... to the following parameters: 94 °C for 4 min;10 cycles of 94 °C for 15 s, 52 °C for 30 s, 72 °C for 30 s; 20cycles of 94 °C for 15 s, 52 °C for 30 s, 72 °C for 30 s plus5 s at each cycle. The ... Pflanzenbiochemie,Halle) and Dr U. Arnold (Institut fu¨r Biochemie,Martin-Luther-Universita¨t Halle) for help with HPLC,AAS and RP-HPLC, respectively. Thanks to Dr A.Schierhorn (Max-Planck-Gesellschaft, ForschungsstelleEnzymologie ... Pyridine,metyrapone and different azole compounds generated type II binding spec-tra and the Kdvalues determined for these substances suggested thatP450mormight have a preference for more bulky and ⁄...