Báo cáo khoa học: Nuclear factor kappa B and tumor necrosis factor-alpha modulation of transcription of the mouse testis- and pre-implantation development-specific Rnf33⁄Trim60 gene pot
... located in the single intron of the Rnf33 gene
adjacent to exon 1; the intronic jB was targeted by
the p65 ⁄ RelA and p50 ⁄ NFjB1 nuclear < /b> factor-< /b> jB
(NF-jB) subunits, and the jB-dependent transcrip-
tion ... FEBS
Nuclear < /b> factor < /b> kappa < /b> B and tumor necrosis factor-< /b> alpha
modulation of transcription of the mouse testi...
... groove binder (MGB) probe, let- 7b
5¢-S-carboxyfluorescein (FAM)-agtgatgttgcccc-MGB 3¢, was
designed to have a 5¢ FAM and an MGB at the 3¢ end.
The TaqMan MGB probe was synthesized by Applied Bio-
systems. ... transfection of these constructs. In this type of experiment, the level of the interac-
tion between the endogenous let- 7b and miR-663 and the expression vecto...
... AES situation that they
are not suitable for the characteristic.
The impact of the first problem can be reduced
by either perfecting the results of the ASR system or
building the AES system which ... experiments will be done in
both transcriptions. A better understanding of the
difference in the CRR transcription and the ASR
transcription from the low score to...
... for
mesothelioma [1].
The first step in RNase cytotoxicity is the internalization
of the protein across the phospholipid bilayer barrier.
a-Sarcin, the best characterized member of the family of
fungal ... inhibit protein synthesis and eventually
cause cell death [3,4], while the other is composed of certain
members of the bovine pancreatic RNase A superfamily.
Onco...
... al. (Eur. J. Biochem. 271) Ó FEBS 2004
of the sponge cell to the gene expression level and back to a
further molecule potentially involved in the recognition of
the carbohydrate, the fibrinogen-like ... tridentatus
[20] (AB024737.1 and AB024738.1), and the angiopoietins
from mammals, e.g. humans (O15123), form the second
branch. The third branch is built by the fic...
... FEBS
AaHR3
0
500
1000
1500
2000
2500
b
ab
a
a
b
b
b
b
AaHR4
0
300
1000
1500
2000
2500
a
b
b
b
b
b
b
b
AaE78
50
100
150
200
250
300
a
a
a
a
a
a
a
a
0
100
200
AaEcR-A
0
20
40
60
80
100
120
b
a
b
b
b
b
b
b
0 h 6 h 12 h ... (+SEM)
AaUSP-A
0
10
20
30
40
50
60
a
b
b
b
b
b
b
b
AaUSP -B
0
5
10
15
20
25
a
a
a
a
a
a
a
a
AaE75A
1 d 4 d 6 h 12 h 24 h 36 h 48 h 72 h
0
400
80...
... smeared bands (bands 1 and
2) with molecular masses of 20–30 kDa observed on
SDS ⁄ PAGE. Subsequently, the level of the approxi-
mately 15-kDa band increased, and TFPI (43 kDa) as
well as bands 1 and ... resi-
dues. On the basis of these observations, we believe
that the recombinant TFPI carried an O-linked carbo-
hydrate chain on Thr14. Moreover, the peak heights
and...
... angiogenic factors, in cluding fibroblast
growth factors (FGFs), platelet-derived growth factor < /b> B
(PDGF -B) and vascular endothelial growth factor < /b> (VEGF)
[1,2]. These factors are counterbalanced by inhibitory
molecules ... and compared the mechanisms by
which PF-4 i nhibits the DNA synthesis induced by
VEGF
165
and VEGF
121
.
The DNA synthesis induced by VEGF
165
a...
... expression of PKB was calculated by the intensity
ratio of PKB band and b- actin band.
Assay of PKB activity
PKB assay was performed with a widely used assay kit of
Akt kinase according to the instruction ... added, and the incubation
time was extended to 36 h. Then the cells were fixed, stained
and countered as described above, and the number of cells
that had mig...
... disrupts
interactions between the N- and C-termini of the AR,
and interferes with the binding of the coactivator TIF2
[86,91]. There is evidence that interaction between the
FXXLF (X = any amino acid) motif of the ... actin and actin-binding proteins in the regulation
of transcription and gene expression
Bin Zheng
1
, Mei Han
1
, Michel Bernier
2
and Jin-ku...