Báo cáo khoa học: Puriﬁcation and properties of the glutathione S-transferases from the anoxia-tolerant turtle, Trachemys scripta elegans pdf

Báo cáo khoa học: Puriﬁcation and properties of a new S-adenosyl-Lmethionine:ﬂavonoid 4¢-O-methyltransferase from carnation (Dianthus caryophyllus L.) pot

... 399-fold from the tissues of carnation (Dianthuscaryophyllus L). The enzyme, with a molecular mass of 43–45 kDa and a pI of 4.15, specifically methylates the hydroxy substituent in 4¢-position of the ... mL of the abovementioned culture medium: a total of 400 dishes wereprepared and used in the further steps of the experiments.Fungal materialFod pathotype 2 was used in the experiments, as the ... (Cs+ions of energy of 4 kV).Extraction and purification of F 4¢-OMTAll the purification steps were carried out at 4 °Ctempera-ture. The enzyme was concentrated at various steps of purification...

Báo cáo khoa học: Puriﬁcation and properties of the glutathione S-transferases from the anoxia-tolerant turtle, Trachemys scripta elegans pdf

... FEBS Puriﬁcation and properties of the glutathione S-transferases from the anoxia-tolerant turtle, Trachemys scripta elegans William G. Willmore and Kenneth B. StoreyInstitute of Biochemistry, Carleton ... MJ(2002) Comparative study of the puriﬁcation and char-acterization of the cytosolic glutathione S-transferases from two salmonid species: Atlantic salmon (Salmosalar) and brown trout (Salmo trutta). ... members of the bZIP family of transcription factors (Nrf2 and Maf G ⁄ K) bind [10]. The enzymes contain two bindingsites within the active site, a G-site for the binding of GSH and a H-site for the...

Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of glutamate N-acetyltransferase involved in citrulline accumulation in wild watermelon doc

... composed of two each of the a and b subunits.Analysis of the N-terminal transit peptide of CLGATAnalysis of the CLGAT cDNA predicted a chloroplasttransit peptide upstream from the N-terminus of CLGAT. ... clone of the GAT, gene-speciﬁc primers were designed from the sequence of watermelon EST AI563351 and used for 5¢- and 3¢-RACE. The cloned cDNA encoded a proteincomposed of 460 amino acids. The ... the puriﬁcation and characterization of GAT from wildwatermelon leaves, and discusses its function duringdrought ⁄ strong-light stress on the basis of its twounique enzymatic properties; thermotolerance...

Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of a sialic acid speciﬁc lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf

... constit-uents of the cell surface. They are found at the outermostends of the sugar chain of animal glycoconjugate. They actas an important component of the ligands recognized by the lectins. Recognition ... microtiter well and mixed. The values of the HAI titerwere determined after 60 min of incubation and expressed as the highest dilution of sugars that inhibited the agglutination of erythro-cytes. ... that purification of lectin from the hemolymph of crustaceans was most successful by affinitychromatography as it gave a higher fold of purification and percentage of recovery [20,24,27,35–39]. The...

Tài liệu Báo cáo khoa học: Identi®cation and properties of type I-signal peptidases of Bacillus amyloliquefaciens doc

... cloned into the vector pQE16 in frame with the His-tag-encodingsequence. The lepB gene was PCR ampli®ed from E. coliDNA using the primers Lep1 and Lep2 (Table 2), and thencloned into the pQE16 ... ABLASTsearch of the nucleotidesequence of the ampli®ed DNA fragment revealed the presence of three ORFs, the deduced proteins having 70, 77, and 67% identity to proteins encoded by the yhjE-sipV-yhjGTable ... extension, the forwardprimers CH1 and C H2 were designed for conservedregions HFGAGNIG and HGSAPDIAG of the genesmtlD and ycsA, which map in B. subtilis upstream of the searched sipU, and used...

Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of a membrane-bound enzyme complex from the sulfate-reducing archaeon Archaeoglobus fulgidus related to heterodisulﬁde reductase from methanogenic archaea pdf

... out in the presence of one of the substrates of the enzyme. The physiological electron acceptor of the A. ful-gidus enzyme is still unknown. Therefore, no substrate couldbe added to the titration ... enzyme complex from A. fulgidus (Eur. J. Biochem. 269) 1897Identiﬁcation of the genes encoding the subunits of the Hme complex and sequence analysis The N-terminal sequences of the four polypeptides ... Features of the subunits of the Hme complex from A. fulgidus. Data are eith er derived from the analysis of the sequence (calculatedmolecular mass, predicted transmembrane helices, cofactor binding...

Báo cáo khoa học: Puriﬁcation and characterization of zebraﬁsh hatching enzyme – an evolutionary aspect of the mechanism of egg envelope digestion pot

... sequences of ZP2 and ZP3 deduced from theircDNAs. The arrows and capital letters (sites A to F) indicate the cleaving sites of ZHE1 determined from the N-terminal amino acidsequences of the ZHE1 ... upstream from the cleaving site obtained from the 43 kDa product of ZP3(site E). Therefore, the ﬁnding that the 36.5 kDa prod-uct is a mixture of two peptides from ZP2 and ZP3suggested that the ... and E corresponded to the N-termini of the 39 kDaproduct of ZP2 and the 43 kDa product of ZP3observed in the 2 min ZHE1 digestion of unfertilizedegg envelopes, respectively. By contrast, the...

Báo cáo khoa học: Puriﬁcation and characterization of the cysteine proteinases in the latex of Vasconcellea spp. ppt

... Fractionation and purification of the enzymes stored in the latex of Carica papaya.J Chrom B 790, 229–238.32 Laemmli UK (1970) Cleavage of structural proteinsduring the assembly of the head of bacteriophage ... content in their latex; and (2) the pres-ence of other cysteine proteinases or isoforms in the latex. To investigate these two hypotheses, the proteinconcentration per milligram of dried latex, ... the other sequences. The MP tree reveals threemajor clusters. Cluster I combines the five chymo-papain isoforms of C. papaya with VXH-C, VS-B,CC-III and CC23. These results confirm the hypothesisof...

Báo cáo khoa học: Puriﬁcation and characterization of cathepsin B-like cysteine protease from cotyledons of daikon radish, Raphanus sativus docx

... CBCP from T. congolense is an atypical CBCP interms of its enzymatic properties. Therefore, TcoCB6 and daikon CBCP are distinct from one another interms of the role of the one histidine in the ... electrophoresis and N-terminal sequence of the purifiedenzyme. (A) Native PAGE of the purified enzyme (0.5 lg) in the absence of SDS. (B) SDS ⁄ PAGE of the purified enzyme (2 lg) and N-terminal sequence of bands ... occluding loop [13]. The affinity of the propeptide of cathepsin B for the loop-deletion mutant of cathepsin B was higher than for the wild-typeenzyme. As shown in Fig. 6, the increase in CBCP...

Báo cáo khoa học: Puriﬁcation and characterization of Helicobacter pylori arginase, RocF: unique features among the arginase superfamily doc

... After 1 h the reaction was stopped by the addition of 750 lL of acetic acid, and the color developedby the addition of 250 lL of ninhydrin (4 mgÆmL)1)at95 °C for 1 h. A standard curve of different ... Alabama Department of Microbiology and Immunology and the College of Medicine, by Public Health Service grant CA101931 (toD.J.M.) from the National Institutes of Health, and the AustralianResearch ... Additionally, there was significant variation in specificactivity from one batch of freshly purified arginase toanother, which could be a result of the instability of the enzyme during purification. The...

Xem thêm