Báo cáo khoa học: Identiﬁcation, cloning and characterization of two thioredoxin h isoforms, HvTrxh1 and HvTrxh2, from the barley seed proteome pot

... about the origins of HvTrxh1 and HvTrxh2, the occurrence of the thioredoxin h spots in protein extracts from dissected barley seeds was analysed by 2D gel electrophoresis(Fig. 1B). The three thioredoxin ... cloning and characterization of two thioredoxin h isoforms, HvTrxh1 and HvTrxh2, from the barley seed proteome Kenji Maeda, Christine Finnie, Ole Østergaard and Birte SvenssonDepartment of Chemistry, ... with HvTrxh1) and wheat TrxTa (90%identical with HvTrxh2). Recombinant HvTrxh1, HvTrxh2 and TrxTa were produced in Escherichia coli and puriﬁedusing a three-step procedure. The activity of the...

Báo cáo khoa học: Identiﬁcation of a novel thyroid hormone-sulfating cytosolic sulfotransferase, SULT1 ST5, from zebraﬁsh Molecular cloning, expression, characterization and ontogenic study ppt

... question is whether expression of the newlyidentified SULT1 ST5 correlates with the development of the thyroid hormone endocrine system of the zebra-fish. To gain insight into this, RT-PCR was ... toamplify the complete coding region of this novel zebrafishSULT. With zebrafish total RNA as the template and oligo(dT) as the primer, the first-strand cDNA was syn-thesized using the First-Strand ... significantlevel of expression of this novel zebrafish thyroid hormone-sulfating SULTat the beginning of the hatching period during embryogenesis, whichgradually increased to a high level of expression throughout...

Tài liệu Báo cáo khoa học: Identiﬁcation of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt

... block the interaction between the EAD and RNA polymer-ase II subunit [11,45]. The interaction between the EAD and RGG3 might inhibit the high-afﬁnity Htelobinding of RGG3.To gain further insight ... S3). The EAD domain therefore inhibited the high-afﬁnityHtelo binding of RGG3 and RBD. The ability of RGG3 and RBD to repress transcription activation byEAD raised the possibility that RGG3 and ... ellipticity and shifted the spectrum from a strong positive bandto 265 nm (Fig. 5), which is characteristic of the paral-lel form and consistent with the results of previous CDstudies [40–42]. These...

Tài liệu Báo cáo khoa học: Identiﬁcation and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebraﬁsh, Danio rerio docx

... Thisapproach was not used for stat6 as the region in whichthis gene was found in the zebrafish genome shared nosynteny with the human genome. The zebrafish gen-ome was searched using the human ... end of the gene, where the zebrafish and human sequences appearto be quite diverse. Together with the well-conservedgene organization, there is a conservation of syntenybetween the human and ... which two transcripts are pres-ent, that are important in the development of a number of these cell types.They were found within the zebrafish genome, using a synteny approach in the case of...

Tài liệu Báo cáo khoa học: Identiﬁcation and characterization of an R-Smad ortholog (SmSmad1B) from Schistosoma mansoni pdf

... genes from other species. For example, the loca-tion of the intron within the MH1-encoding region isconserved in the human Smad4 gene, whereas the intron within the linker-encoding region of SmSmad1Bis ... Smad3. The R-Smads of the BMP pathwayinclude Smads 1, 5 and 8. The type I receptor phos-phorylates the R-Smad at its C-terminal MH2 domain,causing the R-Smad to dissociate from the receptor. The ... postinfection, and there is a gradual decrease thereafter up to 21-day-old schistosomules, which represent the trough of the expression curves of both R-Smads. The levels thendisplay a slight increase,...

Tài liệu Báo cáo khoa học: Identiﬁcation, sequencing, and localization of a new carbonic anhydrase transcript from the hydrothermal vent tubeworm Riftia pachyptila docx

... reach the threshold for the RpCAbr tran-script in the branchial plume compared to the tropho-some whereas approximately ﬁve fewer cycles arerequired to reach the threshold for RpCAtr in the trophosome ... species, and other taxa, from hydrothermalvents and elsewhere. Biol Soc Wash Bull 6, 117–158.2 Childress JJ & Fischer CR (1992) The biology of hydro-thermal vent animals: physiology, biochemistry ... tran-script from the branchial tissue that is different from the one previously sequenced. In the present study, weshow that the two CA sequences are differentiallyexpressed in tissues of the worm. These...

Tài liệu Báo cáo khoa học: Identiﬁcation of b-amyrin and sophoradiol 24-hydroxylase by expressed sequence tag mining and functional expression assay docx

... identical to that of the authentic olean-3b,24-di-acetoxy-12-ene, except for the presence of several back-ground peaks (the amount of authentic sample wasadjusted to equalize the height of both peaks ... and characterization of the Arabidopsisthaliana lupeol synthase gene. Phytochemistry 49, 1905–1911.11 Shibuya M, Zhang H, Endo A, Shishikura K, KushiroT & Ebizuka Y (1999) Two branches of ... improve the biological production method, adetailed understanding of the biosynthesis of triterpenesaponins is required, including the enzymes catalyzing the sequence of reactions and the genes...

Tài liệu Báo cáo khoa học: Identiﬁcation of ATP-NADH kinase isozymes and their contribution to supply of NADP(H) in Saccharomyces cerevisiae docx

... 7). The deletion of YEF1 or POS5 from utr1 further decreased the growth of utr1 to the same level as that of the ftr1 mutant, which lacks ahigh-afﬁnity iron transporter and shows severe growthdefects ... and trimetaphosphate at 5 mm and polyphosphate, metaphosphate and hexametaphos-phate at 1 mgÆmL)1) and phosphorylated compounds(phosphocreatine, glucose-6-phosphate and phospho-Table 1. ... defects of the pos5 mutantTo conﬁrm the complementing ability of LEU2 for the growth defects of the pos5 mutant, we examined the growth of pos5 cells containing CgLEU2 instead of POS5 on the chromosome,...

Tài liệu Báo cáo khoa học: Identi®cation and properties of type I-signal peptidases of Bacillus amyloliquefaciens doc

... are distinguished by charge and hydrophobicitypro®le [3±5]. The N- and H -regions are t hought to interactwith the translocase machinery and to mediate membraneinsertion, whereas the C-region ... 2), and thencloned into the pQE16 vector. A fter restriction e nzymedigestion of p QE derivated plasmids (pQSh, pQTh, p QVh,pQWh, pQBh), the His-tagged genes (sipSh, sipTh, sipVh,sipWh, and ... stearothermophilus Sip1_Bst,Sip2_Bst(preliminary sequence d ata from the websitehttp://www.tigr.org). The l ength of e ach pair of branches represents the d istance betweensequence pairs and...

Báo cáo khoa học: Identiﬁcation of the amniotic ﬂuid insulin-like growth factor binding protein-1 phosphorylation sites and propensity to proteolysis of the isoforms docx

... possible that the chargerepulsion between the phosphate groups in the mid-region and the one located in the C-terminal portion of the protein may cause a conformational change thatmakes the cleavage ... increases the susceptibility to cleavage by the amniotic fluid pro-tease and suggest that not only the degree, but also the position of the phosphate groups influence the process.As for the other IGFBPs, ... that the nonphosphorylatedprotein, as well as the five distinct phosphoisoforms,were obtained in homogeneous form by anion exchangechromatography. Furthermore, the addition of the chelating agent...

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