Báo cáo khoa học: Limited proteolysis of Escherichia coli cytidine 5¢-triphosphate synthase Identiﬁcation of residues required for CTP formation and GTP-dependent activation of glutamine hydrolysis potx

Báo cáo khoa học: Limited proteolysis of Escherichia coli cytidine 5¢-triphosphate synthase. Identiﬁcation of residues required for CTP formation and GTP-dependent activation of glutamine hydrolysis potx

... 5¢-triphosphate synthase. Identiﬁcation of residues required for CTP formation and GTP-dependent activation of glutamine hydrolysis Dave Simard, Kerry A. Hewitt, Faylene Lunn, Akshai Iyengar and Stephen L. ... domain and wild-type ability tocatalyse NH3-dependent CTP formation. For these mutants,the values of kcat/Km and kcat for glutamine- dependent CTP formation were reduced % 20-fold and % ... NH3-dependent CTP formation in theabsence of ligands (s) and in the presence of ATP (10 mM, n), UTP(10 mM, h),andATPandUTPcombined(10mMeach, ,). Panel Bshows the inactivation of CTPS-catalysed glutamine- dependent...

Tài liệu Báo cáo khoa học: Catabolite repression in Escherichia coli – a comparison of modelling approaches docx

... (dFBA), and wasapplied for diauxic growth of E. coli on glucose and acetate [8]. The model predicts very well the timecourse of the external metabolites and the growth of biomass. In Santillan and ... system in Escherichia coli is a transport and sen-sory system and, in this function, is one of the key players of cataboliterepression. Mathematical modelling of signal transduction and gene ... phos-photransferase system (PTS) in Escherichia coli is one of the best studied biochemical networks from theo-retical and experimental points of view, and hasKeywords Escherichia coli; model veriﬁcation;...

Báo cáo khoa học: EspB from enterohaemorrhagic Escherichia coli is a natively partially folded protein pptx

... biological molecules. Knowledge of theconformational properties of EspB may clarify the role of EspB in bacterial attachment, but no informationabout the structural properties of EspB is currentlyavailable.In ... less organ-ized conformations of EspB and IpaC may be a com-mon property for this class of proteins.Importantly, some proteins that are natively unfol-ded show dramatic conformational changes ... interacts directly withthe translocated intimin receptor, Tir, of enteropatho-genic Escherichia coli, and is essential for pedestal formation. Cell Microbiol 3, 745–751.10 Vlademir VC, Takahashi...

Tài liệu Báo cáo khoa học: Limited suppression of the interferon-b production by hepatitis C virus serine protease in cultured human hepatocytes pptx

... observed that both T-pIC and M-pIC treatments induced the phosphorylation atSer386 and Ser396 of IRF-3, and formed the dimeriza-tion of IRF-3 (Fig. 5A,B, lanes 1 and 2), and thatNS3-4A remarkably ... Grants-in-Aid for the Third-Term Comprehensive10-Year Strategy for Cancer Control, and by aGrant-in-Aid for Research on Hepatitis, both from theMinistry of Health, Labor, and Welfare of Japan.References1 ... sequences of sense and antisense primers for RIG-I (accession no. NM_014314)were 5¢-AATGAAAGATGCTCTGGATTACTTG-3¢ and 5¢-TTGTCTCTGGGTTTAAGTGGTACTC-3¢. The seq-uences of sense and antisense primers for...

Báo cáo khoa học: Limited mutagenesis increases the stability of human carboxypeptidase U (TAFIa) and demonstrates the importance of CPU stability over proCPU concentration in down-regulating ﬁbrinolysis doc

... with (i) a mixture of A115 and F23, (ii) a mixture of A115 and F23, (iii) Y353 and (iv) A115.Table 5. Kinetic and stability parameters for puriﬁed WT and mutant CPUs. The T1 ⁄ 2 of activity at 37 ... in its name stands for unsta-ble [10,11]. The inherent and irreversible decay of CPU’s activity is believed to be of major importance for its in vivo down-regulation of activity and has beenlinked ... instability of CPU is likely to be of majorimportance for the in vivo down-regulation of its activity, but the under-lying structural mechanisms of this fast and spontaneous loss of activity of CPU...

Báo cáo khoa học: Endosomal proteolysis of diphtheria toxin without toxin translocation into the cytosol of rat liver in vivo pdf

... bond(Arg193–Ser194) and a disulﬁde bridge (Cys186–Cys201). Proteolytic nicking of the polypeptide and reduction of the disulﬁde are required for the A and Bfragments to separate and for cytotoxicity ... mobilities of the immunoreactive proform ( 64 kDa) and mature form ( 45 kDa) of cathepsin D, and soluble trun-cated furin ( 70 kDa). (B) Eluted fractions (5, 7, 9, 10 and 12) were tested for their ... administration of mDT, which displays a singlesubstitution of Glu52 for Gly in the A-chain, thekinetics of association with hepatic endosomes of T. El Hage et al. Activation and translocation of diphtheria...

Tài liệu Báo cáo khoa học: NirF is a periplasmic protein that binds d1 heme as part of its essential role in d1 heme biogenesis pdf

... which aligns with His200 of the d1heme domain of cd1, is essential both for this binding and for theproduction of d1heme; replacement of His41 by Ala, Cys, Lys and Met allgave nonfunctional ... information (Doc. S1, S2 and S3).Cloning of P. pantotrophus nirF and nirF variantsThe nirF ORF was ampliﬁed from P. pantotrophus genomicDNA using SB5 and SB6, digested with NcoI and XhoI and ... F and M. J. W. Dr AndreasSchlueter and Alfred Phu¨ler at the University of Biele-feld are thanked for their kind gift of plasmid pMS255.Dr Katalin di Gleria at The Weatherall Institute of Molecular...

Tài liệu Báo cáo khoa học: High-afﬁnity ligand binding by wild-type/mutant heteromeric complexes of the mannose pptx

... high-afﬁnitybinding of Man-6-P-bearing ligands, but possibly not for IGF-II. The present study, employing solubleforms of the receptor [25,26,34], indicates that theectodomain of the receptor is capable of ... ectodomain of these heterodimers,resulted in the predicted amount of ligand binding.This loss of binding function observed with asym-metric heterodimers may be due to deformation of thedimeric ... side of the dimer. Overall, theanalysis of IGF-II binding in such receptor dimerssuggests that each half of the dimer is capable of binding IGF-II independently of the ligandoccupancy of the...

Tài liệu Báo cáo khoa học: Collagen I regulates matrix metalloproteinase-2 activation in osteosarcoma cells independent of S100A4 pdf

... interact with twotypes of collagen I found in vivo, and the form of thecollagen determines the cells synthesis and activation of MMP-2 as well as the synthesis of MT1-MMP and TIMP-1.Previously, ... whichsynthesis and activation of proMMP-2, as well as syn-thesis of MT1-MMP, TIMP-1 and TIMP-2, areaffected by the interaction of the cell with various bio-logical forms of collagen I. A ... autoactivation of MMP-9 to the 80 kDa inac-tive intermediate and the 68 kDa active species, whereTIMP-1 prevented the formation of the latter species[15,38,39]; APMA-induced autoactivation of...

Tài liệu Báo cáo khoa học: The most C-terminal tri-glycine segment within the polyglycine stretch of the pea Toc75 transit peptide plays a critical role for targeting the protein to the chloroplast outer envelope membrane ppt

... theintermediate and mature forms of Toc75 in a givensupernatant or pellet fraction showed a similar pattern of protease sensitivity (e.g., compare lanes 4 and 6, or27 and 29 of Fig. 2A) as was shown before ... for AGA, compare lanes 19 and 20, 23 and 24, 27 and 28, and 31 and 32, respectively). Furthermore, trypsin-sen-sitivity of imported proteins was also consistent overthe time course (e.g., for ... low activity of the Plsp1 homolog that isresponsible for full maturation of Toc75 in the peaFig. 1. The biogenesis of pea Toc75. The precursor, intermediate, and mature forms of Toc75 are indicated...

Xem thêm