Báo cáo khoa học: No evidence for a role in signal-transduction of Na+/K+-ATPase interaction with putative endogenous ouabain potx

... concentra-tions of ouabain applied and (c) assume a similar role by putative endogenous ouabain or OLF. Ouabain- sensitive and -insensitive a- isoforms of Na+/K+-ATPase in ratsAll isoforms of ... chan-ges in intracellular Na+and Ca2+may thus be the result of a ouabain interaction with subpopulations of Na+/K+-ATPase [27]. An overall increase in intracellular Na+wasseen in ... concentrations of ouabain. Plasma concentrations of putative endogenous ouabain In another recent minireview in Eur. J. Biochem.[20]. endogenous ouabain was claimed to be a novel steroidhormone and the adrenal...

Báo cáo khóa học: FRET evidence for a conformational change in TFIIB upon TBP-DNA binding pptx

... case,whicharefusedtothetwoterminiofatargetprotein .With this structural constraint, it is fairly safe to assume that a change in FRET with CYIIB monitors a conformationalchange in TFIIB. Similar FRET-based conformationalindicators ... ratio attime t and at in nity, respectively. An error bar indicates theSD of each data point from the average value.ResultsDesign and biochemical integrity of CYIIBTo gain more insight into ... formation, TFIIB has been proposed to be a target of transcriptional activators [4–6]. Human TFIIB,consisting of 316 amino acid residues, is comprised of a N-terminal domain (NTD) that contains...

Báo cáo khoa học: Structural evidence for a constant c11 ring stoichiometry in the sodium F-ATP synthase doc

... enzymes significantly decreased the amount of the stable c11aggregates. A similar observation wasmade after incubating the sample for 5 min at 95 °CinSDS-containing loading buffer; this result ... c11 with 2 and 10 lgofc1purified in chloroform ⁄ methanol, the slowermigrating c ring aggregates did not reappear (lanes 6 and 7). Lane9, c1purified by extraction with chloroform ⁄ methanol. ... lanes, as indicated. Alsoshown is the nontreated c ring (–) and the c ring incubated at 95 °C for 5 min. The gel was stained with silver. A molecular mass stand-ard is shown.T. Meier et al....

Báo cáo khoa học: New evidence for the role of calcium in the glycosidase reaction of GH43 arabinanases pot

... andde-branched arabinans, and heteropolysaccharidessuch as arabinoxylans and arabinogalactans. Arabinanis composed of a- 1,5-linked l-arabinofuranosyl units,some of which are substituted with ... with a- 1,3- and a- 1,2-linked chains of l-arabinofuranosyl residues [4,5]. Twomajor enzymes hydrolyse arabinan: a- l-arabinofurano-sidases (AFNs; EC 3.2.1.55) and endo-1,5 -a- l-arab-inanases (ABNs; ... and arabinogalactan [6,7]. ABNs attack theglycosidic bonds of the a- 1,5-l-arabinan backbone,releasing a mixture of arabinooligosaccharides and l-arabinose [4]. These types of enzyme have attractedmuch...

Báo cáo khoa học: Biochemical evidence for conformational changes in the cross-talk between adenylation and peptidyl-carrier protein domains of nonribosomal peptide synthetases ppt

... thank Martin Hahn and Mohamed Marahiel for the kind gift of the sulfamoyl inhibitors, Helena Jan-zen for help with statistical analysis and Tulika Dhar for proof-reading of the manuscript. Funding ... sulfamoyl adenosine inhibitorsinduce conformational changes in the A- PCPprotein that are comparable to the effect of thenative substratesTo investigate conformational changes in a catalyti-cally ... additionalpresence of AMP and the nonhydrolyzable ATPanalog adenosine-5¢-[ (a, b)-methyleno] triphosphate(ApCpp) (data not shown). A comparison of the effect of compound 1 with that of the natural substrates ATP...

Tài liệu Báo cáo khoa học: Experimental proof for a signal peptidase I like activity in Mycoplasma pneumoniae, but absence of a gene encoding a conserved bacterial type I SPase pdf

... at its N-terminusinstead of a free amino group [32]. Therefore, theasparagine in position 26 was labeled within the intactprotein at its a- amino group and represents the aminoterminal amino ... Experimental proof for a signal peptidase I like activity in Mycoplasma pneumoniae, but absence of a geneencoding a conserved bacterial type I SPaseIna Catrein, Richard Herrmann, Armin Bosserhoff and ... Cleavage into P40 and P90 takes place after amino acid454. The N-terminus of the mature P40 starts with amino acid 26.The molecular mass of about 36 000 Da of P40, as determined bySDS ⁄ PAGE...

Báo cáo khoa học: "Personalized Normalization for a Multilingual Chat System" doc

... One of the major challenges for multilingual chat realized through machine translation technology is the normalization of non-standard, self-created short-forms in the chat message to standard ... effective approach for chat normalization. We express normalization using a probabilistic model as below )|(maxarg csPssbest and define the probability using a linear combination of features ... irregular and hard to predict their standard forms using morphological and phonetic similarity. It is also hard to train a statistical model if training data is not available. We asked the...

Báo cáo khoa học: "ENGLISH GENERATOR FOR A CASE-LABELLED DEPENDENCY REPRESENING" pdf

... a~ : If the partially instantiated template is for a noun phrase, apply the processor which, given an existing ,~3minal representation, instantiates a corresponding phrasal ~Iplate, to each ... syntactically legitimate variations on a giveJ, utterance, and has built in facilities to do some synon~s substitution. It has been used in a nu, ber of application domains: notably as a part ... are obviously interrelatea, each is distinct and separate. This well defzned separation greatly 194 increases t/~e extensability and maintainability of the syst~. A~ noted in the previous...

Báo cáo khoa học: "Knowledge acquisition for a constrained speech system using WoZ" pptx

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Báo cáo khoa học: "VOCAL INTEILFACE FOR A MAN-MACHINE DIALOG " doc

... single boards (Li~nard and Mariani, 1982 ; Gauvain, 1983 ; Asta and Li~nard, 1979) ; in our laboratory, such modules have been integrated into a compact unit that forms an autonomous vocal processor ... which has applications in a number of varied domains : vocal command of cars, of planes, office automation and computer-aided learning (N~el et al., 1982). Whereas most of the present language ... dialog-handling module easily adaptable to various domains of application. D 1 MACHINE Figure 6. Multibus configuration of the Vocal Terminal Acknowledgements We are particulary grateful...

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