... DosH as a template and using the following respective 5¢-sense primers: 5¢-gatgagtcgggagACCcagctggagaaaaaag-3¢,5¢-gatgagtcgggagTTTcagctggagaaaaaag-3¢,5¢-ggacccgttttgcgACCtcgaaagtgagc-3¢, and ... examined theirphysicochemical characteristics, including auto-oxidation rates, O2 and CObinding kinetics, and redox potentials. The Fe(III) complex of the L115Fmutant was unstable and had a ... hydrophobic characteristic of the heme distal sidecontributes substantially to the ligand binding kinetics of the PAS domain of Ec DOS, as it does in myoglo-bin and hemoglobin. Examination of the effects...