Báo cáo khoa học: The phosphorylation pattern of human as1-casein is markedly different from the ruminant species potx

Báo cáo khoa học: Production and characterization of a thermostable L-threonine dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus docx

... increased by the addition of 2mm CoCl2(relative activity to that of the standardreaction 170%) and not by the addition of 2 mmZnCl2or one of the other metals ⁄ salts tested. The enzyme was ... this indicatesthat the metal ion is essential for activity, furtherresearch is needed to establish if the zinc atom is catalytic or structural. This has been done for the TDH of E. coli, and X-ray ... that the genome neighbourhood of the tdh homologs in the related species P. furiosus, Pyro-coccus abyssi and P. horikoshii is highly conserved.Interestingly, this analysis revealed that the...

Báo cáo khoa học: Expression and characterization of recombinant 2¢,5¢-oligoadenylate synthetase from the marine sponge Geodia cydonium ppt

... that the addition of the nuclease caused the precipitation of the material in aconcentration-dependent manner. Formation of the precipitate in the solution containing the highestamount of the ... different sponge cDNA constructs werechosen for studies investigating whether modiﬁcation of either the N- or C-terminus of the protein couldaffect the properties of the enzyme. For comparison, ... the calculatedpI of the recombinant 2-5A synthetase from G. cydo-nium is 9.6 [27]. Therefore, the protein should be pos-itively charged at neutral pH. However, the analysis of the protein preparation...

Báo cáo khoa học: Mechanisms and kinetics of human arylamine N-acetyltransferase 1 inhibition by disulﬁram potx

... than the reaction of cisplatinwith the enzyme, thus supporting the suggestion thatNAT1 could be an in vivo target of DS. The human NAT2 isoform shares a similar structure and mecha-nism of action ... thus be inhibited by the DS-depen-dent formation of an intramolecular mixed disulﬁde[3]. Therefore, the DS-dependent inhibition of NAT1 is probably the result of the formation of a stable DSadduct ... unlikely to be a result of the formation of a mixed disulﬁde, but rather of the formation of a stable DS adduct.DS reacts with NAT1 thiol groups at therapeuticconcentrationsDS is a drug that reacts...

Báo cáo khoa học: Differential expression pattern of the novel serine⁄threonine kinase, STK33, in mice and men doc

... rather reﬂects synchrony to otherevents of the spermatogenesis. For instance, the mech-anisms unleashing the pass of the haploid germ cellsthrough the tight junctions from the adluminal to the luminal ... spectrum of tissues but restric-tion to particular cell types and populations. The absence of signal in regions of the nervous system in the human cDNA dot-blot analysis and mouse nor-thern blot is ... the sig-nal is stronger in older mice.DiscussionHere we report the ﬁrst survey of the distribution of the recently discovered serine ⁄ threonine kinase 33 in mouseand human tissues. Our results...

Báo cáo khoa học: Molecular dynamics structures of peptide nucleic acidÆDNA hybrid in the wild-type and mutated alleles of Ki-ras proto-oncogene ppt

... sequence is because of the provenefﬁcacy of the designed PNA to down-regulate the gene expression of the mutated allelle, while sustaining the transcription of the wild-type allele [29,29a]. The present ... summary, the present study offers a possiblestereochemical basis for the experimentally observedhighly diminished stability of the PNAÆDNA duplex in the presence of A C mismatch vis-a`-vis DNA ... of the hydro-gen bond and water organization in the vicinity of the mismatch might be the contributing factors for the increase in free energy and diminished stability of PNAÆDNA vis-a`-vis...

Báo cáo khoa học: Secondary structure assignment of mouse SOCS3 by NMR deﬁnes the domain boundaries and identiﬁes an unstructured insertion in the SH2 domain pdf

... That the KIR is mostly unstructured when SOCS3 is in isola-tion is perhaps not surprising in view of the hypothesisfor its mechanism proposed by Yasukawa et al. [6].This requires the KIR to ... mutation. The PEST sequence identiﬁed in SOCS3 does notoccur, according to sequence analysis, in the samelocation in any other members of the SOCS family,apart from CIS. Analysis of the sequence of ... atotal of 290 lL of 80–200 lm of the gp130 peptide. The heat of dilution of the gp130 peptide into buffer was deter-mined in control experiments and subtracted from the rawdata of the binding...

Tài liệu Báo cáo khoa học: Two novel variants of human medium chain acyl-CoA dehydrogenase (MCAD) K364R, a folding mutation, and R256T, a catalytic-site mutation resulting in a well-folded but totally inactive protein pptx

... that the primaryeffect of this mutation is not on the catalytic activity of the enzyme.Absorption spectra of the puriﬁed mutantproteinsTo assess whether these mutations affected the afﬁnity of ... present on the west-ern blot and by the minimal effect of chaperonin(Fig. 1B). This suggests that the R256T mutationharms the function of the folded protein rather than the ability of the protein ... more complete picture of the bio-chemical outcome of these point mutations.Results The effect of chaperonin on the ability of the mutant proteins to foldFor a number of other MCAD point mutations,co-overexpression...

Tài liệu Báo cáo khoa học: Three-dimensional structures of thermophilic b-1,4-xylanases from Chaetomium thermophilum and Nonomuraea ﬂexuosa Comparison of twelve xylanases in relation to their thermal stability pdf

... part of the C-terminal tail of the full-length NFX with total 301 residues. The aminoacids of the C-terminal tail were sticking out from the model, but if the C-terminus of NFX is excluded, the enzyme ... [39]. These results showed that the introduction of ﬁvearginines into the Ser/Thr surface increased considerably the thermotolerance in the presence of the substrate.Another trend is that in thermophilic ... increase the stability of D. thermophilum xylanase [15,19]. Despite all thesestudies, the structural basis for the thermostability of family11 xylanases is not well understood.We report here the...

Tài liệu Báo cáo khoa học: Multiple enzymic activities of human milk lactoferrin ppt

... that thesefive activities are associated with different isoforms of LFand that, in addition to the three previously reportedisoforms [9], one of which is LF RNase, further isoforms of this protein ... Thedatashowtherelativeactivity of different subfractions of LF from the milk of one donor (Fig. 2) and the range of variation in the relative activities of LF subfractionspurified from milk of ... activities of LF in the hydrolysis of DNA,RNA, and ATP with those of other DNases, RNases, andATPases of human milk. Published data on these activitiesin human milk are limited. The1 4-kDa RNase...

Báo cáo khoa học: Folding and turnover of human iron regulatory protein 1 depend on its subcellular localization pdf

... clusterassembly machinery of yeast mitochondria can build the inorganic unit of this normally cytosolic protein.Furthermore, members of the extra-mitochondrialassembly line of these cofactors, the so-called ... cluster biosynthesis hasrecently been the topic of intense interest, particularlyin S. cerevisiae. An extensive set of proteins participa-ting in different stages of the process has been discov-ered. ... subunits of the KGDH complex were alsobound to these large structures, together with > 20other mitochondrial proteins [22]. However, the com-position of the aggregates in this study is far...

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