... protein. J. Exp. Med. 187, 631–640.
Ó FEBS 2003 Structural diversity of LPS in H. in uenzae (Eur. J. Biochem. 270) 3167
Structural analysis of lipopolysaccharides from
Haemophilus
in uenzae
serotype ... representative
ofthediversitywithinthetypefcluster.
Previous studies of LPS from H. in uenzae have
resulted in a structural model consisting of a conse...
... have investigated the import-
ance of the amino acid sequence of a-helix F (hF) and the
connecting loop to s3A (hF/s3A-loop) for the rate of latency
transition. We grafted regions of the hF/s3A-loop ... from the amount
of PAI-1 required to inhibit half the uPA. The half-life of
PAI-1 was finally calculated from an exponential decay plot
of the data obtained. Generally, only...
... suggested to be linked to
early stages of PD pathogenesis [26]. Interestingly, in
our model, dopamine induces an increase of the actin
bundles regulator fascin 1 [27] and discordant changes
of two calcium-dependent, ... of
β
β
-Actin
α
-Synuclein
Fig. 1. Relative expression of a-synuclein in b-gal and a-syn cells in
response to dopamine (DA) treatment. Results are indicated as...
... actual binding site of serotonin cannot be inferred
from these results. Although serotonin can bind to the
active site binding locus (W82), binding to PAS cannot
be ruled out. If serotonin binds ... enzyme in its activating
capacity for neutral substrates (0.8–1.0 mm). The same
is true for the binding of tyramine to D70G, although
the affinity of D70G for tyramine is weaker than the...
... (Fig. 1). FI traces
obtained from thylakoids were composed of three well-
distinguished phases in terms of amplitude and half-
time (Table 1).
In contrast with the I peak observed in FI of intact
leaves, ... blocking electron transfer
from Q
A
to Q
B
[21,22], is of importance for modulating
the dynamics of PQ pool reduction and determining its
effect on FI kinetics as...
... spectra
obtained by subtracting the spectrum of porphyrin alone
from the spectra obtained in the presence of different
concentrations of the lectin are s hown in Fig. 2B. The
difference spectra ... Waals ’ interactions and stacking of
aromatic side chains with the porphine core of the
porphyrins, as observed in the jacalin–H
2
TPPS interaction,
most likely favor the bindin...
... concentration o f 50 lg of p rotein per IEF strip for
silver staining, hot Coomassie s taining, ÔStains-AllÕ labelling
or immunoblotting. T he following running conditions were
used: 60 m in at 1 00 ... e ffect of the
deficiency in dystrophin o n the expression of Ca
2+
-binding
proteins. The relatively unique combination o f the pI value
and molecular mass of individual 2D spo...
... the bin ding to the E-box.
Substitution of Arg65 for Ala abolished the interaction of
DEC1 with BMAL1 and the binding of DEC1 to the E-box.
However, substitution of His57 for Ala did not affect ... (BMAL1:112–626) did not affect the
binding of BMAL1 to DEC1; deletion of 235 amino acids
(BMAL1:236–626) slightly diminished the binding,
although a strong binding ability of BMAL1:23...
... This is indeed the case as can be concluded
from the structural model of the PTS1-binding
C-terminal domain of HpPex5p (Fig. 4). In this domain
three of the seven Trp residues of HpPex5p are found.
However, ... residues
254–568 of HpPex5p, corresponding to the C-terminal
half of the protein including all TPR repeats (Fig. 4).
Addition of the L2 peptide (dansyl-GSKL) was...
... consists of heterodimers of the Jun (e.g. cJun,
JunB, JunD) and Fos (e.g. cFos, FosB, Fra1, Fra2)
families of proteins. Different homologues combine to
form different heterodimers, which in turn ... results in a positive (unfavourable) net
contribution to the overall free energy of binding. DS
conf
arises from a reduction in conformational degrees of free-
dom of backbone...