Báo cáo khoa học: TNFR1-induced activation of the classical NF-jB pathway Harald Wajant1 and Peter Scheurich2 pptx

... at the edge of the --Fig. 1. Activation of classical and alternative NF-jB signaling. The classical NF-jB pathway can be activated by a broad range of stimuli,including most ligands of the ... [1,2]. The twomechanisms of NF-jB dimer inhibition correspond to the existence of two different NF-jB- activating path-ways called the classical and the alternative NF-jB pathway (Fig. 1). The classical ... released in the cytoplasm and can nowtranslocate into the nucleus. Activation of the alterna-tive NF-jB pathway is independent of IKK2 and NEMO and requires degradation of TRAF proteins and subsequent...

Báo cáo khoa học: Biochemical properties of the human guanylate binding protein 5 and a tumor-speciﬁc truncated splice variant Mark Wehner and Christian Herrmann doc

... coefﬁcients of 2.4 and 1.9 and dissoci-ation constants (KdHill) of 4 and 2 lm for hGBP5a ⁄ b and hGBP5ta, respectively. The proteins are very similarin terms of their enzymatic activity, so the ... manner, the entropic contributions of nucleotide binding and the coupled oligomerizationshow opposing signs. In the case of hGBP1, release of water from the nucleotide binding pocket and the pro-tein ... byhGBP5, and the enzymatic hydrolysis of GTP leads toGDP rather than GMP. The nucleotide afﬁnities of hGBP5 are grossly similar to those of hGBP1, butthere are differences in thermodynamic and kineticdetails....

Báo cáo khoa học: CpG methylation of the CENP-B box reduces human CENP-B binding pptx

... CENP-B and the unmethylated CENP-B box DNA at sites1 and 3. The pink dotted line in the right panel indicates the hydrogen bond between the side chain NH2group of Arg125 and the N7 atom of guanine.Y. ... and the N7 atom of guanine 16 by the CpGmethylation may be a reason for the reduced speciﬁcity of CENP-B to the CENP-B box sequence.What is the functional meaning of the CpG methyla-tion of ... lanes 1–6, and B). On the other hand, the complexbetween CENP-B(1–129) and the 32P-labeled methyla-ted CENP-B box DNA was signiﬁcantly dissociated in the presence of an equal amount of unlabeled...

Tài liệu Báo cáo khoa học: Quantitative analysis of the experimental O–J–I–P chlorophyll ﬂuorescence induction kinetics Apparent activation energy and origin of each kinetic step Steve Boisvert, David Joly and Robert Carpentier doc

... depends on the relative amplitude and rate constant of the J–Iphase compared to the amplitudes and rate constants of the two other phases. The above should be gov-erned by the balance between the ... when the temperature is raised. The effect of DCMU on the traces was to increase the amplitude of step J with the concurrent decline of stepI, and to retard the rise to Fm. With dPQ, the J ... amplitude and half-time of the J–I phase were bothdecreased with DCMU, demonstrating that the J–I riseis not directly related to the reduction of the PQ pool.A further analysis of the inﬂuence of...

Báo cáo khoa học: Lid L11 of the glutamine amidotransferase domain of CTP synthase mediates allosteric GTP activation of glutaminase activity docx

... analysis of the structure of the T. thermophilus enzyme [15], it is suggested that the binding of ATP and UTP to the enzyme bringstogether the GATase and the synthase domains and enables the formation ... glutamine hydrolysis and GTP activation of thisreaction. The uncoupled glutaminase activity of boththese mutant enzymes no longer responds to the pres-ence of GTP (Table 2) and the activation mediated ... is greatly stimulatedby the allosteric binding of GTP and this activation has been the focus of several reports [5–9]. CTP syn-thase is the only member of the family of GATasedomain harboring...

Báo cáo khoa học: Alanine screening of the intracellular loops of the human bradykinin B2 receptor – effects on receptor maintenance, G protein activation and internalization pdf

... the respective construct name next to them. The pointmutations E66A in the ﬁrst loop and T242A at the end of the third loop are also indicated. The two-dimensional structure of B2R, with the membrane ... expres-sion of the constructs under the control of the CMV promoter; ﬁlledcolumns, HA-tagged constructs under the control of the weakerPminpromoter. (B) Immunoblot of B2Rwt, mutant 2 ⁄ 1 and the ... throughchanges in the overall structure of the receptor. Thus,these data demonstrate the connection between the structure of ICLs and that of the binding site, implyingreciprocally that changes at the binding...

Báo cáo khoa học: Catalytic activation of human glucokinase by substrate binding – residue contacts involved in the binding of D-glucose to the super-open form and conformational transitions ppt

... identify the activesite residues involved in the binding of Glc to the super-open state (Fig. 1C) of this two domain [large(L) and small (S)] enzyme, and thus the site of initia-tion of the global ... [27–29].Two of these (Y214C and Y215A) are localized inhelix 6 and three (V455M, A456V and A460R) inhelix 17, and some of these missense mutations (e.g.Y215A and V455M) perturb the hydrophobic ... binding of Glc based on the coordi-nates of the unliganded and the liganded form. Helix–helixinteractions in the two conformational states were analysedas described previously [35]. The static...

Báo cáo khoa học: "Quantitative modeling of the neural representation of adjective-noun phrases to account for fMRI activation" doc

... model and the multplicative model. The adjective model assumes that the meaning of the composition is the same as the adjective: up= The noun model assumes that the meaning of the ... Proceedings of the 47th Annual Meeting of the ACL and the 4th IJCNLP of the AFNLP, pages 638–646,Suntec, Singapore, 2-7 August 2009.c2009 ACL and AFNLPQuantitative modeling of the neural ... coeffi-cients. The multiplicative model assumes the mean-ing of the composition is the element-wise prod-uct of the two vectors: vuCp ⋅⋅= Mitchell and Lapata (2008) fitted the parame-ters of the...

Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf

... ligands in the Mn-reconstituted enzyme and an additional water oxy-gen, which, together with the OD2 of Asp165, the NE2 of His79 and the NE2 of His169, formed anequatorial plane (Fig. 3B). The metal ... inhibi-tion of the binding of the superoxide substrate and the other to product inhibition by hydrogen peroxide.There are two mechanisms proposed for the reactioncycle of Mn ⁄ Fe-SOD: the 5-6-5 ... Mn-reconstitutedApeSOD, the metal ion was ﬁve-coordinate in trigonalbipyramidal geometry (Fig. 3A). Three of the ligands,OD2 of Asp165, NE2 of His79 and NE2 of His169,formed an equatorial plane. The other protein...

Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt

... this RNR and that of other class 1RNRs or, instead, is a result of the interaction of the R2F with the inactivated (oxidized) form of the R1E.We consider the ﬁrst option unlikely. Under these ... man-ganese in the transcriptional regulation of the nrdoperon [35] may not be considered in the light of the results obtained in the present study. Rather, the involvement of manganese in the in vivo ... to the catalytic site of the R1E subunit. Asreported in the Results, upon completion of substrateconversion, the radical is then rapidly passed backfrom the R1E subunit to the tyrosyl of the...

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