0
  1. Trang chủ >
  2. Luận Văn - Báo Cáo >
  3. Báo cáo khoa học >

Báo cáo khoa học: The N-acetylglutamate synthase/N-acetylglutamate kinase metabolon of Saccharomyces cerevisiae allows co-ordinated feedback regulation of the first two steps in arginine biosynthesis potx

Báo cáo khoa học: Energetic and metabolic transient response of Saccharomyces cerevisiae to benzoic acid docx

Báo cáo khoa học: Energetic and metabolic transient response of Saccharomyces cerevisiae to benzoic acid docx

... mechanisms is shown in Fig. 1. The fact that the presence of benzoic acid introducesan independent ATP drain in cell metabolism may alsobe of interest to those studying the regulation of cellenergetics ... particularly during the transient.During the entire observation period of 72 h after the shift in benzoic acid concentration, the increase in OUR and the decrease in biomass concentration in the chemostat ... that the benzoate transporter is induced within the first 3000 s of the transient response. In order to verify thishypothesis further, measurement of transcript and pro-tein levels during this...
  • 15
  • 380
  • 0
Tài liệu Báo cáo khoa học: The Arabidopsis protein kinase Pto-interacting 1-4 is a common target of the oxidative signal-inducible 1 and mitogen-activated protein kinases docx

Tài liệu Báo cáo khoa học: The Arabidopsis protein kinase Pto-interacting 1-4 is a common target of the oxidative signal-inducible 1 and mitogen-activated protein kinases docx

... alone. The kinase- deficient mutant,OXI1K45R, in which the lysine residue of the ATP bind-ing domain is mutated to arginine, still interacted withPTI1-4. These data indicate that the kinase ... tenth of the input was loaded on to the geland represents the amount of HIS-tagged proteins used for the assay. The in vitro binding assays were repeated twice usingrecombinant proteins prepared ... (A)Recombinant kinase- inactive GST-lofMPK3 and GST-lofMPK6 weremixed with HIS-OXI1 in kinase buffer and [c-32P]-ATP. (B) Recombi-nant kinase- active GST-MPK3 and GST-MPK6 or recombinant kinase- inactive...
  • 11
  • 700
  • 0
Báo cáo khoa học: The human pyridoxal kinase, a plausible target for ginkgotoxin from Ginkgo biloba docx

Báo cáo khoa học: The human pyridoxal kinase, a plausible target for ginkgotoxin from Ginkgo biloba docx

... tooverconsumption of Ginkgo seeds, which are the mainsource of ginkgotoxin [1]. In addition to the seeds,which accumulate the toxin, ginkgotoxin has also beenfound in the leaves of Ginkgo biloba ... the antivitamin character of both compoundsand explains a depletion of cofactor formation in the organism in the presence of the antivitamins. The kinetic data (Table 1) are a reflection of the structural ... comparison of the vitamin B6concentration in human blood and the gin-kgotoxin concentration in human blood after ingestion of Ginkgo remedies. The maximum daily intake of gin-kgotoxin in remedies...
  • 10
  • 530
  • 0
Báo cáo khoa học: The variable C-terminal extension of G-protein-coupled receptor kinase 6 constitutes an accessorial autoregulatory domain ppt

Báo cáo khoa học: The variable C-terminal extension of G-protein-coupled receptor kinase 6 constitutes an accessorial autoregulatory domain ppt

... relationships of the C-terminus of mGRK6To examine the functional significance of the C-ter-minus of mGRK6-A, a mutant was generated(mGRK6-A M1) carrying serine residues instead of the cysteine residues ... loss of the ability of the protein tophosphorylate light-activated rhodopsin. Interestingly,gradual removal of the C-terminal-most 16 residues of mGRK6-A M1 affected the activity of the proteintoward ... that both the N-terminal and the C-terminal phospholipid-binding sites of mGRK6-Care involved in the interaction of this mGRK6 variantwith phospholipid vesicles containing PtdInsP2. Theseobservations...
  • 13
  • 424
  • 0
Báo cáo khoa học: The N-acetylglutamate synthase/N-acetylglutamate kinase metabolon of Saccharomyces cerevisiae allows co-ordinated feedback regulation of the first two steps in arginine biosynthesis potx

Báo cáo khoa học: The N-acetylglutamate synthase/N-acetylglutamate kinase metabolon of Saccharomyces cerevisiae allows co-ordinated feedback regulation of the first two steps in arginine biosynthesis potx

... FEBS 2003 The N-acetylglutamate synthase/N-acetylglutamate kinase metabolon of Saccharomyces cerevisiae allows co-ordinated feedback regulation of the first two steps in arginine biosynthesis Katia ... (bothexpressing the kinase protein) allow growth of the arginine- deficient mutant in the absence of arginine. On the otherhand, plasmids pYK7 and the empty vector pTrc99a (bothlacking the yeast kinase ... ornithine synthesis. The feedback regulation of these first two steps in the arginine pathway has been clearlydemonstrated in the bacterium Pseudomonas aeruginosa and in two ascomycetes: S. cerevisiae...
  • 11
  • 313
  • 0
Tài liệu Báo cáo khoa học: The antibacterial and antifungal properties of trappin-2 (pre-elafin) do not depend on its protease inhibitory function pptx

Tài liệu Báo cáo khoa học: The antibacterial and antifungal properties of trappin-2 (pre-elafin) do not depend on its protease inhibitory function pptx

... kDa in nonreducing SDS ⁄ PAGE gel, indicating the homogeneity of the preparation.Antibacterial assays The antibacterial activity of the proteins was investigatedusing log-phase bacteria first ... oryeast cells in phosphate buffer containing 0.3 m NaCl.Heparin binding assays The heparin-binding capacities of trappin-2 and elafinwere assessed by affinity chromatography using heparin-Sepharose. ... which cleaves the Lys-Ala pep-tide bond between the N-terminal cementoin domainand the C-terminal elafin domain very efficiently in vitro [7]. The 38 residue N-terminal domain of trap-pin-2 has a...
  • 13
  • 610
  • 0
Tài liệu Báo cáo khoa học: The structure and biological characteristics of the Spirochaeta aurantia outer membrane glycolipid LGLB pdf

Tài liệu Báo cáo khoa học: The structure and biological characteristics of the Spirochaeta aurantia outer membrane glycolipid LGLB pdf

... sequence and branchinginformation [31]. The charge state of a fragment ion is thenidentified by u sing the isotope profile, owing to the highresolution provided by the TOF mass analyser. The product-ion ... cm) in pyridinium-acetate buffer,pH 4.5 (4 mL of pyridine and 10 mL of AcOH in 1 L of water), and the eluate was monitored by a refractive indexdetector.Configuration experimentsFor determining ... the identification of lipopolysaccharides andimmunodeterminan ts in pathogenic strains of Haemophilis in u-enzae; application to clinical isolates. In Mass Spectrometry in Biology and Medicine...
  • 11
  • 632
  • 0
Tài liệu Báo cáo khoa học: The distinct nucleotide binding states of the transporter associated with antigen processing (TAP) are regulated by the nonhomologous C-terminal tails of TAP1 and TAP2 ppt

Tài liệu Báo cáo khoa học: The distinct nucleotide binding states of the transporter associated with antigen processing (TAP) are regulated by the nonhomologous C-terminal tails of TAP1 and TAP2 ppt

... substrateaffinity, the C-terminal tail of TAP2 induces a conformation in NBD2 that is nonpermissive for ATP binding. The ADP-binding state of TAP2 allows binding of ATP to TAP1. We hypothesize that peptide binding ... The resulting ADP-binding state of TAP2 and the C-terminaltail of NBD1 allow binding of ATP to TAP1. Peptidebinding leads to a change in the conformation of the TMD,which is transmitted via the ... In the resting state of TAP, the NBD1has a much higher binding activity for ATP than the NBD2,while the binding of ADP to the two NBDs is equivalent. Toattribute the different nucleotide binding...
  • 16
  • 407
  • 0
Báo cáo khoa học: The natural mutation by deletion of Lys9 in the thrombin A-chain affects the pKa value of catalytic residues, the overall enzyme’s stability and conformational transitions linked to Na+ binding pdf

Báo cáo khoa học: The natural mutation by deletion of Lys9 in the thrombin A-chain affects the pKa value of catalytic residues, the overall enzyme’s stability and conformational transitions linked to Na+ binding pdf

... thrombin show that the A-chain closely follows the contour of the catalyticB-chain, hinging the two interacting six-stranded bar-rel-like domains of the B-chain [17]. A well-structurednetwork of ... flexibility of their side-chainsto the solvent the smaller the intrinsic fluorescence of the enzyme. The smaller gain in the intrinsic fluorescence of DK9upon Na+concentration (9 vs. 18% in WT) may ... simulation of DK9thrombin in explicit water solvent supports the role of the A-chain in affecting the conformation and catalyticproperties of the B-chain, particularly in some insertionloops of the...
  • 11
  • 553
  • 0
Báo cáo khoa học: The metabolic role and evolution of L-arabinitol 4-dehydrogenase of Hypocrea jecorina potx

Báo cáo khoa học: The metabolic role and evolution of L-arabinitol 4-dehydrogenase of Hypocrea jecorina potx

... among the members of the Lad1 terminal clade but which arefunctionally different from those present in other SDHs, arelocated at the facing rims of the two domains of the proteinthat form the ... in polyol binding in the SDH [25] are also absolutely conserved in Lad1, and thus cannot determine the binding efficacy of hexitols and pentitols. On the other hand, many of the amino acids addressed ... However,evidence is missing so far that the enzyme is indeedresponsible for the metabolism of any of these polyols in vivo.Totestthis,wemadeuseofaH. jecorinarecombinant strain in which the lad1 coding region...
  • 9
  • 422
  • 0

Xem thêm

Từ khóa: báo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcbáo cáo khoa học nông nghiệpbáo cáo khoa học lâm nghiệpNghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngNghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt namđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhNghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Tìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinThơ nôm tứ tuyệt trào phúng hồ xuân hươngSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXChuong 2 nhận dạng rui roTăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtHIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀM