Báo cáo khoa học: The structural comparison of the bacterial PepX and human DPP-IV reveals sites for the design of inhibitors of PepX activity pot
Báo cáo khoa học: 9-Deazaguanine derivatives connected by a linker to difluoromethylene phosphonic acid are slow-binding picomolar inhibitors of trimeric purine nucleoside phosphorylase potx
... method, the inhibitor competeswith a high excess of substrate for the active sites of the enzyme; therefore, the slow-onset phase of the reaction may not always be observed [18]. This isshown in the ... time, the fluorescence of the solution maybe represented as the sum of the fluorescence of the variousmolecular species present in the mixture, free trimeric pro-tein, P, free ligand, L, and trimeric ... Con-centrations of the ligand varied in the range 0.05–6.4 lm.Concentrations of protein and ligand refer to the situationprior to mixing in the stopped-flow apparatus (i.e. to the concentrations in the...
- 14
- 368
- 0
Tài liệu Báo cáo khoa học: NMR structural characterization of HIV-1 virus protein U cytoplasmic domain in the presence of dodecylphosphatidylcholine micelles doc
... implications of the observed VpUcytstructureOne of the most studied activities of VpU is the induc-tion of CD4 degradation in the endoplasmatic reticu-lum of infected CD4+cells. Only the cytoplasmicdomain ... highlyattractive research topic and may be studied by solidstate NMR methods. However, the objective of the present study was to gather structural and dynamicdata of VpUcyt in the presence of membrane-simulat-ing ... of each cycle and provide additional guidance for the interpretation of the NOESY spectra in the subsequentcycle [78]. atnos revaluates the experimental NOESY datain each cycle [77]. The software...
- 16
- 632
- 0
Báo cáo khoa học: A structural overview of the PDI family of proteins docx
... strikingly different conformations in the structures of the isolated b¢ domain and full-lengthproteins. In the crystal structure of the b¢x fragment of human PDI, the C-terminal part of the x-linker turnsback ... Binding of PDI to the prolyl-4-hydroxylase complex minimally requires intactb¢ and a¢ domains of PDI, but the assembly and activ-ity of the complex is further enhanced by the addition of a and ... similarity to the a domains of other PDIs. Yellow boxescorrespond to the linker between the b¢ and a¢ domains (x-linker). The DnaJ domain of ERdj5 and the C-terminal helical domain of ERp29 are...
- 13
- 483
- 0
Báo cáo khoa học: A structural basis for the pH-dependence of cofilin F-actin interactions potx
... McGough and coworkers[20] and subsequently by others [31], and the thrustingforward of subdomain 2 with respect to the rest of the monomer.In this report we study the pH-dependence of the actin–cofilin ... controlledby the interplay of a variety of signaling pathways. The importance of the contribution of ADF/cofilins to cellmotility is reflected in their being regulated by many of thesesignals, ... to the sample(data not shown).Effect of pH on cofilin conformation The regulation of the cofilin activity by pH occurs in a pHrange suggesting the involvement of histidine residues. Infact, the...
- 8
- 409
- 0
Tài liệu Báo cáo khoa học: Phenol hydroxylase from Acinetobacter radioresistens S13 Isolation and characterization of the regulatory component docx
... the firsthypothesis and therefore a direct involvement of PHI inelectron transfer. This conclusion is consistent with the verylow degree of identity between the N-terminal sequence of PHI and ... onePHR and one PHO (abc) protomer. The hypothesis of adirect PHI–phenol interaction is quite unlikely, because of the fact that the addition of phenol does not alter the emission spectrum of PHI. ... bindANS (a probe for hydrophobic sites) and is not retained by the phenyl Sepharose column (a ligand for phenolic-recognizing sites and for hydrophobic sites in general).These results differ...
- 7
- 514
- 0
Tài liệu Báo cáo khoa học: Casein kinase 2 specifically binds to and phosphorylates the carboxy termini of ENaC subunits ppt
... between the C tails of b and c and the ubiquitin ligase Nedd4. The WW domains of Nedd4 bind to the proline-rich PY motifs of b and cENaC, leading to channel ubiquitination, internalization and degradation ... donor (Fig. 8A), the inhibitory effects of heparin and DPG (Fig. 8B), the fact that purified CK2 canphosphorylate bS631 and cT599 (Fig. 7A), the presence of the a subunit of CK2 in the active cytosolic ... fractions(Fig. 6), and the finding that the electrophoretic mobility of the phosphorylating kinase corresponds to that of the asubunit of CK2 (Fig. 9). However, other experimentsaiming to detect the b subunit...
- 8
- 345
- 0
Báo cáo khoa học: "A Practical Comparison of Parsing Strategies" docx
... prosecuted the queries on a remote computer, and responded with the requested information in a readable format tailored to the characteristics of the answer. The basis for the development of the ... determine whether they interact with the different parsers and/ or each other, whether any gains are offset by introduced overhead, and whether the source and precise effects of any overhead ... (instantiations of grammar rules) proposed by the parser; the number of these rejected by the rule-body procedures in the usual fashion; and the storage requirements (number of CONSes) of the analysis...
- 6
- 455
- 0
Báo cáo khoa học: A chloroplast RNA binding protein from stromal thylakoid membranes specifically binds to the 5¢ untranslated region of the psbA mRNA potx
... to +1157 relative to the AUG of the psbA mRNA(corresponding to the coding region of the C-terminalamino acids of D1 and the 3¢ UTR of the psbA mRNA) wasamplified with the oligonucleotides psbA3/1 ... used in the binding assays shown inFig. 3C) and the predominant, shorter form with a leader of 36 nucleotides (Fig. 5A) [34,35]. It has been hypothesizedthat the larger form represents the precursor ... found for the regulation of translation initiation of the psbD gene in chloroplasts of C. reinhardtii. In the case of the psbD 5¢ UTR, a U-rich element located 25 to 14nucleotides upstream of its...
- 8
- 338
- 0
Báo cáo khoa học: Ribonuclease H: molecular diversities, substrate binding domains, and catalytic mechanism of the prokaryotic enzymes ppt
... whether the enzymerequires one or two metal ions for activity, because the number of the metal-binding sites found in the crystalstructures of the enzyme–metal ion complex formed in the absence of ... absence of the substrate varies for differentenzymes and different metal ions. For example, singleMg2+[43] and Mn2+[44] ions bind to the active sites of the wild-type and mutant proteins of Eco-RNa-se ... protrusion. The RNA strand cannot fit in this groove, because a 2¢-OHgroup of RNA clashes with the indole ring of Trp221.Therefore, these two DNA binding sites probably con-tribute to the specificity for...
- 12
- 313
- 0
Tài liệu Báo cáo khoa học: Neuronal growth-inhibitory factor (metallothionein-3): reactivity and structure of metal–thiolate clusters* doc
... is a soft ligand and therefore shows a preference for soft metals. More-over the structures of metallothioneins are not rigid and hence there is little selectivity concerning the size of the ion. ... 2 forms could yield information on the structural and chemical features responsible for the biologicalpeculiarities of MT3, such as its growth-inhibitory activity. The fact that synthesis of ... dynamics of the protein structure and its implication for the biologicalfunction, see the minireview by Huang et al. in thisminireview series [29].What is the reason for the higher dynamics of the MT3...
- 10
- 569
- 0
Từ khóa: tài liệu báo cáo khoa học bản chất của khủng hoảng kinh tế thế giới pdflam the nao de tom tat bao cáo khoa hocbáo cáo khoa họcbáo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcNghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngchuyên đề điện xoay chiều theo dạngNghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANPhát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longNghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíChuong 2 nhận dạng rui roTổ chức và hoạt động của Phòng Tư pháp từ thực tiễn tỉnh Phú Thọ (Luận văn thạc sĩ)Tranh tụng tại phiên tòa hình sự sơ thẩm theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn xét xử của các Tòa án quân sự Quân khu (Luận văn thạc sĩ)chuong 1 tong quan quan tri rui roNguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtHIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀMTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ