Báo cáo khoa học: Analysis of the effect of potato carboxypeptidase inhibitor pro-sequence on the folding of the mature protein pot

... (Eur. J. Biochem. 270) Ó FEBS 2003 Analysis of the effect of potato carboxypeptidase inhibitor pro-sequence on the folding of the mature protein Sı´lvia Bronsoms, Josep Villanueva, Francesc ... min; then 10 lLofincreasingconcentrations of PCI or ProNtPCI were added and the measures were continued for 2 min. The slope of the ﬁrst part of the assay corresponded to moand the slope of the ... folding of the mature protein and the dimerization of the molecule [12]. Incontrast, the studies performed with x-conotoxins demon-strated that the mature forms of these molecules containsufﬁcient...

Tài liệu Báo cáo khoa học: Analysis of the molecular dynamics of medaka nuage proteins by ﬂuorescence correlation spectroscopy and ﬂuorescence recovery after photobleaching doc

... respectively, in the part of the cytoplasmoutside of the granules. FRAP analysis in the gran-ules provided typical recovery curves of these fusionproteins: a diffusion constant with two components of 0.9 ... and to mea-sure the mobility of the components in the PGCs. The medaka embryo was peeled off the chorion, and the segment containing the part of PGCs was excised forobservation by microscopy ... Drosophila, the function of the nuage might berelated to the protection of the genome via repression of the selﬁsh genetic elements in the female germ line[3]. The nuage is known to be an electron-dense...

Báo cáo khoa học: Analysis of Lsm1p and Lsm8p domains in the cellular localization of Lsm complexes in budding yeast ppt

... localization. The N-terminushas the biggest effect on localization; the C-terminusplays a secondary role, determining the preferred local-ization in the absence of the N-terminus and the Smdomain ... understress conditions, showing that none of the Lsm1pdomains is required absolutely for this localization.However, a strong reduction in P-body localization of many of these mutant proteins shows ... for the localization and function of thesecomplexes.In budding yeast, only one form of the homologousSm complex exists; it forms the core of non-U6 spliceos-omal snRNPs and accumulates in the...

Báo cáo khoa học: Analysis of the NADH-dependent retinaldehyde reductase activity of amphioxus retinol dehydrogenase enzymes enhances our understanding of the evolution of the retinol dehydrogenase family pot

... (Sigma-Aldrich).Concentrations of ethanol in the reaction mixture did notexceed 1%. Retinoid concentrations were determined on the basis of the corresponding extinction coefﬁcients at the appropriate ... anchor the protein to the ER membranes. Furthermore, we analyzed the contribution of the C-terminal end to ER localization.We fused the last 41 amino acids of the amphioxusRDH2 enzyme (a region ... ª 2007 The Authors Journal compilation ª 2007 FEBSTo analyze the contribution of protein domains to the ER anchorage, the localization of the full-lengthenzyme was compared with those of ﬁve...

Báo cáo khoa học: "Analysis and Synthesis of the Distribution of Consonants over Languages: A Complex Network Approach" pptx

... work. Rather, here we try to investigate the ef-fect of the exact distribution of the language inven-tory size on the nature of the degree distribution of the consonants through a synthetic ... contributions, pointing out some of the implications of the current work and indi-cating the possible future directions.2 PlaNet: The Phoneme-LanguageNetworkWe deﬁne the network of consonants ... consists of 21 con-sonants which have a very high frequency (i.e., the degree k) of occurrence. Regime 2 of Fig-ure 3(b) also correspond to these 21 consonants. On the other hand Regime 2 of...

Báo cáo khoa học: Analysis of the regulatory motifs in eukaryotic initiation factor 4E-binding protein 1 pot

... that the introduction of acidic residues at the positions of the phosphorylationsites in 4E-BP1 decreases the interaction of 4E-BP1with raptor. This implies that the regions of 4E-BP1containing ... Somedegradation of the GST fusion proteins isevident from the presence of products run-ning at the position of GST itself. (E) Binding of raptor to different amounts of wild-type4E-BP1 or the AAAA ... proce-dures). The top sections of each panel show the blots for Myc-tagged raptor; the bottomsections show the blots with anti-GST toallow a comparison of the amounts of GSTfusion proteins used...

Báo cáo khoa học: Analysis of the transcarbamoylation-dehydration reaction catalyzed by the hydrogenase maturation proteins HypF and HypE pot

... the maturation of the enzymes. Most o f them a ct pleiotropicallyin the synthesis of all four hydrogenases, in particular in the synthesis and insertion of the metal centre [6,7]. Two of the products of the ... doi:10.1111/j.1432-1033.2004.04280.xcharacterization relative to the substrate ATP. The analysis of mutant variants of HypE demonstrates that the carb-amoylation of th e C-terminal thiol and the d ehydration of the S-carboxamide to the ... 10)3s)1. Analysis of the transcarbamoylation reaction catalyzedby HypF The formation of the HypE-thiocyanate involves ﬁrst the carbamoylation of the C -terminal c ysteine of HypE b yinteraction with...

Báo cáo khoa học: Analysis of the in planta antiviral activity of elderberry ribosome-inactivating proteins potx

... to conﬁrm the presence of the RIP/lectin codingsequence in the tobacco genome. Only those plants thatwere positive in the PCR analysis were withheld for further analysis. Expression of the ... Inhibition percentages were calculated by the followingformula: 100 · (number of lesions wild-type – number of lesionstransformant)/number of lesions wild-type. When the number of lesions of the ... At present, they are subdivided on the basis of the structure of the genes and the corresponding proteinsinto holo-RIPs and chimero-RIPs [3]. Whereas holo-RIPsconsist exclusively of a single...

Báo cáo khoa học: Analysis of the stability of the spermadhesin PSP-I ⁄ PSP-II heterodimer Effects of Zn 2+ and acidic pH pdf

... trans-ition temperature of the thermal denaturation. Thisdestabilization occurs with the dissociation of the het-erodimer at low protein concentrations. Nevertheless, the concentration of PSP-I⁄ ... presence of 3 mm CaCl2did not modify the chromatographic behaviour of PSP-I⁄ PSP-II. In con-trast, the addition of 2 mm Zn2+intensiﬁed the deviation of the elution proﬁle at low protein concentrations ... contrast, the results suggested the existence of an association-dissociation equilibrium leading to dissociation of the heterodimer at protein concentrations in the low nano-molar range.The...

Báo cáo khoa học: Analysis of the RNA degradosome complex in Vibrio angustum S14 potx

... equivalentamounts of each of these proteins in the four samples.Interestingly, Band 6, which corresponded to a bandexcised in the vicinity of the 380-kDa region of the ﬁrst dimension BN-PAGE separation, only ... determine whether the degradosome components seen in Fig. 2B were still present across the mass range. Band 6 was chosen on the basis of the apparent migra-tion of the PNPase trimer on the BN-PAGE ... 2010 The Authors Journal compilation ª 2010 FEBS 5163separated, the T18 and T25 domains are inactive, but the interaction of the hybrid proteins results in the functional complementation of adenylate...

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