinase activity of VEGFR-1 isone order of magnitude lower than that of VEGFR-2which ... 17014020 from theMinistry of Education, Culture, Sports, Science andTechnology of Japan, a grant from the program‘Research for the Future’ of the Japan Society for thePromotion of Science,...

Tài liệu Báo cáo khoa học: Autophagy inhibits reactive oxygen species-mediated apoptosis via activating p38-nuclear factor-kappa B survival pathways in oridonin-treated murine ﬁbrosarcoma L929 cells doc

... anti-apoptoticmechanism of NF-jB have demonstrated that the acti-vation of NF-jB impaired c-Jun N -terminal kinase (JNK) activation [23], or resulted in an increase of Bcl-2 family protein levels ... negative regulator of apoptosis, whereas ERK contributed to apoptosis. Furthermore, ROS might be involved in theactivation of ERK pathway in oridonin-induced L929cells. Inhibition of NF-jB and p38 ... understanding of moresigniﬁcant molecular mechanisms between apoptosis and autophagy.Experimental proceduresReagentsOridonin was obtained from the Kunming Institute of Botany, Chinese Academy of Sciences...

Báo cáo khoa học: Nuclear actin and actin-binding proteins in the regulation of transcription and gene expression docx

... STARS stimulates MRTFactivity by inducing the dissociation of MRTFs from actin via depletion of the G-actin pool.The N -terminal regions of MRTFs contain threeRPEL motifs which have been shown ... C -terminal domain of Pol II [43]. Injection of a peptide acting as a competitive inhibitor of pro-tein–protein contact involving actin and the hnRNPprotein, HRP36, into the salivary glands of ... Solution structures of theC -terminal headpiece subdomains of human villin andadvillin, evaluation of headpiece F-actin-bindingrequirements. Protein Sci 13, 1276–1287.60 Roof DJ, Hayes A, Adamian...

Báo cáo khoa học: The essential tyrosine-containing loop conformation and the role of the C-terminal multi-helix region in eukaryotic phenylalanine ammonia-lyases docx

... regulation of eukaryotic PALsAlthough the regulation of eukaryotic PALs differs,the necessity of rapid inactivation ⁄ decomposition of the enzyme as well as the presence of the C -terminal multi-helix ... threeisoenzymes of PAL (PAL1, PAL2 and PAL3) areknown [76]. After the final chromatofocusing stage of PAL purification from suspension-cultured cells of French bean, four forms of the enzyme with ... the Hill-coefficientshould indicate significant deviation from 1.0 (negativecooperation).Kinetic properties of PAL from various sourcesPAL from different sources exhibits considerable vari-ation...

Báo cáo khoa học: UDP-galactose 4-epimerase from Kluyveromyces fragilis – catalytic sites of the homodimeric enzyme are functional and regulated docx

... inhibi-tion of epimerase offered a sensitive method for deter-mination of stoichiometry of the bound cofactor.Reductive inhibition was applied to 33.3 nmol (5 mg) of epimerase. A control set of the ... basis of bind-ing with the inhibitor. Careful analysis of Fig. 2 showsthat inactivation of E3by trypsin and reductive inhibi-tion consistently deviated from 50%, in spite of modi-ﬁcation of ... the mutarotase activity of the C -terminal part of epimerase remains unaltered after addition of the highest concentration of 5¢-UMP as applied here[11]. The signiﬁcance of dimerization may rest...

Báo cáo khoa học: Examining multiprotein signaling complexes from all angles The use of complementary techniques to characterize complex formation at the adapter protein, linker for activation of T cells pdf

... Inconfirmation of the role of these LAT tyrosines onCa2+influx and the activation of MAP kinases, eithermutation of LAT tyrosine 132 or LAT tyrosines 171,J. C. D. Houtman et al. Investigation of multiprotein ... composition of multiprotein complexes are detailed. Also described is the information gained from these techniques and examples of the use of these techniques for the examination of linker for ... thetransfer of energy from an excited donor fluorophoredirectly to an acceptor fluorophore, leading to anincreased fluorescence emission of the acceptor and aquenching of the emission fluorescence of the...

Báo cáo khoa học: Escherichia coli cyclophilin B binds a highly distorted form of trans-prolyl peptide isomer doc

... between NH of Ala88 and CO of Gly72 of human CyPA with a distance of 2.84 A˚. T he ﬁxing byhydrogen bonds of Ala88 and Pro90 to CyPA generates thedeviation of x angle of  20° from the plane ... b3strand, but the N atom of Pro2 is 4.02 A˚away from theNg2atom of Arg43. T he Ng1atom of Arg43 i s not within5.5 A˚ from the N atom of Pro2. In addition, NH and CO of Ala1 of the cis-form have ... of Ca, C and O of Gly89 has the rotation angle x of  20° from the plane of N, Ca and Cd of Pro90 around theGly-Pro amide bond. The //w torsional angles for Pro90are )78/141° and the ring of...

Tài liệu Báo cáo khoa học: Chromatin under mechanical stress: from single 30 nm ﬁbers to single nucleosomes pdf

... in Prague, First Faculty of Medicine, Institute of Cellular Biology and Pathology, Prague, Czech Republic3 Department of Cell Biology, Institute of Physiology, Academy of Science, Prague, Czech ... is composed of an octamer of core histones (two each of H2A, H2B, H3 and H4), alinker histone and  166 bp ( 56 nm) of DNA [6].The histone octamer alone associates with 146 bp of DNA ( 50 ... (instead of 76 bp in [41]) forcontinuous release of the outer DNA turn and 72 bp(instead of 82 bp) for disruption of the inner turn. Inall studied cases the removal or modiﬁcation of histonetails...

Tài liệu Báo cáo khoa học: EGF receptor in relation to tumor development: molecular basis of responsiveness of cancer cells to EGFR-targeting tyrosine kinase inhibitors docx

... tyrosine kinase inhibitor-induced apoptosis via c-Jun NH2 -terminal kinase and p38mitogen-activated protein kinase. AbbreviationsBH, Bcl-2 homology domain; Bim, Bcl-2 interacting mediator of cell ... IAP, inhibitor of apoptosis protein; JNK, c-Jun NH2 -terminal kinase; KIP, kinase inhibitor proteins; MEK,MAPK ⁄ ERK kinase; MKK, MAPK kinase; MKP-1, mitogen-activated protein kinase phosphatase-1; ... causes apoptosis. TheT790M mutation of the EGFR, which renders geﬁti-nib and erlotinib ineffective inhibitors of EGFR kinase activity, blocks geﬁtinb-induced up-regulation of Bim and apoptosis...

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