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Báo cáo khoa học: Solution structure of long neurotoxin NTX-1 from the venom of Naja naja oxiana by 2D-NMR spectroscopy pot

Báo cáo khoa học: Novel c-carboxyglutamic acid-containing peptides from the venom of Conus textile docx

Báo cáo khoa học: Novel c-carboxyglutamic acid-containing peptides from the venom of Conus textile docx

... ionmass spectrum of the doubly charged ion at m ⁄ z ¼660.18. The isotopic distribution of the b2ion (inset)indicates the presence of bromine. The MS ⁄ MSspectrum allows assignment of the sequenceSW*NCYNGHCTG, ... methyl-esterification of the fragment (Fig. S3). The presence of six cysteinyl resi-dues was confirmed by observation of an average massincrement of 640.5 Da after pyridylethylation of the reduced peptides ... Conus c-carboxylase and synthetic peptide sub-strates localized the c-CRS of Gla(1)–TxVI to the region )14 to )1 of the polypeptide precursor: the Kmwas reduced from 1.8 mm for Gla (1)–TxVIlacking...
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Tài liệu Báo cáo khoa học: Erythrochelin – a hydroxamate-type siderophore predicted from the genome of Saccharopolyspora erythraea docx

Tài liệu Báo cáo khoa học: Erythrochelin – a hydroxamate-type siderophore predicted from the genome of Saccharopolyspora erythraea docx

... characterization of these compounds isgrowing and may result in the synthesis of specificinhibitors based on the structure of the pathogensiderophore [16].A promising approach for the isolation of secondarymetabolites, ... composed of a hOrn and a haOrn moiety.Furthermore, a long- range1H–13C correlation wasdetected between the carbonyl carbon of the serine and the d-CH2 of the hOrn, which constitutes the DKP.Therefore, ... S18). The domain organization and the predicted substrate specificities of the A-domains donot reflect in the structure of erythrochelin andexclude its biosynthesis by Nrps3. The extraction of culture...
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Báo cáo khoa học: A Kazal prolyl endopeptidase inhibitor isolated from the skin of Phyllomedusa sauvagii pdf

Báo cáo khoa học: A Kazal prolyl endopeptidase inhibitor isolated from the skin of Phyllomedusa sauvagii pdf

... for each concentration of the inhibitor by nonlinear fit of the data from the abovenegative controls; [I] is the concentration of the inhibitor,IC50is the concentration of inhibitor that causes ... 7.4. Then, proteolysis was started by the addition of 50 lLof10mgÆmL)1a-casein. At different time-points,60 lL aliquots were withdrawn and the reaction wasstopped by the addition of 60 lLof1.8Mtrichloroaceticacid. ... þS½EC50bð4Þwhere [S] is the concentration of the sample, EC50is the effective concentration that causes 50% of the effect, andb represents the cooperativity of the effect.HemagglutinationHamster,...
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Báo cáo khoa học: Solution structure of long neurotoxin NTX-1 from the venom of Naja naja oxiana by 2D-NMR spectroscopy pot

Báo cáo khoa học: Solution structure of long neurotoxin NTX-1 from the venom of Naja naja oxiana by 2D-NMR spectroscopy pot

... Solution structure of long neurotoxin NTX-1 from the venom of Naja naja oxiana by 2D-NMR spectroscopy Mehdi Talebzadeh-Farooji1, Mehriar Amininasab1, ... revealed that the solution structure is more flexible in the tip of loop II [14].Here we report the elucidation of solution structure of neurotoxin- 1 (NTX-1; P01382, from Naja naja oxiana) viatwo-dimensional1H-NMR ... although the length of loop I is longer in short neurotoxins.TheNMRstructureofNTX-1isclosesttothatofthea-cobratoxin crystal. The superposition of these twostructures shows an RMSD value of 3.00...
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Tài liệu Báo cáo khoa học: Solution structure of hirsutellin A – new insights into the active site and interacting interfaces of ribotoxins docx

Tài liệu Báo cáo khoa học: Solution structure of hirsutellin A – new insights into the active site and interacting interfaces of ribotoxins docx

... mecha-nism of transphosphorylation, which implies the ABFig. 3. Stereo diagram of the active center of HtA. (A) Superposition of the active-siteresidues of the 20 conformers of the solu-tion structure ... electrostatically with the othercharged groups. In the NMR structures, this sidechain of D40 is a short distance (£ 3A˚) from the sidechains of H42 and R95. Finally, the aromatic ring of F126, placed ... second-ary structure elements. As in other ribotoxins, theseABCFig. 2. Representation of the 3D structure of HtA in solution. (A) Superposition of the 20 best structures obtained in this work(PDB...
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Tài liệu Báo cáo khoa học: Solution structure of crotamine, a Na+ channel affecting toxin from Crotalus durissus terrificus venom docx

Tài liệu Báo cáo khoa học: Solution structure of crotamine, a Na+ channel affecting toxin from Crotalus durissus terrificus venom docx

... fact, in the structures of Crt, the side-chain of Phe 25 is close enough to the amide proton of Lys38 to induce a ring current effect.Description of the toxin structure and potential surface The ... doi:10.1046/j.1432-1033.2003.03563.xTo the best of our knowledge, this is the first report on the solution structure of a toxin from snake venom active onNa+channel.Materials and methodsIsolation of crotamineNative ... reported in Fig. 3A,and the analysis of the / and w angle distribution allowed the identification of the type and boundaries of the secondary structure elements. Crt consists of three antiparal-lel...
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Tài liệu Báo cáo khoa học: Solution structure of the matrix attachment region-binding domain of chicken MeCP2 ppt

Tài liệu Báo cáo khoa học: Solution structure of the matrix attachment region-binding domain of chicken MeCP2 ppt

... [15]. The solution structure of the MBD of rat (r)MeCP2 hasrecently been determined [17]. The MBD adopts a wedge-shaped structure, composed of an antiparallel b-sheet onone face of the wedge ... one-turn helix on the other face. It is thoughtthat the two inner strands of the b-sheet lie within the majorgroove of the DNA and that a hydrophobic pocket formed by the side chains of Y123 and ... [18]. The structure of the helicalcoil a2/a3allows us to interpret the consequences of the six mutations. As P153(152) and G162(161) are buried in the protein core, replacement of each of these...
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Báo cáo khoa học: Solution structure of the catalytic domain of RICH protein from goldfish pot

Báo cáo khoa học: Solution structure of the catalytic domain of RICH protein from goldfish pot

... similarity of the structures. The lowest-energy structure from the RICH NMR ensemble is used for the overlay. (D) The surface of the RICH catalytic domain shows several negatively charged patches of ... occupied by the longesthelices a1 and a9. A number of short helical fragmentsgroup together in the vicinity of the N-terminus. Thishelical patch is the most basic part of the moleculeand a potential ... regrowthupon the optic nerve crush, and also expressed in the germinal neuroepithelium of retina, which generatesnew neurons throughout the lifespan of the fish [11]. The cloning of the RICH proteins from...
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Báo cáo khoa học: Solution structure of the active-centre mutant I14A of the histidinecontaining phosphocarrier protein from Staphylococcus carnosus ppt

Báo cáo khoa học: Solution structure of the active-centre mutant I14A of the histidinecontaining phosphocarrier protein from Staphylococcus carnosus ppt

... towards the interior of the protein. The space that in the wild-typemolecule is occupied by the large hydrophobic side chain of Fig. 3. Structure ensemble of HPr(I14A). The average structure of the 10 ... wascomputed as the ratio of the s tandard deviations of the chemical shifts of the amide nitrogen and p roton nuclei.ResultsDetermination of the three-dimensional structure of HPr(I14A)To allow the comparison ... marked by black dots and indicate residues for which the absolute value of the difference in dihedral angles exceeds the sum of the errors.Ó FEBS 2004 Solution structure of the I14A mutant of HPr...
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Báo cáo khoa học: Solution structure and backbone dynamics of the XPC-binding domain of the human DNA repair protein hHR23B docx

Báo cáo khoa học: Solution structure and backbone dynamics of the XPC-binding domain of the human DNA repair protein hHR23B docx

... vectorsalong the peptide chain. We analyzed the diversehydrophobic surfaces of XPCB–hHR23B, which result from the flexible N-terminal region, and attempt todetermine the structure of the XPC-binding ... or kinks (Figs 1and 2A). The N-terminal part of XPCB–hHR23BABCFig. 2. NMR structure of the XPCB domain of hHR23B. (A) Stereoview of the 12 superimposed structures of XPCB–hHR23B. All Pro resi-dues ... XPCB–hHR23A in the Kamionka and Feigon study [20].Moreover, the HT1 region was not observed in an ear-lier structure of XPCB–hHR23A reported by Waterset al. [13]. The effect of the motion of the N-terminalregion...
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