Báo cáo khoa học: Enhanced sensitivity to hydrogen peroxide-induced apoptosis in Evi1 transformed Rat1 fibroblasts due to repression of carbonic anhydrase III docx
... Journal compilation ª 2009 FEBS
Enhanced sensitivity to hydrogen peroxide-induced
apoptosis in Evi1 transformed Rat1 fibroblasts due to
repression of carbonic anhydrase III
P. Roy
1
, E. Reavey
1
, ... H
2
O
2
-induced
apoptosis in Rat1 cells expressing an Evi1 transgene.
Surprisingly, we found that Evi1 expression increases
sensitivity to H...
... of the enzyme [7]. Having previously cloned the
mph gene from Ochrobactrum sp. M231 [7], we sought
to increase the thermostability of this MPH using pro-
tein engineering.
The two main protein-engineering ... September
2010)
doi:10.1111/j.1742-4658.2010.07895.x
Protein thermostability can be increased by some glycine to proline muta-
tions in a target protein. However, not all glyci...
... expression of G
L
and PTG, but not
R6, in rat liver in vivo is insulin-dependent. It declines
during insulin deficiency and is restored by insulin
treatment [22,40]. Another glycogen-targeting protein
expressed ... glucose-
induced insulin secretion and insulin resistance in the
liver and periphery. Hepatic insulin resistance is attrib-
uted to a range of metabolic defects, which i...
... release zinc from zinc-binding proteins
The effect of aldehydes on the zinc-binding capacity of
zinc proteins was assayed by employing spectropho-
tometry and the chromophoric indicator 4-(2-pyridyl-
azo)-resorcinol ... cysteine ligands of zinc sites in proteins
and concomitant zinc release. Stoichiometric amounts of acrolein release
zinc from zinc–thiolate coordination sites in p...
... each dataset into 10 subsets
where each subset was a test set and the corre-
sponding 9 remaining sets were merged to a train-
ing set. We kept the labels of the training set
to determine model ... our
experimental setup. Instead of dividing datasets
during 10-fold cross-validation into training and
test subsets with the ratio of 9:1 we randomly split
the data into training, validat...
... eosin staining. We examined the expres-
sion of these proteins in a cancer-free layer of squamous
epithelial cells, carcinoma in situ (CIS) and invasive cancer,
all of which were observed in ... proteins bound to chromatin probably
function in DNA replication, but the role of the proteins not
tightly bound to chromatin remains to be determined.
Consistent with the result...
... amino acid homocysteine both in vitro
and in vivo. AS101 conferred protection against homocysteine-induced
apoptosis of HL-60 cells. The protective mechanism of AS101 against
homocysteine toxicity ... homocysteine to form homocystine, the less
toxic disulfide form of homocysteine. Moreover, AS101 was shown here to
reduce the levels of total homocysteine in an in vivo mode...
... deubiquitinating enzymes, mainly consisting
of cysteine proteases [43]. Another class of proteins are the
tail-anchored proteins, which include the translocases of the
mitochondrial outer membrane, Tom5, ... Biochemicals,
Stockton, UK) as substrate, 20 lL yeast extract, various
inhibitors and activators in a total volume of 30 lL. The
mixture was incubated at 30 °C f or 2.5 min to...
... that single-chain HGF
was inactive in the presence of serine protease inhibi-
tors in a hepatocyte proliferation assay, whereas the
two-chain form was active even in the presence of the
inhibitors ... next
administered hepatotoxin or nephrotoxin to rats to
induce tissue injury. HGF was extracted from injured
and uninjured tissues and then analyzed. After intraga-
stric administr...
... with an increase in
the levels of several FAA during the first days in most
species tested to date, resulting in an increase of the
total FAA pool [13,19]. Even though no significant
increase of the ... adults of the beetle Alphitobius
diaperinus in order to determine whether FTRs trigger the initiation of a
metabolic response involving synthesis of protective compounds,...