Báo cáo khoa học: The multifarious short-term regulation of ammonium assimilation of Escherichia coli: dissection using an in silico replica pdf

... 1983 The multifarious short-term regulation of ammonium assimilation of Escherichia coli: dissection using an in silico replica Frank J. Bruggeman1, Fred C. Boogerd1and Hans V. Westerhoff1,2,31 ... the dynamics of the network in terms of a set of nonlinearordinary differential equations.Mass-ﬂow description of the UTase subnetworkand binding of a-KG to PIIAny of the three subunits of ... calculatedresults of PII removal indicated that ATase alone maybe insufﬁcient for regulating the level of ammonium assimilation upon an ammonium pulse.Analysis of regulation of the transient response of the...

Tài liệu Báo cáo khoa học: The cellulosomes from Clostridium cellulolyticum Identiﬁcation of new components and synergies between complexes ppt

... new proteins is based on the GH family of the catalytic module and the modular organization of the protein, or on the identity of the catalytic domain with another characterized protein (see text).Protein ... using the MALDI-TOF technique, except for the proteins 6, 7a and 7b, and 10 which were identiﬁed using the MS ⁄ MS technique. The modular structure of new proteins was determined by performing ... F5 was the most efﬁcient fraction,and the least active fractions, F1 and F2, showed lessthan half the activity of F5. All in all, these resultsindicate that the differences in the protein compositionof...

Báo cáo khoa học: The 2-oxoacid dehydrogenase multi-enzyme complex of the archaeon Thermoplasma acidophilum ) recombinant expression, assembly and characterization docx

... machinery is able to rec-ognize the lipoyl-domain of the Thermoplasma E2enzyme, the target lysine of which is flanked by D and Vresidues, as it is in the E2 protein of the E. coli OGDHCand of ... recombinant proteinsto assemble into an a2b2enzyme that catalyzes the decarboxylation of the branched-chain 2-oxoacids andpyruvate [14]. In the current paper, we report the clon-ing and ... [34] using the programSEDFIT, and the data were then directly fitted using the finite element solution of the Lamm equation in SEDFIT [18] to givevalues of the sedimentation coefficient in the...

Báo cáo khoa học: The retinoid-X receptor ortholog, ultraspiracle, binds with nanomolar afﬁnity to an endogenous morphogenetic ligand pptx

... 5 which forms one of the internal borders of the ligand-binding pocket. The side chain of W318 itself isdirected into the ligand-binding pocket. Thus, the trypto-phan is in a position to potentially ... farnesoate bindingis different to than that upon JH III binding. Wepreviously tagged the transcription-modulating AF2group of the ligand-binding domain using a pair of reporter tryptophan residues ... static quench-ing by the ligand; (b) change in its local environment bychange in position of amino acid side chains in the neigh-borhood of the indole group, where the change in localenvironment...

Báo cáo khoa học: The CRIPTO/FRL-1/CRYPTIC (CFC) domain of human Cripto Functional and structural insights through disulﬁde structure analysis docx

... 2A1), and thereforethat the CFC domain is sufficient for the interaction of Cripto with ALK4.To verify the role of the CFC domain in ALK4 binding,we analyzed the effects of point mutations in the ... maturehuman Cripto contains 12 cysteine residues, six in the EGFdomain and six in the CFC domain. To test whether the CFC domain could retain its function independent of the EGF domain, we generated ... was inactive in a rescue model of the oep phenotype in zebrafish [19]. These findings highlight the biologicalimportance of Cripto and underline the functional signi-ficance of the CFC domain. In...

Tài liệu Báo cáo khoa học: The central role of CDE/CHR promoter elements in the regulation of cell cycle-dependent gene transcription pdf

... proteinswith the promoter. E2F4 binding during the cell cyclecoincides with the binding of p107 or p130 [61,85].Another E2F site distal to the E2F ⁄ CDE acts as an activating element binding ... composed of p130, E2F4 ⁄ 5 and DP1 ⁄ 2 and a module containing the MuvB proteins Lin-37, Lin-52, Lin-54 and chromatin-associated Lin-9 and Lin-53 ⁄ RBBP4 ⁄ RbAp48.Furthermore, A-Myb and B-Myb ... In anotherreport, depletion of Lin-9, Lin-37, Lin-52 or Lin-54 byRNAi also led to a repression of genes, such as cyclinA and cyclin B1, in dividing cells [92]. Furthermore,depletion of Lin-9...

Tài liệu Báo cáo khoa học: The subtle side to hypoxia inducible factor (HIFa) regulation pdf

... MAPK.IntroductionSpurred-on by the discovery of their involvement in the pathophysiology of many disease states including cerebraland pulmonary ischemia, cancer tumourigenesis andmalignancy [1], the bHLH-PAS domain-containinghypoxia-inducible ... In contrast to these ﬁndings, the two cytokines were reportedto act in the same manner to increase transcription of HIF1a mRNA by twofold to threefold resulting in increasedprotein levels in synovial ... activity increases HIF transcriptionalactivation by increasing the pool of available HIFa proteinwithin the cell. The use of chemical inhibitors such aswortmannin and LY 294002 that block the phosphatidyl-inositol...

Báo cáo khoa học: The catalysis of the SARS 3C-like protease is under extensive regulation by its extra domain doc

... middle of the dimericenzyme. In fact in the core region, only two direct in- termonomer contacts exist, namely between Glu290 of one protomer and Arg4 of another. Indeed, the importance of the ... regulating the catalytic machin-ery. It would be of signiﬁcant interest in the future tointroduce the mutations into viral infectious clones totest whether these mutations will increase the viral ... Arg4–Glu290 interaction in dimeri-zation has been reported previously [22]. The role of the identiﬁed residues other than Arg4 and Glu290 in dimerization might be in maintaining the correct side-chain...

Báo cáo khoa học: The sigma factors of Mycobacterium tuberculosis: regulation of the regulators potx

... C-terminaldomain, possibly involved in sensing an in vivo stimulusfor the release of rKand an intracellular N-terminaldomain involved in interaction with rK. Certain muta-tions in M. ... factor- a-a, interleukin-1b, interleukin-6and interferon-c in the lungs of the infected animal[51]. The attenuation of the sigC mutant may resultfrom dysregulation of expression of several key ... result in major changes in the protein structure and folding [16], thereby making itnonfunctional. The stimulus and the mechanism thatlead to the release of rKfrom RskA remain unknown. The mode...

Báo cáo khoa học: Gonadotropin-releasing hormone: regulation of the GnRH gene pot

... have therefore variedaccording to the time at which the animals werekilled, the dose and duration of estrogen treatment,and the region of the brain analyzed [75]. During the estrous cycle, the ... Phosphorylation of Oct-1 inhibits the Oct-1 binding activity [125], thus the repression of GnRH-I gene expression [31].Retinoic acid (RA) The role of vitamin A and its derivative RA in the maintenance of ... regulation, including essen-tial cis-acting elements in the promoter, and also the interaction of transcription factors in achieving the basal expression levels. In addition, other components of the...

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