Báo cáo khoa học: Determination of the redox potentials and electron transfer properties of the FAD- and FMN-binding domains of the human oxidoreductase NR1 doc

... of the redox potentials and electron transfer properties of the FAD- and FMN-binding domains of the human oxidoreductase NR1 Robert D. Finn1, Jaswir Basran2, Olivier Roitel2, C. Roland Wolf1, ... and kinetic properties of NR1, and as we have shown for CPR and P450 BM3(23,24,28) the properties of the ﬂavin binding domains of NR1 are likely to mimic the redox properties of the domains in ... spectroelectrochemical titrations of the isolatedFAD/NADPH and FMN domains of NR1 enabled the determination of the midpoint reduction potentials for the oxidized/semiquinone (E1) and semiquinone/hydroquinone(E2)...

Tài liệu Báo cáo khóa học: Determination by electrospray mass spectrometry and 1H-NMR spectroscopy of primary structures of variously fucosylated neutral oligosaccharides based on the iso-lacto-N-octaose core doc

... Leadeterminant.As the C3aion is at m/z 690, this excludes the possibility of the other Fuc being at the Gal next to the subterminalGlcNAc. The position of the second Fuc 3-linked to the internal ... demonstrateour strategy of the combined use of ES-MS and NMR foranalysis of the core-branching pattern and full sequenceassignment of four oligosaccharides isolated from human milk. These are variously ... function.One of the early demonstrations of the role of carbo-hydrates in recognition was binding of the inﬂuenza virus tored blood cells via sialic acid [1], and later by work on the chemical basis of...

Báo cáo khoa học: Determination of the reopening temperature of a DNA hairpin structure in vitro pptx

... strand DNA due to the unpaired3¢-end of the primer, until the ÔGGÕ bases in the hairpin stemare freed by elevating the reaction temperature and pairedwith the ÔCCÕ bases at the 3¢-end of primer ... that the failure of the primer 2–DNA templatehybrid primer extension reaction was due to the effects of the hairpin structure on the process of primer 2annealing to the DNA template.Analysis of ... work, the reopening of the hairpin structure and the subsequent primerextensions were attempted by dividing the reaction intoaliquots, and incubating at different temperatures. The results of these...

Báo cáo khoa học: Determination of the metal ion dependence and substrate speciﬁcity of a hydratase involved in the degradation pathway of biphenyl/chlorobiphenyl pot

... Possibleroles of the cation include the activation of water for the hydration reaction and ⁄ or the stabilization of the anion transition state. In the latter case, if the sub-strate coordination to the ... the mechanism, the substratebinding site and the role of the metal cofactor in the enzyme.ResultsCloning, expression and puriﬁcation of BphH The bphH gene was inserted into the expression ... shown inTable 2. The Kmvalues were similar with either metalcofactor. On the other hand, the kcatvalue is higher by1.7-fold with Mg2+. This suggests that the nature of the metal ion has...

Báo cáo khoa học: Gene expression silencing with ‘speciﬁc’ small interfering RNA goes beyond speciﬁcity – a study of key parameters to take into account in the onset of small interfering RNA off-target effects potx

... in the test and refer-ence samples. The presence of the internal standard probesat different locations of the array allowed quantiﬁcation of the local background and evaluation of the array homoge-neity, ... into account in the normalization.Furthermore, in order to consider the purity and quality of the mRNA, a second normalization step was performed on the basis of the average of ﬂuorescence intensities ... of 81%, 66% and 69% forSREBF1 ⁄ siRNA1 and reductions of 79%, 78% and 71% for SREBF1 ⁄ siRNA2 in 143B, A549 and IMR-90 cells, respectively (Fig. 1A). Under these conditions, the effect of the...

Báo cáo khoa học: Determination of thioxylo-oligosaccharide binding to family 11 xylanases using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and X-ray crystallography pot

... is the concentration of the protein–lig- and complex, and [P] and [L] are the concentrations of the free protein and free ligand (i.e. inhibitor), respect-ively [68]. Expressed in terms of r, ... ras a function of the free ligand concentration and the ﬁt to Eqn (3) (solid line), obtained in the case of TRX I and S-Xyl5-Me. On the basis of the Kaobtained, one can calculate the binding free ... slope of –Ka.In the case of nonspeciﬁc binding (in our case, PL2 and PL3complexes), the number of ligands bound to oneprotein molecule is proportional to the concentration of free ligand. Therefore,...

Báo cáo khoa học: Tumour necrosis factor-a attenuates insulin action on phosphoenolpyruvate carboxykinase gene expression and gluconeogenesis by altering the cellular localization of Foxa2 in HepG2 cells pptx

... identiﬁcation of the func-tional domains of accessory factors HNF3 beta (hepaticnuclear factor-3 beta) and HNF4 and the necessity of proper alignment of their cognate binding sites. MolEndocrinol ... the respective indicated times. Cells in the absence of any of these were taken as the control (Con). Two micrograms of total RNA were reverse transcribed with random primers and the levels of ... cellular status in the presence and absence of insulin and ⁄ or TNFa. Figure 2A, B shows the effects of TNFa pretreatment on insulin action on the localization of Foxa2 and Foxo1 within the cell. Incu-bation...

Báo cáo khoa học: " Determination of Syntactic Functions in Estonian Constraint Grammar " docx

... attached to words. The tags indicate the inflectional and derivational properties of the word and the word class membership, the tags attached during the last stage of the analysis indicate ... functions. The underlying principle in determin- ing both the morphological interpretation and the syntactic functions is the same: first all the pos- sible labels are attached to words and then the ... rate is smaller than 2% then 80-88% of words becomes syntactically unambiguous. The error rate of parser is 1-4% depending on the ambiguity rate of input. The main goal of this work is to elaborate...

Báo cáo khóa học: Determination of modulation of ligand properties of synthetic complex-type biantennary N-glycans by introduction of bisecting GlcNAc in silico, in vitro and in vivo pot

... lectins in the case of the siglecs [72], wasnoted to affect binding properties. Compared with the solid-phase assays, the studies of cell binding allowed determination of the ligand properties of biantennaryN-glycans ... of the introduction of the substitu-tion. The results, expressed as median ﬂuorescence intensity and percentage of positive cells, reﬂected (a) the histogeneticorigin of the cell type and the ... providedfor a shift of the conformational equilibrium of the torsionalangle x of the C5–C6 bond of the a1–6 linkage between the two positions around 180 ° and )60 ° to the 180 ° positionfor the biantennary...

Tài liệu Báo cáo khoa học: Aldehydes release zinc from proteins. A pathway from oxidative stress⁄lipid peroxidation to cellular functions of zinc pptx

... zinc and MT protect the liver and the heartagainst the toxic effects of ethanol [45–47]. The aboveresults suggest that a critical aspect of the protectivefunction of MT is the scavenging of the ... none of the aldehydes affects the absorbance of PAR. The data for the reaction of MT with formalde-hyde were corrected for the absorbance changes in the absence of MT.Thiol reactivities of MT and ... (b-hydroxyacrolein). The following investigations focus on the effects of acetaldehyde and acrolein because of the relevance of these aldehydes for the biological effects of ingestedethanol and lipid peroxidation,...

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