Báo cáo khoa học: Caspase-2 is resistant to inhibition by inhibitor of apoptosis proteins (IAPs) and can activate caspase-7 pot

... 2005 FEBS Caspase-2 is resistant to inhibition by inhibitor of apoptosis proteins (IAPs) and can activate caspase-7 Po-ki Ho1,2,3, Anissa M. Jabbour1,2,3, Paul G. Ekert1,4,5 and Christine J. ... (orempty vectors) were transformed into yeast and transformants spotted onto inducingmedium to visualize their impact on yeastgrowth. Caspase-2 can activate caspase-7 and is resistant to IAPs P ... mammalian inhibitor of apoptosis proteins (IAPs). Caspase-2 was able to acti-vate caspase-7, suggesting that caspase-2 can functionas an apical caspase.ResultsHigh level expression of pro-caspase-2...

Tài liệu Báo cáo khoa học: Bacitracin is not a speciﬁc inhibitor of protein disulﬁde isomerase pptx

... Bacitracin is not a specific inhibitor of protein disulfideisomeraseAnna-Riikka Karala and Lloyd W. RuddockBiocenter Oulu and Department of Biochemistry, University of Oulu, FinlandIntroductionProtein ... PDI inhibitors and specific antibodies haveoften been used to discover the function of PDI-like proteins, especially outside the ER. Bacitracin is acommonly used inhibitor in these studies, and ... and it is usu-ally considered to be a specific inhibitor of PDI activ-ity [6–8]. However, in vitro evidence for the action of bacitracin as an inhibitor of PDI is scarce, and evi-dence of its...

Báo cáo khoa học: MPP3 is recruited to the MPP5 protein scaffold at the retinal outer limiting membrane ppt

... similar to thecorresponding preimmune yolk and was not used forfurther immunohistochemical studies (data not shown).Immunohistochemistry and confocal laser scanningmicroscopy were used to determine ... endogenous MPP5 can be detected as 70 kDa band in cell lysates, but upon coimmunoprecipitation with MPP3 it is visible as double band of 70 and 80 kDa, due to enrichment of the 80 kDa band. Overexpressed ... existence of a complex between the two proteins. This hypothesis was tested by immuno-precipitation of Mpp4 from mouse retinal lysates usingAK4 antibody. Dlg1 was visualized as a double bandA...

Tài liệu Báo cáo khoa học: P25a ⁄ TPPP expression increases plasma membrane presentation of the dopamine transporter and enhances cellular sensitivity to dopamine toxicity pptx

... empty vector, DAT ⁄ P25a and DAT ⁄ a-syn to obtain equal concentrations of vector DNA wereincubated in the absence and presence of 0.5 mM DA for 24 h and subjected to analysis of viability by the ... analysis of DA and serotonin uptake, respectively.3[H]-DA was used to determine Vmaxcurves for DAT and NET, as3[H]-DA can also be used by NET, and 3[H]-serotonin was used for determination of ... hypothesized to play a major role in thisselective loss of dopaminergic neurons, and the cyto-solic fraction of DA is considered particularly toxic[3,7,26]. This highlights the importance of...

Tài liệu Báo cáo khoa học: "A Composite Kernel to Extract Relations between Entities with both Flat and Structured Features" ppt

... N1 and N2 are the sets of nodes in trees T1 and T2, respectively, and ()isubtreeIn is a function that is 1 iff the subtreei occurs with root at node n and zero otherwise, and ... 1()nc n is the child number of 1n , ch(n,j) is the jth child of node n and λ(0<λ<1) is the de-cay factor in order to make the kernel value less variable with respect to the subtree ... occurrence of each sub-tree without considering the layer and the ancestors of the root node of the sub-tree, our method is not limited by the constraints (identi-cal layer and ancestors for...

Báo cáo khoa học: "FROM N-GRAMS TO COLLOCATIONS AN EVALUATION OF XTRACT" pot

... lists are then sent off to the dictionary and go through several rounds of careful analysis before actually being submitted to the dictionary. The ratio of proposed can- didates to good candidates ... as the precision of Xtract is raised from 40% to 80% with a recall of 94%. By combining statistical techniques with a sophis- ticated robust parser we have been able to design and implement ... Evaluation of retrieval systems is usually done with the help of two parameters: precision and recall [Salton, 1989]. Precision of a retrieval system is defined as the ratio of retrieved...

Báo cáo khoa học: pyr RNA binding to the Bacillus caldolyticus PyrR attenuation protein – characterization and regulation by uridine and guanosine nucleotides potx

... RNA, as shown by thefailure of a control RNA (i.e. the antisense strand to BcBL1) to bind to any concentration of PyrR tested(Fig. 2A).Binding of PyrR to BcBL2 and BcBL3 in standardbinding ... modulators of PyrR binding to pyr attenua-tor region RNA, and hence the primary regulators of pyrimidine biosynthesis. Uridine nucleotide stimulation of RNA binding is easily understood in terms of ... Analysis of the binding of 32P-labeled BcBL2 to PyrR by the electrophoretic gel mobility shift method in the absence of effector (A) and in the presence of 500 lM GMP (B). The concentration of...

Báo cáo khoa học: Using directed evolution to improve the solubility of the C-terminal domain of Escherichia coli aminopeptidase P Implications for metal binding and protein stability pptx

... that of DHFR. Theresulting mutant fusion proteins can again be expressedin E. coli, and TMP resistance can be monitored. Thegenes of mutants that confer increased TMP resistanceare isolated and ... ligand to a residue that is unli-kely to bind metal. The G270V residue is located next to a metal-binding residue ) this mutation is likely to cause a conformational change that is likely to ... Schenk2 and David Ollis11 Research School of Chemistry, Australian National University, Canberra, Australia2 School of Molecular and Microbial Sciences, University of Queensland, Brisbane,...

Báo cáo khoa học: "A concurrent approach to the automatic extraction of subsegmental primes and phonological constituents from speech" docx

... to be recessive. This is handled by establishing in cue space a central location and within-cluster variance for each prime. The training sample needed for this consists of segments in which ... transforms (FFTs) of speech signals in terms of total amplitude or energy distribution ED across low, middle and high frequency parts of the vocal range and the angular frequencies to( F) and amplitudes ... h and ?. 1. Maalte¢ 2. Pbenttlelt Figure 1. Stagewise cue invocation strategy This launches the calculation of cues 4)5 (for the automatic segmentation process) and 4)6 (to distinguish...

Tài liệu Báo cáo khoa học: High affinity copper binding by stefin B (cystatin B) and its role in the inhibition of amyloid fibrillation docx

... related and have no rele-vance to in vivo function of the protein and evenless to amyloid fibrillation. As for the function[10,11,18], this is open to more research. The proteindecreases apoptosis ... myoclonusepilepsy of the Unverricht–Lundborg type. In studies of CSTB-deficient mice, lack of this inhibitor wasfound to be associated with signs of cerebellar granularcell apoptosis [20]. The mice ... to the contribution of tyrosines to this region of the spectrum [28].Figure 4B shows the near UV CD spectra of stefin A, stefin B, and the P79S mutant. It can be seenthat spectra of stefin B and...

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