Báo cáo Y học: Identification of a critical lysine residue at the active site in glyceraldehyde-3-phosphate dehydrogenase of Ehrlich ascites carcinoma cell ppt
Báo cáo Y học: Identification of a critical lysine residue at the active site in glyceraldehyde-3-phosphate dehydrogenase of Ehrlich ascites carcinoma cell ppt
... Identification of a critical lysine residue at the active site in glyceraldehyde-3-phosphate dehydrogenase of Ehrlich ascites carcinoma cell Comparison with the rabbit muscle enzymeSwapna Ghosh1, ... other hand, confirm that an essential lysine residue isinvolved in the catalytic activity of the EAC cell enzyme.This difference in the nature of the active site of EAC cell Gra3P DH that may ... Biochemistry,University College of Science, University of Calcutta, India The involvement of the lysine residue present at the active site of Ehrlich ascites carcinoma (EAC) cell glyceralde-hyde-3-phosphate dehydrogenase...
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Tài liệu Báo cáo Y học: Identification and characterization of a mammalian 14-kDa phosphohistidine phosphatase pdf
... supernatant was used for purification of the recombinant protein.Purification of the recombinant phosphohistidinephosphatase A 9-mL sample of the supernatant of the bacterial lysatewas chromatographed ... Protein kinases and phosphatases that acton histidine, lysine, or arginine residues in eukaryotic proteins: a possible regulator of the mitogen-activated protein kinase cascade.Pharmacol. Ther. ... phosphohistidine-containing pro-teins to be considered as possible natural substrates of the phosphohistidine phosphatase is that of enzymes that areintermediary phosphorylated on a histidine of the active site. ...
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Tài liệu Báo cáo Y học: Identification of a set of genes involved in the biosynthesis of the aminonucleoside moiety of antibiotic A201A from Streptomyces capreolus pdf
... suggest that certain enzymes, andtherefore the corresponding genes of the A2 0 1A biosyn-thetic pathway, may be related to their counterparts of the puromycin and hygromycin A biosynthetic pathways,respectively. The ... Further identification and quantification of puromycin were achieved by a Pac enzymatic assay[21].Preparation of 3¢-amino-3¢-deoxyadenosine3¢-amino-3¢-deoxyadenosine was obtained from Helmin-thosporium ... orientation(Figs2and 3A) .Ofthese,five(ataP3, ataP5, ataP4, ataP10and ataP7) are highly similar to known or putative genesthat are known or proposed to be implicated in the biosynthesis of the aminonucleoside...
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Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx
... shown that GatY aggregates at 42 °Cinmutantcells containing a deletion for DnaK [35]. Taken together,these observations indicate that maintenance of the correctfolding state of GatY in E. coli ... itssteady-state level increases further during the late stationaryphase. As a result of this accumulation, the relative amount of UP12 in stationary cells is about 10 times higher than thatobserved ... stationary phase the rate of synthesis of heat-shock proteins increases considerably,but only transiently [2,18]. Similarly, we observed that the steady-state amount of GroEL remains constant...
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Báo cáo Y học: Identification and characterization of a new gene from Variovorax paradoxus Iso1 encoding N -acyl-D-amino acid amidohydrolase responsible for D-amino acid production pdf
... specificity of the N-D-AAase protein, the activity of the enzyme against N-acyl-D-orL-amino acidsand otherD-amino acid derivates was determined (Table 3). The substrates analysed were a range of ... pH 6 and pH 8, 80% activity remained. The N-D-AAase had higher hydrolysing activity against N-ace-tyl-D-amino acid derivates containingD-methionine,D-leucine andD-alanine and against ... [12,13].N-acyl-D-amidohydrolase (EC.3.5.1.81, N-D-AAase) is anenzyme capable of catalysing the hydrolysis of N-acyl-D-amino acids to yield the correspondingD-amino acid and the organic acid. They have been...
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Báo cáo Y học: Identification of residues critical for activity of the wound-induced leucine aminopeptidase (LAP-A) of tomato pptx
... C a of Asp a nd its carboxylate oxygen,C a of Arg and its side-chain amines or guanidinium, and C a of Lys and its side-chain amine was determined bymeasuring distances in the blLAP. These average ... bovine and prokaryotic LAPs and a limited mutationalanalysis of the E. coli PepA have suggested Lys262 andArg336 have a role in catalysis [22,23]. The residues implicated in zinc binding and ... function of the LAP enzymes. The compelling sequence identities in LAP enzymessuggested that the tomato LAP -A may u se a c atalyticmechanism similar to that employed by the animal andbacterial LAPs...
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Báo cáo Y học: Identification and characterization of a novel activated RhoB binding protein containing a PDZ domain whose expression is specifically modulated in thyroid cells by cAMP pot
... was secondary to the activation of the adenylyl cyclase/cAMP pathway, the same experiments were performed using 10)5Mforskolin,an adenylate cyclase activator. A pattern similar to the thyrotropin ... cytoplasmicstaining with a clear perinuclear accumulation (Fig. 4B).Moreover p76RBEis, at least partially, located in the cell plasma membrane as indicated by arrows (Fig. 4B). The same pattern was ... (methymazole/propylthiouracil) treated dogthyroid cDNA library [23]. This screening was aimed at isolating new proteins whose expression was regulated by the thyrotropin-dependent pathway. Analysis of the aminoacid...
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Tài liệu Báo cáo Y học: Identification of syntaxin-1A sites of phosphorylation by casein kinase I and casein kinase II ppt
... DISCUSSIONPhosphorylation a nd dephosphorylation of protein su b-strates by k inases and phosphatases are enzymatic activitiesthat play prominent roles in many biological processesincluding synaptic vesicle ... residue( s) a re locatedwithin t he N-terminal moiety of syntaxin- 1A (Fig. 2). Ourdata support previous reports indicating that CKII phos-phorylates the N-terminal 75 amino acids of syntaxin- 1A while ... indicated. The main peptidesrecovered after trypsin digestion of syntaxin- 1A phosphorylated by CKIa and CKII are indicated by the bars. Where two masses are shown theseare the doubly then singly...
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Báo cáo Y học: Identification of the 19S regulatory particle subunits from the rice 26S proteasome potx
... Biological Research/Graduate School of Integrated Science, Japan, personal communication) iden-tified N-acetylation of many of the yeast 26S proteasomesubunits (a1 , a2 , a3 , a4 , a5 , a6 , a7 , a3 , a5 , ... 9Sregulatory particles (RP; 700 kDa). The 20S proteasomehas a cylindrical structure, consisting of a and b ringsstacked in the order of abba, and each ring contains sevenstructurally related a and ... of them in both cytoplasm a nd nucleus of eukaryoteis ubiquitin-proteasome pathway that involves the covalentattachment of polyubiquitins to substrate targeted fordegradation. The ubiquitinated...
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Báo cáo Y học: Identification of residues in the PXR ligand binding domain critical for species specific and constitutive activation docx
... amino-acid analyzer combines the OPA andFMOC as derivatization reagents for the complete detection of all residues. BSA was used as a standard protein tocalculate the amount of protein in the ... create a more pronounced change than the spatially and electrostatically moderate change of a histidineto a glutamine and thereby give additional information intoits potential role in ligand ... shows a high basal activity,it can be further activated by the potent activator rifampicin.If the mutation triggers specific conformational changesthat facilitate receptor activation, the binding...
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