Báo cáo khoa học: Analysis of the RNA degradosome complex in Vibrio angustum S14 potx
Báo cáo khoa học: Analysis of the RNA degradosome complex in Vibrio angustum S14 potx
... al. RNA degradosome complex in Vibrio angustum S14 FEBS Journal 277 (2010) 5161–5173 ª 2010 The Authors Journal compilation ª 2010 FEBS 5173 Analysis of the RNA degradosome complex in Vibrio angustum ... itsrole in mRNA degradation, the degradosome is alsoresponsible for the processing of 5S ribosomal RNA and tRNAs, as well as the degradation of tmRNAs [4–8]. The principal proteins comprising the degradosome include ... for the V. angustum S14 RNA degradosome. We discuss the benefits of using Blue Native-PAGE as a toolto analyse the RNA degradosome, and the implications of microdomain-mediated RNase E interaction...
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Tài liệu Báo cáo khoa học: Analysis of the molecular dynamics of medaka nuage proteins by fluorescence correlation spectroscopy and fluorescence recovery after photobleaching doc
... mesoderm 0 min 1 min 2 min 3 min 4 min 9 min BAFig. 2. Time-lapse LSM analysis of Olvas–GFP reveals the dynamicnature of the nuage. Schematic diagram of the preparation of PGCs of medaka specimen ... diagram of the constructs microinjected into the medaka eggs and transfected into HeLa cells. The olvas, nanos and tudorcoding sequences were joined to the C-terminal region of the GFP or RFP coding ... outside of the nuage in the cytoplasm of the migrating PGC. FCS analysis revealed that Olvas–GFP diffused with a diffusion constant of 7.0 lm2Æs)1(Fig. 3A). During the measurement of the PGC, the olvas3'UTRGFP/RFP...
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Báo cáo khoa học: Analysis of Lsm1p and Lsm8p domains in the cellular localization of Lsm complexes in budding yeast ppt
... byaffecting the RNA- binding ability of the resultingheteroheptameric Lsm complex (see Lsm8DC, Lsm818,Lsm881 and Lsm188). Minimal U6 binding byGFPGFPIPInputLsm7-MycU6 RNA U4 RNA U4 RNA U6 RNA scR1GFP-LSM8GFP-lsm8 ... mutational analysis of Lsm1p showing the importance of residuesproposed to be involved in RNA binding and complex formation, and of the C-terminal region for the func-tional competence of the Lsm1–7p ... thosewith the C-terminal domain deleted is consistentwith this (Figs 4 and 5). Hybrid proteins carrying the N-terminus of one protein and the Sm domain of the other localize according to the N-terminal...
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Báo cáo khoa học: Analysis of the NADH-dependent retinaldehyde reductase activity of amphioxus retinol dehydrogenase enzymes enhances our understanding of the evolution of the retinol dehydrogenase family pot
... [29]. The overall analysis, examining the topology of the phylogenetic tree and the position of each gene inside the cluster, was informative regard-ing the orthology relationships of the distinct ... dashes. The active site (YTVAK) and the cofactor-binding motifs aremarked in bold. The D43, T67 and A69 residues, involved in cofactor specificity, are indicated by asterisks. Flanking the N-terminal ... on these two protein families, the majorenzyme(s) responsible for the in vivo oxidation of retinolremains uncertain. In previous studies, we analyzed the functionality and evolution of the...
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Báo cáo khoa học: Analysis of the regulatory motifs in eukaryotic initiation factor 4E-binding protein 1 pot
... define further the features in the C-ter-minus of 4E-BP1 that are involved in its binding toraptor. The data for the other truncation mutantsshown in Fig. 1C indicate that other regions of 4E-BP1 ... after treatment of cells with insulin[25]. Replacing its N-terminus with the N-terminal part of 4E-BP1 led to a marked increase in its phosphoryla-tion (even in the absence of insulin). Thus, it ... 2A). The binding of raptor to these two variants was similar (Fig. 1D),implying that the loss of raptor binding on removal of the first 17 residues may be accounted for simply by the loss of the...
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Báo cáo khoa học: Analysis of the transcarbamoylation-dehydration reaction catalyzed by the hydrogenase maturation proteins HypF and HypE pot
... the maturation of the enzymes. Most o f them a ct pleiotropically in the synthesis of all four hydrogenases, in particular in the synthesis and insertion of the metal centre [6,7]. Two of the products of the ... transferring the carboxamido moiety in anATP-dependent reaction to the thiolate of the C-terminalcysteine of the HypE protein yielding a protein-S-carbox-amide [10–12]. Subsequent dehydration of the ... HypE-thiocyanate involves first the carbamoylation of the C -terminal c ysteine of HypE b yinteraction with HypF, then the release of HypF and the subsequent dehydration of the protein th iocarboxamide...
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Báo cáo khoa học: Analysis of the in planta antiviral activity of elderberry ribosome-inactivating proteins potx
... confirm the presence of the RIP/lectin codingsequence in the tobacco genome. Only those plants thatwere positive in the PCR analysis were withheld for further analysis. Expression of the RIP/lectin ... Therefore, the presence of mRNAs encodingPR-1, PR-2, PR-3 and proteinase inhibitor II was verifiedby Northern blot analysis. In none of the transgenic plantscould any of these mRNAs be detected ... between in planta antiviral activity of transgenic tobacco lines expressing the different ribosome-inactivating proteins and the in vitro polynucleotide–adenosine glycosylase activity of the respective...
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Báo cáo khoa học: Analysis of the stability of the spermadhesin PSP-I ⁄ PSP-II heterodimer Effects of Zn 2+ and acidic pH pdf
... accommodated within the hydrophobic core of the CUB domain. This coreis conserved in the X-ray structures of proteins con-taining the CUB signature, including the mannan-binding lectin-associated ... [11,21,22]. These binding sites are nonethelesscryptic in the heterodimer, which is typically isolatedfrom the nonheparin-binding fraction of boar seminalplasma [11], raising the question of their ... Dimerizationmarkedly affects the binding ability of the subunits. Notably, heparin andmannose 6-phosphate binding abilities of PSP-II are abolished, indicatingthat the corresponding binding sites may be...
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Báo cáo khoa học: Analysis of the effect of potato carboxypeptidase inhibitor pro-sequence on the folding of the mature protein pot
... different protein variants, we have investi-gated the role of both pro-sequences in the in vitro refolding of PCI, together with studies of the in uence of the N-terminal pro-region on its in vivo ... extremelyinefficient folding of PCI in vitro [18], together with the presence of the above-mentioned long pro-sequences,suggest a possible involvement of them in the in vivo folding of PCI.Here, using different ... during 2 min; then 10 lLofincreasingconcentrations of PCI or ProNtPCI were added and the measures were continued for 2 min. The slope of the first part of the assay corresponded to moand the...
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Báo cáo khoa học: Analysis of the region for receptor binding and triggering of oligomerization on Bacillus thuringiensis Cry1Aa toxin potx
... (a) the con-struction of cysteine-substituted mutant toxins and the analysis of their binding activity to the receptor toinvestigate the role of mutated amino acid residues; (b) the analysis of ... Mapping of the mutation site. Red indicates the amino acidresidues that showed inhibition of BtR175 binding by DF binding.Yellow indicates the amino acid residues that showed inhibition of BtR175 ... ablated.Binding analysis of DF-bound toxins andIAF-modified toxinsRegardless of whether the toxin does or does not oli-gomerize, modification at cysteine residues can affect the binding affinity of the...
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