... reverse, 5¢- GACCTTCTTCTCCCGCATCATC- 3¢. VEGF: forward, 5¢- ACGAAAGCGCAAGAAATCCC- 3¢; and reverse, 5¢- TTAACTCAAGCTGCCTCGCC- 3¢. Beta-actin: forward, 5¢- AGGCTCTTTTCCAGCCTTCCT- 3¢; and reverse, 5¢- GTCTTTACGGATGTCAACGTCACA- 3¢. Xenograft ... discriminateaggregates.siRNA-mediated knockdown of HIF- 1a and VHLexpression The DNA sequence corresponding to the targeting siRNAsis: HIF- 1a: 5¢- TCGACAAGCTTAAGAAAGA- 3¢; VHL: 5¢- CCAAGACACCTCGAGAAT- 3¢ (Ribobio, CN). The scrambled ... dilution; Cell Signaling Technology), visualizationwas achieved with enhanced chemiluminescence (AmershamPharmacia Biotech, Piscataway, NJ, USA) using a Gene-Gnome chemiluminescence imaging and analysis...