Báo cáo khoa học: Identiﬁcation of a novel thyroid hormone-sulfating cytosolic sulfotransferase, SULT1 ST5, from zebraﬁsh Molecular cloning, expression, characterization and ontogenic study ppt

... 5¢-GAATTGGCCCTAATTTGCACATTAAAGATA-3¢Antisense: 5¢-GCCTGAAGTTTTTGGTTCACAGTGAAATTT-3¢ SULT1 ST4 Sense: 5¢-ACACTCTGAAGGGGAATTAGGATTAAGAAA-3¢Antisense: 5¢-CTGACTATACAAGGCTGTGTGCCACAAAAC-3¢ SULT1 ST5 Sense: 5¢-GAAAACACATCACGTACCCTCCCTCTCTGCG-3¢Antisense: ... 5¢-AGTTCAACAAGGAACTGCAGGACGTGTTTG-3¢Antisense: 5¢-CACATGGCTATAAAATGGTTACATCTGTGT-3¢ SULT1 ST2 Sense: 5¢-TATGTAGGAGCTACAAGAAACATTGAAGGC-3¢Antisense: 5¢-CAATTCTTACTAGCTGCAGGGAGGGTTGGT-3¢ SULT1 ST3 Sense: 5¢-GAATTGGCCCTAATTTGCACATTAAAGATA-3¢Antisense: ... sense (5¢-CGCGGATCCATGAGCCGGAGAACAAGCGAAACT-3¢) and antisense(5¢-CGCGGATCCTTATATAGTGAAGCGTATTGGAAG A novel zebraﬁsh cytosolic sulfotransferase S. Yasuda et al.3834 FEBS Journal 272 (2005) 3828–3837...

Tài liệu Báo cáo khoa học: Identiﬁcation of a novel matrix protein contained in a protein aggregate associated with collagen in ﬁsh otoliths pdf

... and the localization of Starmaker in zebraﬁsh. Dev GenesEvol 214, 582–590.26 Murayama E, Okuno A, Ohira T, Takagi Y & Nagasa-wa H (2000) Molecular cloning and expression of anotolith matrix ... byinvertebrates, and has three crystal phases: calcite, ara-gonite and vaterite. Although calcite is the most stablecrystal thermodynamically, many organisms can formmetastable aragonite crystals ... region wasdecreased and a new band was detected at 64 kDa, butonly after treatment with heparitinase II (Fig. 5A) .Although the same band was obtained after digestionFig. 3.45Ca overlay analysis...

Báo cáo khoa học: Identiﬁcation of a novel inner-core oligosaccharide structure in Neisseria meningitidis lipopolysaccharide docx

... 1000 (Table 1).Core oligosaccharide from the 1000 galE mutant strainwas also prepared and examined by MS. A range of molecular masses was found for the core oligosaccharideconsistent with a composition ... (1075), an additional phosphate group and an additional PEtn moiety (1155) and one additional phosphate group and two additional PEtn moieties (1278). Relative intensity of each glycoform/phosphoform ... region at 5.07 and 5.39 p.p.m. and a minorsignal at 5.50 p.p.m. were determined to be gluco-pyranose amino sugars, from the appearance of theirspin systems and the fact that the H-2 resonances of...

Báo cáo khoa học: Identiﬁcation of a novel binding site for calmodulin in ammodytoxin A, a neurotoxic group IIA phospholipase A2 docx

... CAAATACaAgCGGGtGAACGGGGCTATCGTCTGTGaAAAAGGC2– GGAATTCGCgGCcGCCtTGTCACACTCACAAATCCGATTCTC3+wtGGGCAAGCTTGCgGCcGCAATCTGCTTTCGAcAGAATCTGAAcACATAttcgAAAAAGTATATGC3+YIRNGGGCAAGCTTGCgGCcGCAATCTGCTTCCGAcAGAATCTGAAcACATACAgCTATATATATAGG3– ... TAATACGACTCACTATAGGGAGACCACAACGGTTTCC1– GGGCaagcTTCCCCGTCTCCtCCAGGATCATCtTCCCG2+ GGGCAAgcttgCTaTTcCCTCCTACTCCTcTTACGGATGCTACTGCGGCtgGGGGGG 2a+ GACTGCAaCCCCAAAtCGGACAG2b+ CAAATACaAgCGGGtGAACGGGGCTATCGTCTGTGaAAAAGGC2– ... GGAATTCGCgGCcGCCtTGTCACACTCACAAATCCGATTCTC3+wtGGGCAAGCTTGCgGCcGCAATCTGCTTTCGAcAGAATCTGAAcACATAttcgAAAAAGTATATGC3+YIRNGGGCAAGCTTGCgGCcGCAATCTGCTTCCGAcAGAATCTGAAcACATACAgCTATATATATAGG3– GGAATTCTGCAGTTAGCATTTgagCTCtccCTTGCACAAgAAGTCCGGÓ FEBS 2003 A novel CaM-binding site found in ammodytoxin A (Eur. J. Biochem. 270) 3019Vista, CA), digested with BamHI/HindIII...

Báo cáo khoa học: Identiﬁcation of a novel alternative splicing variant of RGS5 mRNA in human ocular tissues ppt

... for angiotensin II receptor (AT 1a) cDNAcloning: 5¢-CGCGGATGAAGAAAATGAAT-3¢ (forward);5¢-CCCTTTGGAAACTGGACAGA-3¢ (reverse).Primers used for cannabinoid receptor-1 (CB-1) cDNAcloning: 5¢-GAGGACCAGGGGATGCGAAGG-3¢;5¢-TGCCCCCTGTGGGTCACTTTCT-3¢.Plasmids ... Identiﬁcation of a novel alternative splicing variant of RGS5 mRNA in human ocular tissuesYanbin Liang, Chen Li, Victor M. Guzman, William W. Chang, Albert J. Evinger III, Dyna Sao and David ... RNA for RGS5 wasabundantly expressed in aorta, skeletal muscle, smallintestine and brain, and at low levels in heart, placenta,liver, colon, and leukocytes [9,10]. RGS5 acts as a potent GTPase...

Báo cáo khóa học: Identiﬁcation of a gene encoding Lon protease from Brevibacillus thermoruber WR-249 and biochemical characterization of its thermostable recombinant enzyme pptx

... werepurchased from Sigma.DNA manipulation and sequence analysisPlasmid DNA preparation, puriﬁcation of DNA from agarose gel, and restriction enzyme analysis were performedby the standard methods ... 11718–11728.27. Watanabe,S.,Muramatsu,T.,Ao,H.,Hirayama,Y.,Takahashi,K., Tanokura, M. & Kuchino, Y. (1999) Molecular cloning of theLonproteasegenefromThermus thermophilus HB8 and char-acterization of ... kDa), and carbonic anhydrase (29 kDa)] is shownas the standard curve. The molecular mass of native Bt-Lon (s)wasestimated from the standard curve based on the elution volume of native Bt-Lon and...

Báo cáo khoa học: Identiﬁcation of a putative triacylglycerol lipase from papaya latex by functional proteomics pdf

... 5¢-ACTCAAATCACTAGTATTCTTCCACCA-3¢ and 5¢-CATTTGAACATAAACATGAACAAATAAGTT-3¢ and the following conditions: annealing temperature 55 °C,25 cycles, Phusion polymerase used according to themanufacturer’s ... PhosphorImager. The percentage of release from position 2 was calculated as follows: area fatty acid ⁄ (area fatty acid +area lysoPtdCho). Pancreatic PLA2 was used as a positive control, and T. lanuginosus ... similarities withcastor bean acid lipase. Identiﬁcation and cloning of a putative lipase from C. papayaBecause V. heilbornii is a close relative of C. papaya, and that both expressed sequence tag...

Báo cáo khoa học: Identiﬁcation of a glycosphingolipid transfer protein GLTP1 in Arabidopsis thaliana ppt

... (5¢-AATAGAGAATTCAGAGAAAGAGATACGAGATGGA-3¢) and U660033Not (5¢-TCATAAGGCGGCCGCCTACATCGATCTTAATCTGCTCAA-3¢). A fragment carryingAt3g21260 cDNA was amplifed from A. thaliana RNA byRT-PCR. RNA was isolated from ... GLTP1PRON-BAM (5¢-CTCCTTGGATCCGCCTGAGAATTGAAAAAGGTGGG-3¢). A 1.3 kb fragment carrying the At1g21360promoter was ampliﬁed using primers 21360PRUXBA(5¢-AACGATCTAGATTAAGAATGTAATCACATTAGGGT-3¢) and ... activity assays A 1.8 kb DNA fragment carrying the At2g33470 promoterwas ampliﬁed from the A. thaliana Col-0 genome usingprimers GLTP1PROUXBA (5¢-AGACTGCTCTAGAATGGGTTTCTAAACCAACACGT-3¢) and GLTP1PRON-BAM...

Báo cáo khoa học: Identiﬁcation of a copper-repressible C-type heme protein of Methylococcus capsulatus (Bath) A member of a novel group of the bacterial di-heme cytochrome c peroxidase family of proteins docx

... GGCAACGAGCAAGGTCCGAAGsapE–R 2590 R GACGTCGTGAGTGCCTCCGTGmopB–F 3103 F CGACGTGCAGTATTACTTTTCTAGGGmopB–R 3103 R AGTATCAAACCGTGCTGGTCTCCHeme protein identiﬁcation in M. capsulatus O. A. Karlsen ... would lead to a mature protein of 732 amino acids with a theoretical molecular mass of 78 kDa. A search in the PROSITEdatabase of protein families and domains [12] withMCA2590 revealed two ... the supplied ‘RNAprotect BacteriaReagent Handbook’ with an additional DNase treatment onthe column. The RNA was dissolved in 50 lL of RNase-freewater and the A 260 and A 280values were determined....

Báo cáo khoa học: Identiﬁcation of a 250 kDa putative microtubuleassociated protein as bovine ferritin Evidence for a ferritin–microtubule interaction pdf

... Department of Bioscience and Bioinformatics, Kyushu Institute of Technology, Iizuka, Fukuoka, Japan2 Department of Biological Sciences, Faculty of Science, Kanagawa University, Hiratsuka, Kanagawa, JapanAlthough ... bovine adrenal gland ferritin; and lanes 3 and 6, the 250 kDa protein. Identiﬁcation of a putative MAP as ferritin M. R. Hasan et al.824 FEBS Journal 272 (2005) 822–831 ª 2005 FEBSabsorbance data ... puriﬁcation and partial characterization of a putative microtubule-associated protein (MAP) from bovine adrenal cor-tex with an approximate molecular mass of 250 kDa. The protein wasexpressed ubiquitously...

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