... weight))1at stage 1, which was lessthan half of that in ovary at stage 1, and remained at a similar value to stage 4. Testes at stage 2 were notanalyzed because of a lack of samples. We calculated the ... stage 1, andeggs. In all of the samples analyzed, a large proteinpeak was observed at an elution position of 72 mL(peaks a, b, c, and d), where the estimated molecularmass was about 600 kDa. ... measured by the Bradford method [51] using a Bio-Rad protein assay (Bio-Rad, Hercules, CA, USA)with bovine c-globulin as a standard. Thyroglobulin(669 kDa), catalase (232 kDa), BSA (67 kDa) and...