... Weak organic acid stress inhibits aromatic amino acid uptake by yeast,
causing a strong influence of amino acid auxotrophies on the
phenotypes of membrane transporter mutants
Bettina E. Bauer
1,
*, ... overexpressing the Tat2p high a nity tryp-
tophan permease. Weak acid stress therefore inhibits uptake
of aromatic amino ac...
... behavior of a 36 nucleosome long array reconsti-
tuted on the tandem repeat of 5s DNA under torsional
stress [70]. The acquired data allowed the determina-
tion of several elastic parameters of ... summarize the available data and their impact
on our knowledge of both nucleosomal structure and the dynamics of
nucleosome and chromatin fiber assembly and organizatio...
... Ala25 of
chain A is close to Asn77 of chain B, then Asn77 of chain A is
close to Ala25 of chain B.
Chain A Chain B Hydrogen bonds
Ala25 Asn77, Arg80 AlaO–ArgNH1
AlaO–ArgND2
Asn26 His35, Arg80, Asn39 ... genomic DNA using the following primers:
forward, 5¢-CACCAAACCTTATACGATTGATAAGGCA
AAC-3¢; and reverse, 5¢-TTATTATTGGGCGTAAGCT
TCTAG-3¢. The construct was cloned directly into...
... sam-
ples was analysed as a percentage of HSP expression in the
positive controls using aphelionÒ image analysis software.
Statistical analysis
The nonparametric Mann–Whitney unpaired test was ... plates were
always placed at the centre of the cross-linker at a
distance of 13.5 cm (minus the height of the culture
dish) from the UV lamp. The apparatus calculates th...
... microsomal oxidation pathways was analyzed
by RT-PCR. In addition, glutathione peroxidase and catalase activities, as
well as thiobarbituric acid reactive substances, were examined. In VLCAD
knockout ... which may be
associated with a substantial risk of ROS-induced liver
damage.
Fasting is characterized by a considerable influx of
fatty acids into the liver. As a consequence...
... 281,
6520–6527.
37 Madhurantakam C, Rajakumara E, Mazumdar PA,
Saha B, Mitra D, Wiker HG, Sankaranarayanan R &
Das AK (2005) Crystal structure of low-molecular-
weight protein tyrosine phosphatase from ... theoretical relaxation data of the monomer and
the haddock model of the dimer were computed using
HydroNMR [49,50], and the dissociation constant of
the dimer (K
d
),...
... dis-
sociation constant of the radioligand, K
a
is the equilibrium
dissociation constant of WTX, and a is the cooperativity
factor between radioligand and WTX.
For the concentration response of [
35
S]GTP[cS] ... transmission. The
nicotinic AChRs (nAChRs) are activated by nicotine
and belong to the ligand-gated ion channel superfam-
ily, whereas muscarinic AChRs (mAChR...
... to the
cofactor and thus influence the anticoagulant activity
of APC. An influence of position 23 on the interaction
to APC substrates FVa and FVIIIa also cannot be
ruled out. The same reasoning ... R
max
is the binding at saturation or
maximum surface coverage, C corresponds to the injected
analyte concentration and K
D
is the equilibrium dissocia-
tion constant. We are...
... revealed
that both the kinetics of enzyme acylation (k
2
) and
deacylation (k
3
) are reduced by chemical glycosylation,
also as a function of the glycan molar content of the
conjugates (Table ... A
3
where A
1
, A
2
, and A
3
are the fractions of the fast, slow and
stable amide protons and k
HX,1
and k
HX,2
are the apparent
exchange rate constants for the fa...
... transported in
its closed conformation along MT toward the plasma
membrane. On reaching the membrane, Krit1 would
detach from MT and be captured by activated Rap1
and ICAP-1 on the plasma membrane ... toward the plasma
membrane. When reaching the membrane, Krit1 would detach
from MT and be captured by activated Rap1 and ICAP-1 on the
plasma membrane.
S. Be
´
raud...