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Báo cáo khoa học: Nuclear aggregates of polyamines are supramolecular structures that play a crucial role in genomic DNA protection and conformation potx

Báo cáo khoa học: Nuclear aggregates of polyamines are supramolecular structures that play a crucial role in genomic DNA protection and conformation potx

Báo cáo khoa học: Nuclear aggregates of polyamines are supramolecular structures that play a crucial role in genomic DNA protection and conformation potx

... aggregates of polyamines are supramolecular structures that play a crucial role in genomic DNA protection and conformation Luciano D’Agostino1, Massimiliano di Pietro1 and Aldo Di Luccia21 Department ... isthe charge attraction between DNA phosphates and the amino groups of polyamines. As the amino groups of polyamines are already engaged in ionic bonds withthe phosphates of NAPs, secondary amino ... believe that NAPs are the sole biological forms by which polyamines efficiently protect genomic DNA against DNase I, while maintaining its dynamic structure.AbbreviationNAP, nuclear aggregate of polyamines. FEBS...
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Báo cáo khoa học: The effect of replacing the axial methionine ligand with a lysine residue in cytochrome c-550 from Paracoccus versutus assessed by X-ray crystallography and unfolding ppt

Báo cáo khoa học: The effect of replacing the axial methionine ligand with a lysine residue in cytochrome c-550 from Paracoccus versutus assessed by X-ray crystallography and unfolding ppt

... changesbeing minimal the trapping of two main-chain confor-mations and increased B-factors at 295 K suggests that the presence of the coordinating Lys in some way can in uence the dynamics of ... indicate that dissociation of the K100 occurs in a similarmanner as for M100 coordination and that unlike atalkaline pH, unfolding using GdmHCl induces a ligand-exchange mechanism.DiscussionBacterial ... seemslikely that the mechanism of release of the axial ligandunder alkaline conditions and in the presence of GdmHCl is different. Under alkaline conditions release of the axial ligand is postulated...
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Báo cáo khoa học: Nuclear localization of human spermine oxidase isoforms – possible implications in drug response and disease etiology pptx

Báo cáo khoa học: Nuclear localization of human spermine oxidase isoforms – possible implications in drug response and disease etiology pptx

... Systems, San Diego, CA,USA) using PCR with the primer pair 5¢-CAATCCTCGAGTATGCAAAGTTGTGAATCCAG-3¢ and 5¢-TAATAAGCTTTGGTCCCCTGCTGGAAGAGGTC-3¢ and each cDNA in pET15b as the template. PCR products ... proximity to DNA, as well as decreases nuclear spermine levels, thus altering the protec-tive roles of spermine in free radical scavenging and DNA shielding, and resulting in an overall increased potential ... MD, USAThe naturally occurring polyamines, spermine, spermi-dine and putrescine, are polycations that are abundant and essential in both prokaryotic and eukaryotic cells.These molecules have...
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Báo cáo khoa học: Nuclear import of mPER3 in Xenopus oocytes and HeLa cells requires complex formation with mPER1 pdf

Báo cáo khoa học: Nuclear import of mPER3 in Xenopus oocytes and HeLa cells requires complex formation with mPER1 pdf

... Emery-LeM, Rosbash M & Allada R (2002) A role for caseinkinase 2alpha in the Drosophila circadian clock. Nature420, 816–820.19 Martinek S, Inonog S, Manoukian AS & Young MW(2001) A role for ... phosphoryla-tion and nuclear transfer of mPER3 [33]. Phosphoryla-tion of mPER1 itself correlates with nuclear transport[34]. Earlier studies had already indicated that nuclear translocation of mPER3 ... mPER1-NLS1,three of the basic amino acids were chan-ged to alanine (RRHHCRSKAKRSR). In mPER1DC and mPER1mutNLS1DC, the 186C-terminal amino-acid sequence containingNLS2 was deleted. myc mPER1 Frag 4a, aa...
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Tài liệu Báo cáo khoa học: PCR detection of nearly any dengue virus strain using a highly sensitive primer ‘cocktail’ ppt

Tài liệu Báo cáo khoa học: PCR detection of nearly any dengue virus strain using a highly sensitive primer ‘cocktail’ ppt

... CAGACTAGTGGTTAGAGGAGAGGAATGATGCTGTAGAGACADENV-1 92.1 ± 0.57 73.6 ± 2.311G4P217 ATATGCTGAAACGCGTGAGCATCATGAGACAGAGCGATDENV-3 90.3 ± 1.09 83.9 ± 5.161G5P30 TTCCAACAAGCAGAACAACATGCTACAGGCAGCACGGTTTDENV-4 ... Group 4 ATATGCTGAAACGCGTGAGCATCATGAGACAGAGCGATDENV-3 1043822791G5P30 Group 5 TTCCAACAAGCAGAACAACATGCTACAGGCAGCACGGTTTDENV-4 990310318415C. Gijavanekar et al. PCR detection of nearly any ... the broad strain sensitivity of thisreagent using DENV cDNA clones and RNA of thefour serotypes in the presence of a vast excess of human DNA and RNA. The reagent has high analyti-cal sensitivity...
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Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf

Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf

... Suzuki5 and Yasushi Kawata3,41 National Institute of Advanced Industrial Science and Technology, Ikeda, Osaka, Japan2 Department of Food Science and Nutrition, Faculty of Human Life and Science, ... Academia Sinica and National SynchrotronRadiation Research Center (Taiwan, ROC). Wethank Ms M.Sakai (Osaka University) for performingultracentrifugation analysis and Mr K. Mieda and M. Sakata (Tottori ... (cm) A BFig. 2. Assembly of ApeSOD chains in solution. (A) Representativegel filtration chromatogram of ApeSOD and calibration curve (inset).The standard proteins are albumin (1), ovalbumin (2),...
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Báo cáo khoa học: Kinetic characterization of methionine c-lyases from the enteric protozoan parasite Entamoeba histolytica against physiological substrates and trifluoromethionine, a promising lead compound against amoebiasis ppt

Báo cáo khoa học: Kinetic characterization of methionine c-lyases from the enteric protozoan parasite Entamoeba histolytica against physiological substrates and trifluoromethionine, a promising lead compound against amoebiasis ppt

... inhibitor was maintained at 1 : 1000. After1 h of preincubation, 2 mm Met was added, and theamount of a- keto acid was measured after 0, 12, 24 and 36 min, as described above. After the amount of a- keto-butyric ... transmethylation reactions, and the synthesis of polyamines, antioxidants, and cofactors [1]. As there are remarkable differences in trans-sulfurationpathways between organisms, these pathways, and in Keywordsamoebiasis; ... a halogenated analog of Met, has been exploited as a therapeutic agentagainst cancer as well as against infections by protozoan organisms and periodontal bacteria. However, its mechanism of action...
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Báo cáo khoa học: Possible involvement of an FKBP family member protein from a psychrotrophic bacterium Shewanella sp. SIB1 in cold-adaptation potx

Báo cáo khoa học: Possible involvement of an FKBP family member protein from a psychrotrophic bacterium Shewanella sp. SIB1 in cold-adaptation potx

... theNdeI–BamHI sites of pET-2 8a. This DNA fragment wasamplified by PCR. The sequences of the PCR primers were5¢-AGAGAGAATTCATATGTCAGATTTGTTCAG-3¢for the 5¢-primer and 5¢-GGCCACTGGATCCAACTACAGCAATTCTCA-3¢ ... mass of 28 kDa and an isoelectric point of four greatly increased at 4 °C compared to that at 20 °C.Determination of the N-terminal amino acid sequence, fol-lowed by the cloning and sequencing ... family member protein from a psychrotrophic bacteriumShewanellasp. SIB1 in cold-adaptationYutaka Suzuki, Mitsuru Haruki*, Kazufumi Takano, Masaaki Morikawa and Shigenori KanayaDepartment of...
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Báo cáo khóa học: Identification of domains on the extrinsic 23 kDa protein possibly involved in electrostatic interaction with the extrinsic 33 kDa protein in spinach photosystem II ppt

Báo cáo khóa học: Identification of domains on the extrinsic 23 kDa protein possibly involved in electrostatic interaction with the extrinsic 33 kDa protein in spinach photosystem II ppt

... structuraldeterminants present on the spinach 33 kDa protein that are required for the efficient binding of the 23 and 17 kDaproteins and that are absent in cyanobacterial and red algalproteins [24].The ... A ˚span) indicated that the 33 kDa protein is within a distance of 11 A ˚ of the 23 kDa protein and that the 23 kDa protein is within11 A ˚ of the 17 kDa protein [25]. This indicates that thesethree ... cyanobac-terial or red algal 33 kDa protein, could only partially rebindspinach 23 kDa protein but could not bind spinach 17 kDaprotein [23]. These data indicate that there are structuraldeterminants...
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Báo cáo khoa học: Crystal structure of heme oxygenase-1 from cyanobacterium Synechocystis sp. PCC 6803 in complex with heme pot

Báo cáo khoa học: Crystal structure of heme oxygenase-1 from cyanobacterium Synechocystis sp. PCC 6803 in complex with heme pot

... HO-1 and mammalian HO- 1 (in parentheses) are shown. Hydrogen bondingpatterns in the ÔopenÕ and ÔclosedÕ conformations and in the ÔmoreclosedÕ conformation are characterized by a- helix and p-helix,respectively.4520 ... T., Matsuno, A. , M uraki, A. ,Nakazaki, N., Naruo, K., Okumura, S., Shimpo, S., Takeuchi, C.,Wada, T., Watanabe, A. , Yamada,M.,Yasuda,M.&Tabata,S.(1996) Seque nce analysis of the genome of ... Yoshida, Yamaguchi, Japan;3Department of Biochemistry,Yamagata University School of Medicine, Yamagata, JapanHeme oxygenase (HO) catalyzes the oxidative degradation of heme utilizing molecular...
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