... [24,43,44].
Therefore, we performed genome-wide analysis to
examine the clustering patterns of the miRNAs in four
species of plant. The pairwise distance analysis results
of same-strand consecutive ... The Authors Journal compilation ª 2011 FEBS
Genome-wide analysis of clustering patterns and flanking
characteristics for plant microRNA genes
Meng Zhou...
... Sciences and Institute of Biotechnology, University of Granada, Spain;
2
Institute of
Protein Research, Russian Academy of Sciences, Pushchino, Moscow, Russia;
3
Faculty of Biology, Department of Biochemistry
and ... thermo-
dynamic parameters of the native, dimeric structure and its
thermal unfolding, and to describe some of the experimental
and mathematical bases...
... Structural and Functional Biology, and Centre of Neuroscience, University of Insubria, Busto Arsizio, Italy
2 Department of Biotechnology, University of Verona, Italy
3 Department of Clinical and Biological ... impairment of the antioxidant defense of the
cell, as in the case of DJ-1 or PTEN-induced putative
kinase 1 mutations. Mitochondrial and proteasome
dysfunction...
... The
observed spatial position of Ser224 and His438 does
not allow the formation of such a short, strong hydro-
gen bond. For example, the distance between Ne of
His438 and Oc of Ser224 is 4.8 A
˚
. ... site
concentration of wild-type BuChE was 0.16 ± 0.01 lm,
and that of the D70G mutant was 0.196 ± 0.02 lm.
Effects of ligands on steady-state kinetics
The effects of the...
... pool.
A further analysis of the influence of PQ reduction
on FI was performed after the addition of dPQ to the
thylakoid samples. Treatment of thylakoids with 1 lm
dPQ had no effect on E
A
for any phase. ... steps
of experimental FI traces obtained from thylakoid
membranes. Such analysis is needed for a a more thor-
ough use of FI in the study of PSII electron transport...
... ligands for plant
lectins in their native tissues [16–18].
The thermodynamic parameters DH° and DS° obtained
from the van’t Hoff analysis of the K
a
values for C uTCPP,
H
2
TPPS, CuTMPyP and ... 13%, yielding a total of 36%
b-sheet. Of the remainder, b-turns account for 21% of the
secondary structure of MCL, and unordered structures
comprise about 31%. The presence...
... ÔStains-
AllÕ analysis of Fig. 3 and the 1D i mmunoblotting of Fig. 4.
As the full-length Dp427 isoform of dystrophin does not
enter the second dimension of conventional 2D gels, the
expression level of ... the 2D pattern and
often result i n a str eaky spot pattern. Therefore, silver-
stained 2D patterns of muscle proteins probably overesti-
mate the presence of s olubl...
... with BMAL1 and the bin ding to the E-box.
Substitution of Arg65 for Ala abolished the interaction of
DEC1 with BMAL1 and the binding of DEC1 to the E-box.
However, substitution of His57 for Ala ... responsible for the sup-
pression, for the interaction with BMAL1 and for its
binding to CACG TG E-boxes. However, substitution of
His57 for Ala significantly reduced the...
... complex,
cleavage of the P
1
–P
1
¢ peptide bond, linkage of the active
site Ser of the protease to the carboxyl group of P
1
by an
ester bond and insertion of the N-terminal part of the RCL
as strand 4 ... in
terms of latency transition and functional behaviour upon
interaction with uPA and several side chains involved in
the retardation of latency transition, and hen...
... University of Groningen, the Netherlands;
3
Micro-Spectroscopy Centre, Laboratory of
Biochemistry, Wageningen University, the Netherlands;
4
Centre for Molecular and Biomolecular Informatics, ... techniques [13] and transfor-
mation of H. polymorpha were performed as described
previously [14]. Restriction enzymes and biochemicals were
from Roche (Almere, the Netherlands) and u...