Báo cáo khoa học: A hydrophobic segment within the C-terminal domain is essential for both client-binding and dimer formation of the HSP90-family molecular chaperone pptx
Báo cáo khoa học: A hydrophobic segment within the C-terminal domain is essential for both client-binding and dimer formation of the HSP90-family molecular chaperone pptx
... regionincluding the hydrophobic segment of the C-terminal domain is essential for both the client binding and dimer formation of the HSP90-family molecular chaperone and that the dimeric configuration appears ... A hydrophobic segment within the C-terminal domain is essential for both client-binding and dimer formation of the HSP90-family molecular chaperone Shin-ichi Yamada1,2, Toshio Ono2, Akio ... HSP90 molecular chaperone; and the other is in the C-terminal domain. Minami et al. [16] and Tanaka et al. [17] confirmed the existence of these respective client-binding sites in the N- and C-terminal...
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Tài liệu Báo cáo khoa học: A novel coupled enzyme assay reveals an enzyme responsible for the deamination of a chemically unstable intermediate in the metabolic pathway of 4-amino-3-hydroxybenzoic acid inBordetellasp. strain 10d doc
... [6,8,27] or toany other sequences available in FASTA and BLASTdatabase programs at the DNA Data Bank of Jap an.Recently, we reported the cloning and s equencing of the gene encoding 4-amino-3-hydroxybenzoate ... release of ammonia, are a key enzyme in the metabolic pathways of 2-amino phenol and its deriva-tives. However, little is known about the metabolic stepsthat lead to the release of ammonia and the ... the determination of released ammonia. The coupled enzymeassay revealed the mechanism of the deamination reaction and the subsequent metabolism, including the deaminationstep. The product formed from 4-amino-3-hydroxybenzoicacid...
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Tài liệu Báo cáo khoa học: A novel nuclear DNA helicase with high specific activity from Pisum sativum catalytically translocates in the 3¢fi5¢ direction docx
... tocreate a partial duplex. A substrate with 5¢ and 3¢ hangingtails (Fig. 5D) was used for purification and for most of the characterization unless stated otherwise. The structures of the various ... from the bottom of the tube and assayed for DNAhelicase activity. The distribution of helicase activity, position of the sedimentation coefficient and molecular mass markers are shown. The markers ... cross-react withantibodies against plant helicases including PDH45 and PDH65 and also against human DNA helicases I, II, III and IV (data not shown). ssDNA-dependent ATPaseactivity was present at a...
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Tài liệu Báo cáo khoa học: "A High-Accurate Chinese-English NE Backward Translation System Combining Both Lexical Information and Web Statistics" pdf
... Chinese-English backward translation, i.e., the original language of NE and the target language in translation is English, and the source language to be translated is Chinese. There are two important ... forward translation is eas-ier than backward translation. On the one hand, there is no unique answer to forward translation. Many alternative ways can be adopted to forward translate an NE from ... translating candidates on the Internet. Al-Onaizan and Knight (2003) adopt language models to generate possible candidates first, and then verify these candidates by web statistics. They achieve...
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Báo cáo khoa học: A novel prokaryotic L-arginine:glycine amidinotransferase is involved in cylindrospermopsin biosynthesis potx
... instructions.PCR amplification of cyrA was performed using primerscyrA-F (5¢-CATATGCAAACAGAATTGTAAATAGCT-3¢) and cyrA-R (5¢-CTCGAGAATAATGATGAAGCGA-GAAAC-3¢), which incorporated NdeI and XhoI restrictionsites, ... Mutat Res 472, 155–161.14 Li R, Carmichael WW, Brittain S, Eaglesham GK,Shaw GR, Mahakhant A, Noparatnaraporn N,Yongmanitchai W, Kaya K & Watanabe MM (2001)Isolation and identification of ... Asn300 and Asn302 are replaced by the smaller amino acids ala-nine and threonine. This results in a much larger activesite than in AGAT, and allows for binding of inosaminephosphate, the substrate of...
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Báo cáo khoa học: A novel metallobridged bis(b-cyclodextrin)s fluorescent probe for the determination of glutathione doc
... processes such as transport,protein synthesis, catabolism and metabolism [1]. Itcan also protect cells against reactive oxygen species and help them maintain an adequate intracellularredox status [2]. ... nmolÆL)1, according to the formula C = KS0⁄ S, where K = 3 (standard devia-tion = 1.36), obtained from a series of 11 reagentblanks, and S is the slope of the standard curve. The relative standard ... solution wasmeasured at 365 ⁄ 480 nm. The GSH content of the plasma was derived from the standard curve and the regression equation. The average recovery test was made using the standardaddition...
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Báo cáo khoa học: A DmpA-homologous protein from Pseudomonas sp. is a dipeptidase specific for b-alanyl dipeptides Hidenobu Komeda and Yasuhisa Asano docx
... substrates for BapA:(Gly)2(Gly)3, D-Ala-Gly, D-Ala-(Gly)2(D-Ala)2, D-Ala-L-Ala (D-Ala)3(D-Ala)4, L-Ala-Gly, L-Ala-(Gly)2(L-Ala)2, L-Ala-D-Ala, L-Ala-D-Ala-L-Ala,DL-Ala-DL-Asn, ... Then a sample of the enzyme solutionwas taken, and the remaining activity of BapA wasassayed with d-Ala-pNA as a substrate under standardconditions. The enzyme was most stable in the pHrange ... pref-erence for the d-configuration of Ala-pNa and alanin-amide as substrates by BapA was also comparable tothat exhibited by DmpA. The substrate specificity of BapA was however, different from that of...
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Báo cáo khoa học: "A Flexible Stand-Off Data Model with Query Language for Multi-Level Annotation" ppt
... with a capital letter.Markables are the carriers of the actual annota-tion information. They can be queried by means of string matching and by means of attribute-valuecombinations. A markable ... toeach other, we (like also Heid et al. (2004), amongothers) adopt this as a metaphor for expressing the sequential and hierarchical relations betweenmarkables, and we use a set of relation ... nominalattributes can have one of a (user-defined) closed set of possible values. The data model also supportsassociative relations between markables: Markableset relations associate arbitrarily many markableswith...
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Báo cáo khoa học: "A PARAMETERIZED APPROACH TO INTEGRATING ASPECT WITH LEXICAL-SEMANTICS FOR MACHINE TRANSLATION" doc
... and AC stand for culminator, and anti-culminator, respectively. lSBecause the focus of this paper is on the lexical-semantic representation and associated aspectual parameters, the de- tails ... tienda al llegar Maria. Either one of these sentences is an acceptable translation for (7)0). However, the same is not true of (7)(it): 7 (9) (i) Juan habfa ido a la tienda euando Maria lleg6. ... words themselves. 1°Because we are not discussing the realization of temporal information (i.e., the time relations between the matrix and adjunct events), an abbreviated form of the actual chart...
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Báo cáo khoa học: A thermoacidophilic endoglucanase (CelB) fromAlicyclobacillus acidocaldariusdisplays high sequence similarity to arabinofuranosidases belonging to family 51 of glycoside hydrolases ppt
... Xsa (BH1874); Bacillus subtilis ssp. subtilis str. 168: CAA61937,arabinofuranosidase 1; CAA99576, arabinofuranosidase 2; Bactero-ides ovatus: AAA50391, arabinosidase 1; AAA50393, arabinosidase ... AAK84947, arabinofuranosidase; Clostridium acetobutyl-icum ATCC 824: AAK81366, ORF CAC3436; Clostridium sterco-rarium: AAC28125, arabinofuranosidase; Cytophaga xylanolytica:AAC38456, arabinofuranosidase ... and arginine, whichwere reduced by 10.1% together compared with EGF and 6.4% in comparison with AbjA. On the other hand, CelBcontains a higher percentage of alanine and proline and of uncharged...
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