... greenalgae [3,4], including Chlamydomonas reinhardtii.Thisparticular alga is capable of evolving H2gas in the dark[5,6] or in the light, using H2O [7] or starch [8,9] as the source of reductant. ... are also present in the C. reinhardtii HydA2amino-acid sequence [33–35], including the well-conservedC-terminal part (C-domain) that binds the catalyticH-cluster. As seen in other algal [Fe]-hydrogenases, ... consists of eight exons and nineintrons (average intron size, 150 bp). The structuralarrangement of HydA2 is thus more complex than HydA1,which contains only seven introns [16]. Interestingly,...