Báo cáo Y học: The structure of the O-chain of the lipopolysaccharide of a prototypal diarrheagenic strain of Hafnia alvei that has characteristics of a new species under the genus Escherichia pot
Báo cáo Y học: The structure of the O-chain of the lipopolysaccharide of a prototypal diarrheagenic strain of Hafnia alvei that has characteristics of a new species under the genus Escherichia pot
... The structure of the O-chain of the lipopolysaccharide of a prototypal diarrheagenic strain of Hafnia alvei that has characteristics of a new species under the genus Escherichia Reine Eserstam1, ... the genus Escherichia. Keywords: lipopolysaccharide; Escherichia; Hafnia alvei; diarrhea; neuraminic acid. Hafnia alvei is a Gram negative bacterium and a member of the family Enterobacteriaceae. ... isolated in Bangladeshwas studied with sugar, and methylation analysis, NMRspectroscopy, mass spectrometry and partial acid hydrolysis. The strain was first designated as Hafnia alvei, but laterfound...
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Tài liệu Báo cáo Y học: Solution structure of the mEGF/TGFa44250 chimeric growth factor doc
... set of solutions reflects any uncertainty in the calibration as well as alternative fits to the set of distances. The main advantages of the procedure are simple: the details of the calibration are ... of theoretical cross relaxation rates. These valuesmay be replaced by scaled experimental values and the logarithm of the matrix may be taken to obtain distances that take account of spin diffusion ... Typically, however,such calculations generate some nonphysical negative crossrelaxation rates as a consequence of the failure of the approximation of a rigid molecule and because of inaccuracies...
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Báo cáo Y học: Solution structure of the Alzheimer amyloid b-peptide (1–42) in an apolar microenvironment Similarity with a virus fusion domain potx
... stronglyhydrophobic side chains; this feature has been aptlydescribed by Rajan et al. as a Teflon coating that cansurround a helix [16] in the case of a mixture of water andhexafluoroacetone hydrate, ... exploratory search because it can dissolveAb-(1–42) better than all other media and, at the same time, it has a helix-promoting ability very similar to that of trifluoroethanol [15,16].MATERIALS ... was adopted for the elution of the peptide. The fraction containing the pure peptide was lyophilized twiceand the purity assessed by a MALDI-TOF analysis using a Hewlett Packard G202 5A LD-TOF...
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Báo cáo Y học: Solution structure of the HIV gp120 C5 Domain pptx
... shownby Fig. 5A, the amino terminal end of the C5 domainwould be attached to remaining domains of gp120 in the native structure. The carboxy terminal end of the C5domain would be attached to the ... helix. The tertiary structure of HIV gp120 C5 was determinedby an iterative method of torsion angle molecular dynam-ics followed by simulated annealing [18]. A summary of theNOEsusedinthecalculationispresentedinFig.3.From ... inFig. 4A and the structural statistics of the ensemble aresummarized in Table 1. The overall backbone RMSD of the ensemble to the mean is 0.53 A ˚and the overall heavyatom RMSD (i.e. nonhydrogen)...
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Báo cáo Y học: Crystal structure of the catalytic domain of a human thioredoxin-like protein pdf
... hTRXL-N may accountfor the formation of a monomer, instead of a dimer in the case of TRX. Furthermore, the loss of intermoleculardisulfide-bonds and the disbandment of the hydrophobicpatch may also ... model, then refined smoothly inalternating steps of automatic adjustment with CNS andmanual adjustment with the program O [34]. The finalmodel has a final R-factor of 0.222 with a free R-factor of 0.253. ... protein with289 amino acids and a calculated molecular mass of 32 kDa. A search of the nonredundant protein sequencedatabase was performed using the BLASTprogram. Besidessharing the same sequence...
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Báo cáo Y học: Chemical structure and immunoreactivity of the lipopolysaccharide of the deep rough mutant I-69 Rd–/b+ of Haemophilus influenzae docx
... 1. HPAEC chromatogram of deacylated LPS from H. influenzaeI-69. Shown is the analytical separatio n of the crude mixture (A) and the a nalytical chromatography of the isolated species (B a nd ... beseparated at t hat time and were not yet chemicallysynthesized, the s pecificity of this m Ab has not yet beenelucidated. Here, we report on: (a) t he successful separation of the deacylated carboh ... surface antigen leadingto the induction of protective a ntibodies; and (d) the understanding of the biosynthesis of LPS may allow the distinct blockage of e ssential steps as a new strategyfor...
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Báo cáo Y học: Solution structure of a hydrophobic analogue of the winter flounder antifreeze protein doc
... chemical shift changes wereTable 1. Sequence alignment of TTTT and VVVV2KE.1 2 13 24 35TTTT D TASDAAAAAAL TAANAKAAAEL TAANAAAAAAA TARVVVV2KE D VASDAKAAAEL VAANAKAAAEL VAANAKAAAEA VARCONH21260 ... carboxyl group of Asp1 and the backbone amide of Ser4. The C-terminal cap structure, however, makes use of the Arg37 side chain to form a hydrogen bond to the backbone carbonyl oxygen of Ala33 ... eight of the 20 NMR conformers. Infour of these, the carbonyl oxygen of Ala14 was hydrogenbonded to the amide proton of Ala17 instead. A straighthelix around Lys18 was obtained in test calculations,...
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Báo cáo Y học: NMR structure of the HIV-1 regulatory protein Vpr in H2O/trifluoroethanol Comparison with the Vpr N-terminal (1–51) and C-terminal (52–96) domains pot
... Tyr50 a 2Gly563.5H Tyr50 a 2Gly562.6H Tyr50 a Ala593.5H Tyr50 a Ala592.6H Tyr50 b Ala593.5H Tyr50 b Ala59b1Tyr50 a 1Gly56b2Tyr50 a 1Gly56b1Tyr50 a 2Gly56b2Tyr50 a 2Gly56b1Tyr47 ... a semipreparative Vydac C18column using a linear gradient of acetonitrile. An experimental mass of 11 431.92 Da wasobtained by electrospray mass spectroscopy and a mass of 11 433.02 Da was calculated ... with the lowest values for total energy and NOE restraintviolations were analysed with respect to the rmsd values of the backbone. The structural stability has been examined under minimization and...
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Báo cáo y học: "Technical Considerations in Decompressive Craniectomy in the Treatment of Traumatic Brain Injury"
... problem.39 After craniotomy, removal of the in-tracranial lesion, and duraplasty, the bone flap was replaced and one side of the flap was attached to the cranium by plates. The plates act as a hinge ... fascia from the temporal muscle to the zygomatic arch, and then cut the fascia from the base backwards along th e zygoma but left the fascia base 1-2 cm long for the blood supply. Finally, ... adhesions, the authors placed a dural substi-tute between the dural anasynthesis flap and galea aponeurotica after augmentative duraplasty with temporal muscle. They performed this method in 23 patients...
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Báo cáo y học: "Mechanical complications and reconstruction strategies at the site of hip spacer implantation"
... 1). Alterna-tively, a partial (Figure 2) or normal cementation of the spacer into the femoral canal provides the advan-tage of rotational and axial stability [3]. A normal ce-mentation has the ... mantle and a spacer head is an elegant method that treats both the fracture and the infection (Figure 8). At the time of prosthesis reimplantation, the spacer head can be easily removed and the ... disadvantage in comparison with the partial cementation that all cement debris have to be removed from the femoral canal during the later prosthesis reimplantation, and that during removal of...
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