Báo cáo Y học: Purification, characterization and subunits identification of the diol dehydratase of Lactobacillus collinoides pot
Báo cáo Y học: Purification, characterization and subunits identification of the diol dehydratase of Lactobacillus collinoides pot
... expression of the L. collinoides diol dehydratase The three genes pduCDE encoding the three subunits of the diol dehydratase were cloned in the pQE30 expression vectorsystem. The synthesis of the three ... study, wereport the purification, enzymatic characterization and analysis of the composition of the diol dehydratase of L. collinoides. MATERIALS AND METHODSBacteria and culture conditions The ... notbelong to the dehydratase complex. Characterization of the diol dehydratase The molecular mass of the native dehydratase was estimatedby the Sephacryl S300H gel filtration using five standards of known...
- 7
- 392
- 0
Báo cáo Y học: Two 1 : 1 binding modes for distamycin in the minor groove of d(GGCCAATTGG) docx
... hydrogen bonds with the thymine or adenine bases. The central N5(D) atom of the drug is hydrogen-bonded to the two O2 atoms of the thymine bases of the central AT steps. Nitrogen atomsN3(D) and ... drug hydrogen-bonds with the bases of only one DNA strand and stacks with the sugarrings.We have previously reported the structure determin-ation at 1.9-A˚resolution of the complex of the ... nucleotides of strand 1are labeled G1–G10 in the 5¢fi3¢ direction and G11–G20on strand 2, and the drug is labeled D. After determination of the weighting factor and positional adjustment of parts of the...
- 10
- 411
- 0
Tài liệu Báo cáo Y học: Purification, characterization, cloning, and expression of the chicken liver ecto-ATP-diphosphohydrolase pot
... ATPaseactivity at either pH 7.4 or pH 6.4 (Fig. 3). At pH 7.4,5mMazide inhibited ADP hydrolysis by 70% whereasATP hydrolysis was inhibited by only 10%. Inhibition of ADP and ATP hydrolysis by azide ... apical membranes of the oxyntic-peptic cells [37]. The distribution of the ecto-ATPDase on these epithelial cells is distinctly different fromtheotherATPDaseintheE-ATPasefamily,theCD39s[13,17,19].Molecular ... 0.2 and 0.64 depending on the pH and the presence of NP-40; (d)Fig. 3. Inhibition of ATP and ADP hydrolysis activities of purifiedchicken liver ecto-ATPDase by azide. ATP and ADP hydrolysis...
- 10
- 694
- 0
Tài liệu Báo cáo Y học: Purification, characterization, immunolocalization and structural analysis of the abundant cytoplasmic b-amylase from Calystegia sepium (hedge bindweed) rhizomes ppt
... method.Inhibition of the enzyme activity by glucose, maltose and cyclohexaamyloseFor the study of the enzyme inhibition by glucose, maltose and cyclohexaamylose b-amylase activity was measuredusing the iodine ... soybean b-amylase (Cys82, Cys97, Cys208 and Cys343).On the analogy of the soybean b-amylase, the activesite of the C. sepium b-amylase most probably consists of a cleft located between the barrel ... and the b-amylase fromsweet potato and soybean, respectively.Northern blot analysisNorthern blot analysis was performed to determine the totallength of the mRNA encoding the b-amylase. Hybridizationof...
- 11
- 611
- 0
Báo cáo Y học: Purification and biochemical characterization of some of the properties of recombinant human kynureninase pptx
... (PLP)-dependent enzyme which catalyses the b,c-hydrolytic cleavage of the amino acidsL-kynurenine (1 )and L-3-hydroxykynurenine (2)togiveL-alanine (3) and eitheranthranilic acid (4) or 3-hydroxyanthranilic ... regulation of enzyme activity in vivo and consequent channeling of substrate 3-hydroxykynurenine down the tryptophan–kynurenine metabolic pathway. The purification of recombinant human kynureninaseto ... pathophysiology of human diseaseshas always been hampered by the availability of humantissue, aesthetics, and ethical considerations. The principleaim of this study was to express a clone of humankynureninase...
- 6
- 406
- 1
Báo cáo Y học: Purification and characterization of novel kininogens from spotted wolffish and Atlantic cod pdf
... (saponification) of the N-glycans yielded glycans devoid of additional acetylgroups, an indication that these were O-acetyl substituents[15]. The MALDI-TOF spectrum of the de-O-acetylatedglycans reveals the ... belonging to class 3 of the cystatinsuperfamily. They are single-chain proteins composed of anN-terminal heavy chain, the bradykinin moiety and aC-terminal light chain. The heavy chain and light chain ... Kalkkinen11Institute of Biotechnology, Protein Chemistry Laboratory, University of Helsinki, Finland,2Norwegian College of Fishery Science,University of Tromsø, Norway,3Institute of Medical Biology, University...
- 8
- 428
- 0
Báo cáo Y học: Purification and characterization of three galactose specific lectins from Mulberry seeds (Morus sp.) ppt
... galactose.Protein and carbohydrate analysis The concentration of protein was measured by the method of Lowry et al. [13] using BSA as the standard. The presence of sugar in the protein was detected by periodic ... In the other side of the tank, a light source wasplaced in order to attract the nauplii. Two days were allowedfor the hatching of all the eggs and sufficient maturation of the nauplii for the ... by the Lowry method. The neutral sugar contents of the lectins, MSL-1, MSL-2, and MSL-3 were found to be 5.7, 5.4 and 4.5%, respectively. The sugar composition of the lectins as identified by...
- 6
- 475
- 0
Báo cáo Y học: Purification and characterization of VanXYC, a D,D-dipeptidase/D,D-carboxypeptidase in vancomycin-resistant Enterococcus gallinarum BM4174 docx
... VanX- and VanY-type enzymesindicated that all of these enzymes contained the same Zn2+binding motif. VanX homologues present in the cyanobac-terium Synechocystis strain PCC6803, and the glycopeptideantibiotic ... aD-Ala-D-Ser standard curve. Fordetermination of the rate of hydrolysis of UDP-MurNAc-pentapeptide[Ala], His6–VanXYCwas used at a concentra-tion of 2.25 · 10)7M. The rates of hydrolysis of 2, ... parameters of D59A for hydrolysis of UDP-MurNAc-pentapeptide[Ala]. However, its activitywas comparable with that of D59S and His6–VanXYC .The rates of hydrolysis of D-Ala-D-Ala and UDP-MurNAc-pentapeptide[Ala]...
- 7
- 414
- 0
Báo cáo Y học: Purification and characterization of two secreted purple acid phosphatase isozymes from phosphate-starved tomato (Lycopersicon esculentum) cell cultures ppt
... below.AP assaysAll assays were linear with respect to time and concentration of enzyme assayed. One unit (U) of activity is defined as the amount of AP resulting in the hydrolysis of 1 lmol of substrate ... °C.Phosphatase assay AFor routine measurements of AP activity, the hydrolysis of phosphoenolpyruvate to pyruvate was coupled to the lactatedehydrogenase reaction and assayed at 25 °C by monitoring the oxidation ... absence of exogen-ous Pihad only approximately 40% of the fresh weight of the 8-day-old +Picells (approximately 63 and 25 g of cellswere obtained per 500 mL culture of 8-day-old +Pi and –Picells,...
- 9
- 534
- 0
Báo cáo Y học: Puri®cation and characterization of the human adenosine A2a receptor functionally expressed in Escherichia coli doc
... determination of hA2aR by X-ray or electron crystallography, or infor-mation on the bound ligand conformation obtained byNMR spectroscopy, would assist in the design of subtypespeci®c compounds and ... cally to the XAC-agarose.Theophylline was removed by gel ®ltration before quanti-fying speci®c [3H]ZM241385 binding in the X AC-agaroseeluate. The value obtained was only 50±80% of the theoretical ... and amino-acid analysis. The sequence obtained, namely Lys-Ile-Glu-Glu-Gly-Lys-Leu-Val-Ile-Trp corresponds to the N-terminus of the m ature maltose-binding protein. Binding of the fusion protein to the IMAC...
- 11
- 582
- 0
Từ khóa: báo cáo y họcbáo cáo y học cổ truyềnmẫu báo cáo y học cổ truyềnbao cao y hoc colchicinphan ban luan trong bao cao y hoc co truyenbáo cáo khoa học y họcbáo cáo y tế học đườngmẫu báo cáo y tế học đườngbáo cáo y tế học đường cuối nămbáo cáo y tế học đường năm 2012báo cáo y tế học đường trường mầm nonbiểu mẫu báo cáo y tế trường họcbáo cáo y tế học đường trường tiểu họcbáo cáo y tế trường tiểu họcbáo cáo y tế trường học năm 2012Nghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngchuyên đề điện xoay chiều theo dạngMột số giải pháp nâng cao chất lượng streaming thích ứng video trên nền giao thức HTTPNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDEPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhPhát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longPhát hiện xâm nhập dựa trên thuật toán k meansNghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếNghiên cứu tổng hợp các oxit hỗn hợp kích thƣớc nanomet ce 0 75 zr0 25o2 , ce 0 5 zr0 5o2 và khảo sát hoạt tính quang xúc tác của chúngSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXGiáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtChiến lược marketing tại ngân hàng Agribank chi nhánh Sài Gòn từ 2013-2015TÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲ