Báo cáo khoa học: Crystal structures of open and closed forms of D-serine deaminase from Salmonella typhimurium – implications on substrate speciﬁcity and catalysis pptx

... al. Crystal structure of D-serine deaminase FEBS Journal 278 (2011) 287 9–2 891 ª 2011 The Authors Journal compilation ª 2011 FEBS 2891 Crystal structures of open and closed forms of D-serine deaminase ... D-serine deaminase from Salmonella typhimurium – implications on substrate speciﬁcity and catalysis Sakshibeedu Rajegowda Bharath1, Shveta Bisht1, Handanhal Subbarao Savithri2 and Mattur Ramabhadrashastry ... inabstraction of proton from the Ca atom of the substrate. Apart from thephysiological reaction, StDSD catalyses a, b elimination of D-Thr, D-Allothr and L-Ser to the corresponding a-keto acids and...

Báo cáo khoa học: Crystal structures of open and closed forms of cyclo⁄maltodextrin-binding protein pptx

... 21.7 – – – – 336 75.6 – – – – 337 61.7 – – – – 338 3.4Table 2. Angles C4–O4¢–C1¢ produced by two glucose residues in the CD molecules. –, not applicable.Numbers of the two glucose residues 1–2 ... P212121(TvuCMBP–G4) and C2(TvuCMBP–a-CD and TvuCMBP–b-CD) crystals weregrown at 20 °C under almost the same buffer conditions.Although the overall conformations of TvuCMBP–G4 and TvuCMBP–a-CD are ... TvuCMBP–a-CD and TvuCMBP–b-CD were determined to be in the closed form,whereas TvuCMBP–G4 adopted the open form.Results and DiscussionOverall structures of TvuCMBP–ligand complexesThe crystal structures...

Tài liệu Báo cáo khoa học: Crystal structures of the regulatory subunit of Thr-sensitive aspartate kinase fromThermus thermophilus pdf

... proceduresEnzyme production and crystallizationGene cloning and the production, puriﬁcation and crystalli-zation of TtAKb-Thr were performed as previouslydescribed [31]. TtAKb-free was crystallized by ... electron densities of the N-termi-nal (residues 1–3 in chain A, residues 1–4 in chains B and C, and residues 1–5 in chains E–G) and C-termi-nal (residues 15 8–1 61 in chains A–F, and residues15 9–1 61 ... models of the B chain (residues 1 5–9 3) from TtAKb-Thr and the F chain (residues 1 5–9 3) from TtAKb-free, and ACT2 shows Ca models of the A chain (residues 5–1 4 and 9 4–1 57) from TtAKb-Thr and the...

Tài liệu Báo cáo khoa học: Crystal structures of a bacterial 6-phosphogluconate dehydrogenase reveal aspects of speciﬁcity, mechanism and mode of inhibition by analogues of high-energy reaction intermediates pdf

... key features of PDH speciﬁcity and mode of inhibition of the enzyme.Results and DiscussionStructural analysis and model quality Crystal structures of three different complexes of LlPDH have ... compilation ª 2006 FEBS Crystal structures of a bacterial 6-phosphogluconatedehydrogenase reveal aspects of speciﬁcity, mechanism and mode of inhibition by analogues of high-energyreaction intermediatesRamasubramanian ... of the ligand.Fig. 2. Amino acid sequence and secondary structure of LlPDH. Arrows depict b strands, cylinders depict a helices and these are labelledb1–b10 and a1–a21. The elements of secondary...

Báo cáo khoa học: Crystal structures of the apo form of b-fructofuranosidase from Biﬁdobacterium longum and its complex with fructose pot

... network of loopsconnecting the strands is complicated; six loops con-nect b-strands from both b-sheets ( 1–1 ¢ 1 –2 2–2 ¢ 5 –3 5–6 ¢ 6 –6 ) and five loops connect b-strands within thesame b-sheet (2 –3 ¢ ... of reaction leading to retention of configura-tion on the anomeric carbon of the b-(2,1)-D-fructofuranose.Fig. 5. Dimer of b-fructofuranosidase from Bifidobacterium longum,the noncrystallographic ... crystal- lization conditions and formed different crystal forms depending on the pH value.Several crystal forms of b-fructofuranosidase wereobtained. Needle-shaped crystals were probablymonoclinic...

Báo cáo khoa học: Crystal structures of Nipah and Hendra virus fusion core proteins ppt

... the HR2 regions).Results and DiscussionStructure determinationThe HR1 and HR2 regions of the NiV and HeV Fproteins consist of residues 13 7–1 78 and residues45 3–4 85, respectively, and were predicted ... TheABCFig. 1. Construction and sequence alignment of the Nipah virus(NiV) and Hendra virus (HeV) fusion cores. (A) Prediction of theheptad repeat (HR) regions and the construction strategy for ... representation of oneHR1 helix and one HR2 helix [21]. Sequence compar-ison between NiV and SV5 fusion proteins shows thatﬁve out of nine changes (including one A to V) occurin the e and g positions...

Báo cáo khoa học: Crystal structures of the human SUMO-2 protein at 1.6 A and 1.2 A resolution ppt

... Photographs and electron density maps of the SUMO-2 crystals. Shown in (A) and (B) are two different crystal forms I and II obtaine d underslightly different conditions. The sizes of crystals are ... respectively,corresponding to about o ne-quarter and more than one-third of the contact surfaces. T he interactions include twohydrogen bonds between backbone atoms of Gly27(O )– Lys33*(N) and Val29(N)–Gln31*(O), ... geometry and crystal contacts wereanalysed using the programsPROCHECK and AREAIMOL of the CCP4 package [21].Results and DiscussionStructure determination and refinementAnalysis of the diffraction...

Báo cáo khoa học: Crystal structures of HIV protease V82A and L90M mutants reveal changes in the indinavir-binding site potx

... with theconformation of active-site residues. However, mutations of Arg8 and Glu21 are rarely observed in resistant isolates [2].One of the conformations of the side chain of Lys45 in PR forms ... chain of Met90¢ has two conformations, and one conformation has a short separation from the carbonyl oxy-gen of the catalytic Asp25¢.(B)ComparisonofMet90¢ in PRL90M and Leu90¢ in the wild-type ... independently of the location of themutation [ 8–1 4]. We have analyzed high-resolution crystal structures of the mature HIV-1 protease bearing eithersingle or double substitution mutations bound to substrate analogs...

Báo cáo khoa học: Crystal structures of bovine odorant-binding protein in complex with odorant molecules doc

... 1).ResultsBinding of AMA and odorants in solutionThe dissociation c onstants of the AMA–OBP c omplexes fo rthe bovine and porcine OBP isoforms were, respectively, 1 .0 and 1.5 lM and the corresponding ... Kdissfor AMA and theconcentration of AMA.Crystallization, data collection and reﬁnement of bovine OBPExcept for the bOBP–UND complex, bOBP crystals wereobtained by m icrodialysis of a 1 0 mgÆmL)1protein ... set of residues forming thecavity wall and do no t exhibit spe ciﬁc interactions.Depending on the ligand and on the monomer (A or B), asingle residue – Phe89 – presents alternate conformation...

Báo cáo khoa học: Crystal structures of Aedes aegypti kynurenine aminotransferase potx

... mgÆmL)1 of protein, 30% (w ⁄ v) PEG 1000, and 0.1 m of Tris ⁄ HCl at pH 8.5. Reﬁnement of preliminary crystalliza-tion conditions resulted in the growth of quality crystals ina solution containing ... volume of reservoir solu-tion at 500 lL and the drop volume at 5 lL, containing2.5 lL of protein sample and 2.5 lL of reservoir solution.AeKAT crystals were obtained in a solution containing10 ... activesites of the PMP and PLP forms. The map contoured at 2.0 sigmais calculated using data between 10.0 and 1.90 A˚ and 10.0 and 1.55 A˚resolution for the PMP and PLP forms of AeKAT, respect-ively....

Xem thêm