... efficiencycompared to isoforms 6-6, 3-6 and 3-3 (in decreasing order).The 3-6 isoform, with kinetic parameters between those of GST isoforms 3-3 and 6-6, seemed to have the properties of each of its subunits.ImmunoblottingFig. ... [18].N-Terminal amino acid sequencingOne of the seven anion exchange purified isoforms, which isrecognized by the three antisera used, was chosen for N-terminal amino acid sequencing. For this ... chromatography,leading to the distinction of isoforms. Their characterizationwas performed by studying the kinetic parameters (Vmax,Kmwith the commonly used substrate CDNB and GSH)and by performing immunoblotting...