... Water and Environment Technology, Vol. 8, No.4, 2010 - 300 - produced another brood, and nauplii hatched from these females two times, i.e. from the first brood on Day 2-4 and from the second ... study, the acute and subchronic toxicities of TBTCl to T. japonicus were presented. The acute toxicity was expressed as the LC50 and highest NOLC and the subchronic toxicity, which focused on the ... Substances in 1990 under the “Law Concerning the Examination and Regulation of Manufacture, etc. of Chemical Substances” in Japan. The toxicity level of TBTCl may be as high as that of tributyltin oxide...