... family member protein from a psychrotrophic bacteriumShewanellasp. SIB1 in cold-adaptationYutaka Suzuki, Mitsuru Haruki*, Kazufumi Takano, Masaaki Morikawa and Shigenori KanayaDepartment of ... theNdeI–BamHI sites of pET-2 8a. This DNA fragment wasamplified by PCR. The sequences of the PCR primers were5¢-AGAGAGAATTCATATGTCAGATTTGTTCAG-3¢for the 5¢-primer and 5¢-GGCCACTGGATCCAACTACAGCAATTCTCA-3¢ ... composed of a N-terminal domain, that is involved in dimerization of the protein, and a C-terminal catalyticdomain [46]. Three helices (a1 –3) comprise the N-terminaldomain, and six b-strands (b1–6)...