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Báo cáo Y học: Bromoperoxidase activity of vanadate-substituted acid phosphatases from Shigella flexneri and Salmonella enterica ser typhimurium doc

Báo cáo Y học: Bromoperoxidase activity of vanadate-substituted acid phosphatases from Shigella flexneri and Salmonella enterica ser. typhimurium doc

Báo cáo Y học: Bromoperoxidase activity of vanadate-substituted acid phosphatases from Shigella flexneri and Salmonella enterica ser. typhimurium doc

... Bromoperoxidase activity of vanadate-substituted acid phosphatases from Shigella flexneri and Salmonella enterica ser. typhimurium Naoko Tanaka1, Vale´rie Dumay1, Qianning ... are thenonspecific acid phosphatase from Shigella flexneri (PhoN-Sf) and the enzyme from Salmonella enterica ser. typhimurium (PhoN-Se) [13,14]. On the basis of sequencesimilarity, it has been proposed ... assay kit (Bio-Rad) with BSA as the standard.Enzymatic assay of phosphatase activity The phosphatase activity was measured by hydrolysis of 10 mMp-nitrophenyl phosphate as a substrate in 100...
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Báo cáo Y học: Amidolytic activity of prostatic acid phosphatase on human semenogelins and semenogelin-derived synthetic substrates docx

Báo cáo Y học: Amidolytic activity of prostatic acid phosphatase on human semenogelins and semenogelin-derived synthetic substrates docx

... conditions.Conversely, acid phosphatase activity was measured inthe presence of NaF and of those of inhibitors that alteramidolytic activity. Incubation with inhibitors was per-fromed at pH 9 and at pH ... amidolytic activity was obtained by showing thatcommercial PAP hydrolysed the Abz-ISYQSSSTEEQ-EDDnp substrate at neutral pH, as does the puri®edenzyme. The ratio of the amidolytic activity of ... purit y by SDS/PAGE and C4 reversed-phase chromatography using a gradien t of 0±60% acetonitrile in 0.075% CF3COOH.Fig. 2. pH activity pro®le of PAP activity (full line) and PAP amidolyticactivity...
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Báo cáo y học:

Báo cáo y học: "Anticancer Activity of the PR Domain of Tumor Suppressor RIZ1"

... Chen1, Binbing Ling1, Ramaswami Sammynaiken3 and Jian Yang1,  1. Drug Design and Discovery Research Group, College of Pharmacy and Nutrition, University of Saskatchewan, 110 Science Place, ... IIB of ovarian cancer has only one elderly patient (80 years and older), the RIZ1 mRNA expression increase may not be necessarily representative for the disease stage. Anticancer activity of ... regions between RIZ1 and ephrin receptor (EphR) during sequence analysis (Fig. 2). The sequence identity and homology were 34.2% and 68.4%, 37.0% and 81.5%, and 22.9% and 53.1% in the three...
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Báo cáo Y học: Transcriptional activity of interferon regulatory factor (IRF)-3 depends on multiple protein–protein interactions pdf

Báo cáo Y học: Transcriptional activity of interferon regulatory factor (IRF)-3 depends on multiple protein–protein interactions pdf

... intrinsictranscriptional activity. Fig. 2. The transcriptional activity of IRF-3 depends on synergy betweentwo sets of residues and is entirely dependent on mammalian CBP. (A)Transcriptional activity in S2 cells of ... transcriptional activity of IRF-3 is dependent on simultaneous contact withboth the N- and C-termini of CBP, and on the physicalintegrity of CBP.We also tested the ability of IRF-3E7 and human p300 ... thatmutation of either set of Ser/ Thr residues alone isaccompanied by a virus-dependent increase in activity, and that mutation of both sets is not, strongly suggest thatresidues within both sets of Ser/ Thr...
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Báo cáo y học:

Báo cáo y học: "ntravenous transplantation of allogeneic bone marrow mesenchymal stem cells and its directional migration to the necrotic femoral head"

... Department of Orthopedics, Affiliated Hospital of Hebei University, Baoding 071000, China. 3. Laboratory of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Academy of Military ... arthroplasty [30,31]: Currently, the efficacy of arthroplasty has been significantly im-proved. However, nowadays, a lot of younger adults develop ischemic femoral head necrosis, and lifetime of ... refreshed, and then the medium changed every other day. Cell passaging was per-formed when cell confluence reached 85%. The purity and immunophenotype of MSCs and their potentials of osteogenesis and...
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Báo cáo y học:

Báo cáo y học: "The Syndrome of Frontonasal Dysplasia, Callosal Agenesis, Basal Encephalocele, and Eye Anomalies – Phenotypic and Aetiologica"

... different pattern of cerebral and facial manifestations ranging from normality to typical HPE phenotype. Penetrance is relatively low. Patient 1 of the present study was previously reported as ... comprises of the syndrome of midline facial defects, callosal agenesis, basal encephaloceles, and eye anomalies. It has been described under different eponyms, most commonly the morning glory syndrome. ... acrofrontofacionasal dysostosis 2 (MIM 201181), frontofacionasal dysplasia (MIM 229400), oculoauriculofrontonasal syndrome (MIM 601452), among other related conditions [1]. One of the syndromes...
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Báo cáo y học:

Báo cáo y học: "Different effect of exercise on left ventricular diastolic time and interventricular dyssynchrony in heart failure patients with and without left bundle branch block"

... exercise LV dyssynchrony may play a more important role in the pathophysiology of left ven-tricular remodelling than baseline LV dyssynchrony (23). Probably for that reason additional hemodynamic ... mechanical dyssynchrony. These findings may further explain why patients with normal QRS duration may benefit from cardiac re-synchronization whether or not they have mechanical dyssynchrony under ... highly dynamic nature of mechanical ventricular dyssynchrony in heart failure patients with normal QRS duration. By characterizing mechanical interventricular dyssynchrony, which was commonly...
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Báo cáo Y học: IgE reactivity of tandem repeats derived from cockroach allergen, Bla g 1 docx

Báo cáo Y học: IgE reactivity of tandem repeats derived from cockroach allergen, Bla g 1 docx

... structural studies and a candidate forclinical use in diagnosis of cockroach allergy and develop-ment of new forms of immunotherapy.Keywords: allergen; hypersensitivity; IgE antibody; asthma;cockroach.Sensitization ... inoculate 500 mL of medium and grown for 7 days.Analysis of transformants for integration of Bla g 1 DNAinP. pastorisgenome.Yeast genomic DNA was isolated from pPICZaB clonesselected by growth ... yeastgenome.Purification and sequencing of rBla g 1Expressed rBla g 1 was purified from culture media byaffinity chromatography over a 10A6 monoclonal antibodycolumn as described previously...
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Báo cáo Y học: Molecular cloning of columbamine O-methyltransferase from cultured Coptis japonica cells pot

Báo cáo Y học: Molecular cloning of columbamine O-methyltransferase from cultured Coptis japonica cells pot

... analyzedsubstrate affinity of CoOMT for tetrahydrocolumbamineusing this 80% pure tetrahydrocolumbamine.To quantify the enzymatic activity of CoOMT, thetransfer of the3H-labeled methyl group ... synthesized and purified from berberineby two-step reactions according to the method of Cava and Reed [20,21].Construction and sequencing of cDNA library of C. japonicaPoly(A)+RNA was isolated, and ... ahigh degree of similarity (approximately 40% identity) toCoptis 6OMT, Coptis 4¢OMT, IOMT of alfalfa [25], and 6a-hydroxymaackiain 3-O-methyltransferase of pea [26].The deduced polypeptide showed...
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Báo cáo Y học: Coordinated action of protein tyrosine phosphatases in insulin signal transduction potx

Báo cáo Y học: Coordinated action of protein tyrosine phosphatases in insulin signal transduction potx

... SH2domain of SHP-2 and phosphotyrosine 1146 of the activatedinsulin receptor [66]. Insulin also induces the formation of acomplex of IRS-1 and SHP-2, requiring the tyrosines 1172 and 1222 of IRS-1 ... both necessary and sufficient for its targeting to theER [81]. Cleavage of this segment appears to release theenzyme from the ER and increase its specific activity [83]. Byin situ hybridization, ... of PTP-1B protein into Xenopus oo cytesdecreases t yrosine phosphorylation of proteins correspond-ing to the molecular m ass of the IR. Correspondingly,insulin-induced S6 kinase activity and...
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