... collagenases cur-rently include the ‘classical’ collagenases, MMP-1,MMP-8 and MMP-13 [2–4] and also the gelatinolyticenzyme, MMP-2 [5–7], and MMP-14 (MT1-MMP) [8],a member of the membrane-type subclass ... MMP-9has been reported previously [21]. MMP-9-mediateddigestion (both collagenolytic and telopeptidase) was -+ -+ -+ MAB911proMMP-9+ APMAMMP-1 MMP-13β11β12α1α2bufferMMP - 1TTdenat -+ +++++++proMMP-9APMAEDTAserine/+++ - ++++ - ... the MMP-1-generatedthree-quarter piece (Fig. 4, type III, compare MMP-1with + proMMP-9, + APMA) [26]. Digestion withproMMP-9 and APMA was abolished by EDTA,1,10-phenanthroline and TIMP-2, but...