... Hospital Oakland Research Institute,Oakland, CA, USA for performing G C/MS an alyses, and Pr ofessorWalter Miller, U niversity o f C alifornia, San Francisco for the gift ofN-62 StAR protein and StAR ... 20 and 1.25 U Taqpolymerase (Promega). T he mixture w as heated to 94 °C for 2.5 min, and then amplified for 30 cycles as specified:94 °C for 30 s (denaturation), 55 °C for 20 s (annealing), and ... AbC-1 (lane 3) and mouse S-91(lane 4) melanom as; placenta (lane 5). Theamount of protein loaded on gels was 5 and 1 lg for placenta (lanes 1 and 2, respectively) and 20 lg for the skin samples.Ó...