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Characterization of mercury bioremediation by transgenic bacteria expressing metallothionein and polyphosphate kinase pdf

Characterization of mercury bioremediation by transgenic bacteria expressing metallothionein and polyphosphate kinase pdf

Characterization of mercury bioremediation by transgenic bacteria expressing metallothionein and polyphosphate kinase pdf

... Open Access Characterization of mercury bioremediation by transgenic bacteria expressing metallothionein and polyphosphate kinase Oscar N Ruiz*, Derry Alvarez, Gloriene Gonzalez-Ruiz and Cesar ... bioremediation efficiency of transgenic bacteria expressing metallothio-nien and polyphosphate kinase to understand which of these genes is best suited for mercury bioremediation. Finally, we characterized the bioremediation ... and mercury accumulation. More stu-dies are needed to further understand the processes of mercury absorption, accumulation, and resistance in transgenic bacteria expressing metallothionein and...
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Tài liệu Báo cáo khoa học: Characterization of electrogenic bromosulfophthalein transport in carnation petal microsomes and its inhibition by antibodies against bilitranslocase docx

Tài liệu Báo cáo khoa học: Characterization of electrogenic bromosulfophthalein transport in carnation petal microsomes and its inhibition by antibodies against bilitranslocase docx

... analysed by means of sigmaplot 2001 (SPSSScience Software Gmbh, Erkrath, Germany). Data for the characterization of the kinetics of electrogenic BSP uptakefitted the Michaelis–Menten equation and ... Sigma-Aldrich and Carlo Erba (Milan, Italy), and were of the highest available grade.AcknowledgementsThanks are due to Prof G.L. Sottocasa and Dr Anto-nella Bandiera (University of Trieste) ... bilirubin and nicotinic acid reduce the rate of BSP uptake inhi-bition by antibody A, an effect depending on the for-mation of a complex between the carrier and theligands [39]. The occurrence of...
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Báo cáo khoa học: Characterization of HbpR binding by site-directed mutagenesis of its DNA-binding site and by deletion of the effector domain pot

Báo cáo khoa học: Characterization of HbpR binding by site-directed mutagenesis of its DNA-binding site and by deletion of the effector domain pot

... Tropel and J. R. van der Meer1766 FEBS Journal 272 (2005) 1756–1766 ª 2005 FEBS Characterization of HbpR binding by site-directedmutagenesis of its DNA-binding site and by deletion of the effector ... Switzerland2 Department of Fundamental Microbiology, University of Lausanne, SwitzerlandThe metabolism of 2-hydroxybiphenyl (2-HBP) inPseudomonas azelaica HBP1 is initiated by enzymesencoded by ... Identification and physical characterization of the HbpR binding sites of the hbpC and hbpD promoters. J Bacteriol 184, 2914–2924.20 Jaspers MC, Sturme M & van der Meer JR (2001)Unusual location of...
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Báo cáo Y học: Characterization of heparin binding by a peptide from amyloid P component using capillary electrophoresis, surface plasmon resonance and isothermal titration calorimetry ppt

Báo cáo Y học: Characterization of heparin binding by a peptide from amyloid P component using capillary electrophoresis, surface plasmon resonance and isothermal titration calorimetry ppt

... Linhardt1 and Niels H. H. Heegaard21Division of Medicinal and Natural Products Chemistry, Department of Chemistry and Department of Chemical Engineering,University of Iowa, USA;2Department of ... circles) as a function of thetotal peptide concentration. A stock solution consisting of 125 lMscrambled peptide and 62 lM of each of the AP-1 and the mAP-1peptides and 41 lM of the marker peptide ... v3.2 software.Buffers were filtered and deoxygenated and all experimentswere carried out at 25 °C.Immobilization of regular and scrambled AP peptideson carboxymethylated chipsRegular and scrambled...
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Tài liệu Báo cáo khoa học: Molecular characterization of Arabidopsis thaliana PUF proteins – binding specificity and target candidates doc

Tài liệu Báo cáo khoa học: Molecular characterization of Arabidopsis thaliana PUF proteins – binding specificity and target candidates doc

... that of other well-characterized members of PUF family. These proteinsmust bind to transcripts in the plant and regulate theirtranslation and ⁄ or turnover.A large number of APUM target candidates: ... wild-type and CLV-1 were synthesized and annealed. Each annealedpair of oligonucleotides possess a 32 bp sequence of a3¢ UTR region of transcripts containing the APBE, a 5¢AvrII or XbaI site and ... in the 3¢ UTR. In recent years, and as a result of great efforts aiming to understand the mechanism of RNA control in animals, the function of a diversenumber of RNA-binding proteins has been...
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Tài liệu Báo cáo khoa học: Modulation of sterol homeostasis by the Cdc42p effectors Cla4p and Ste20p in the yeast Saccharomyces cerevisiae pptx

Tài liệu Báo cáo khoa học: Modulation of sterol homeostasis by the Cdc42p effectors Cla4p and Ste20p in the yeast Saccharomyces cerevisiae pptx

... interactedTable 2. Sterol analysis of cells overexpressing STE20 and CLA4.Data are mean values with standard deviation from at least twoindependent experiments.lg of sterol per mg of proteinpRS425 pRS425-STE20 ... Deletion of CLA4 results in higher amounts of SE. (A) Quan-tification of SE in cell polarity mutants. Cells of the indicated strainswere grown to stationary phase and then lipids were extracted and separated ... 1353–1356.35 Yu C, Kennedy NJ, Chang CC & Rothblatt JA (1996)Molecular cloning and characterization of two isoforms of Saccharomyces cerevisiae acyl-CoA:sterol acyltrans-ferase. J Biol Chem 271,...
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Tài liệu Báo cáo khoa học: Biochemical characterization of Bacillus subtilis type II isopentenyl diphosphate isomerase, and phylogenetic distribution of isoprenoid biosynthesis pathways doc

Tài liệu Báo cáo khoa học: Biochemical characterization of Bacillus subtilis type II isopentenyl diphosphate isomerase, and phylogenetic distribution of isoprenoid biosynthesis pathways doc

... terminated by theaddition of EDTA to a final concentration of 26 mM.Afterthe addition of D2O to a final concentration of 10% (v/v),the samples were analysed by NMR spectroscopy.Assay of lactate ... accompanied by the appearance of three new13C-coupled signals at143.4, 111.3 and 21.4 p.p.m. assigned as the carbon atoms3, 4, and 5 of IPP, respectively (cf. Table 1 and Fig. 6).Within the limits of ... this context that both types of isomerases are foundin the Actinobacteria group. The anomalous positions forsome eubacterial species (e.g. Cyanobacteria and Actino- bacteria) observed here (cf....
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ASSESSMENT OF DIAGNOSIS OF PULMONARY TUBERCULOSIS BY SPUTUM MICROSCOPY IN A DISTRICT TUBERCULOSIS PROGRAMME pdf

ASSESSMENT OF DIAGNOSIS OF PULMONARY TUBERCULOSIS BY SPUTUM MICROSCOPY IN A DISTRICT TUBERCULOSIS PROGRAMME pdf

... trained personnel and their replacement by untrained ones, inadequate supply of stains and other requirements, and quality of supplies particularly stains and slides. Some of these aspects ... to staff of general health services and periodic supervision followed by corrective actions, to ensure proper standards of work. In actual practice, the type of training imparted and the thoroughness ... was satisfactory and these might not have influenced results adversely. For a number of reasons, one of which is the influence of the training programme of NTI, the findings of this study should...
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A Synopsis of Social Marketing by Lynn MacFadyen, Martine Stead and Gerard Hastings (1999) pot

A Synopsis of Social Marketing by Lynn MacFadyen, Martine Stead and Gerard Hastings (1999) pot

... channels and designing appeals, implementing long term positioning strategies, and organisational and management issues (Bloom and Novelli, 1981). Lefebvre and Flora (1988) and Hastings and ... starts and finishes with research, and research is conducted throughout to inform the development of the strategy. A situational analysis of the internal and external environment and of the ... Dick Manoff, and Bill Novelli. Early examples of social marketing emerged during the 1960s as part of international development efforts in third world and developing countries (Manoff 1985,...
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Báo cáo khoa học: Biochemical characterization of USP7 reveals post-translational modification sites and structural requirements for substrate processing and subcellular localization pptx

Báo cáo khoa học: Biochemical characterization of USP7 reveals post-translational modification sites and structural requirements for substrate processing and subcellular localization pptx

... variantsEvaluation of the hydrolysis of Ub-AMC and Ub-K-TAMRA by USP7-FL and its domain deletionFig. 4. Enzymatic characterization of USP7. (A) Progress curves for the USP7-catalyzed hydrolysis of Ub-AMC ... proteolysis of USP7 variants in the presence and absence of ubiquitin. SDS ⁄ PAGE (4–20% gradient gels) showing thelimited proteolysis of native USP7-FL and variants thereof by trypsinover time with and ... specialized in the process-ing of ubiquitin and ubiquitin-like proteins. They areresponsible for the disassembly of ubiquitin chains, and for the cleavage of mono- and oligomers of this mole-cule, either...
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